GABA transporter 1 transcriptional starting site exhibiting tissue specific difference

INTRODUCTIONIn the vertebrate central nervous system (CNS),GABA transporters (GAT) are believed to play animportant role in termination of GABAergiC transInission. GATI was the first cloned member of neurotransmitter transporters superfanilly[1], and soon,other three subtypes (GAT2-4) were subsequentlycloned. Since GABA is the predominant inhibitoryneurotranslliltter in CNS, abnormallty of GATs hasa direct relationship with certain kinds of nervousdisorders, such as epilepsy a…     

白音华矿区土壤重金属含量的空间异质性

为探讨开矿对白音华矿区土壤重金属空间分布的影响,本研究以内蒙古西乌珠穆沁旗白音华煤矿区周边土壤为对象,分析了距离矿区8 km内的重金属Cu、Cr、Pb和Mn含量的空间异质性.结果表明:土壤重金属Cu、Cr、Pb和Mn的平均含量分别为12.7、32.6、29.9和201.3 mg ? kg-1,其变异系数分别为26.8％...     

不同密度樟子松人工林土壤碳氮磷化学计量特征

以科尔沁沙地不同密度(490、750、1550、1930、2560株·hm-2)樟子松人工林(栽植于1980年)为研究对象,分析林分密度对土壤碳、氮、磷浓度及其计量比的影响,研究林分密度与土壤养分状况的关系。结果表明:随着樟子松林密度增加,各土层(0～10、10～20和20～40 cm)土壤有机碳、全氮、全磷浓度和C∶N呈先增加后降低趋势,而土壤有效磷浓度呈先降低后增加趋势。土壤有机碳浓度在490株·hm-2密度小于其他密度,而有效磷浓度大于其他密度;土壤C∶P和N∶P在2560株·hm-2密度显著大于其他密度。各密度樟子松林土壤有机碳、全氮、全磷和有效磷浓度在0～10 cm土层显著大于10～20和20～40cm土层,樟子松人工林土壤养分具有表聚性。通过典范对应分析发现,密度对樟子松林土壤养分影响的主要因子是土壤有机碳、全氮和全磷,且密度为1550株·hm-2时土壤有机碳、全氮、全磷和碱解氮浓度较高,而C∶P和N∶P较低。因此,当樟子松人工林密度为1550株·hm-2时,土壤养分浓度较高,林木生长较好,为最佳经营密度。     

大鼠子宫内膜异位模型的建立与组织学观察

目的为开发诊治子宫内膜异位症(endometriosis,EMT)的新药研究提供理想的动物模型。方法取雌性未交配性成熟大鼠,术前雌激素诱导,麻醉开腹取部份右侧子宫,将内膜种植于左腹壁内,术后16周取出包块,进行组织形态学、组织化学观察。结果异位内膜在腹壁内生长,呈隆起囊状小包块,内有黏液,具有正常子宫内膜基本组织结构,囊腔较大。异位内膜中有糖原、RNA的存在。结论该手术方法建立的子宫异位内膜生长良好,术后一周就可摸及包块大小,为开发研究子宫内膜异位症的新药提供了方便。     

垂体瘤转化基因1(PTTG1)的表达变化对人前列腺癌细胞LNCaP生长增殖的影响

垂体肿瘤转化基因1(PTTG1)具有促进肿瘤生长和转移的作用.通过上调或下调基因表达的策略,观察PTTG1基因对人前列腺癌细胞株LNCaP细胞生长增殖的影响.利用PCR技术分离出PTTG1全长cDNA,分别正向和反向插入真核表达载体pIRES2-EGFP,重组载体分别命名为正义PTTG1-S/pIRES2-EGFP(即pI-P-S)和反义PTTG1-AS/pIRES2-EGFP(即pI-P-AS),将这两种重组载体稳定转染LNCaP细胞,通过流式细胞仪和MTT法分别检测了细胞周期和细胞增殖的情况.转染正义PTTG1后处于S期和G2期的细胞明显增加,细胞生长增殖能力增强;相反,转染反义PTTG1后处于S期和G2期细胞明显减少,细胞生长增殖能力减弱(P<0.05).结果表明,PTTG1能明显改变人前列腺癌细胞株LNCaP的细胞周期和细胞生长增殖能力,它的异常表达可能参与前列腺癌细胞生长增殖过程.     

Gene and domain duplication in the chordate Otx gene family: insights from amphioxus Otx

We report the genomic organization and deduced protein sequence of a cephalochordate member of the Otx homeobox gene family (AmphiOtx) and show its probable single-copy state in the genome. We also present molecular phylogenetic analysis indicating that there was single ancestral Otx gene in the first chordates which was duplicated in the vertebrate lineage after it had split from the lineage leading to the cephalochordates. Duplication of a C-terminal protein domain has occurred specifically in the vertebrate lineage, strengthening the case for a single Otx gene in an ancestral chordate whose gene structure has been retained in an extant cephalochordate. Comparative analysis of protein sequences and published gene expression patterns suggest that the ancestral chordate Otx gene had roles in patterning the anterior mesendoderm and central nervous system. These roles were elaborated following Otx gene duplication in vertebrates, accompanied by regulatory and structural divergence, particularly of Otx1 descendant genes.      

圆瓣姜花根茎挥发油的化学成分

利用水蒸汽蒸馏法提取圆瓣姜花根茎挥发油,运用毛细管气相色谱-质谱联用法对挥发油进行了分析,分离出60个峰,鉴定了其中的51种成分,所鉴定成分占挥发油总量的97.32%,其主要化学成分为单萜及倍半萜类化合物。     

复方环丙酮胺治疗浅部真菌病的疗效观察

观察复方环丙酮胺喷剂治疗浅部真菌病的疗效。第Ⅰ批给予复方环丙酮胺喷剂,1次/d喷于患处,用药4周后观察疗效;第Ⅱ批随机入A、B组,A组给予复方环丙酮胺喷剂+曲安奈德喷剂,B组给予复方环丙酮胺喷剂,均1次/d喷于患处,用药2周、4周后观察疗效。第Ⅰ批治疗4周总有效率为80%;第Ⅱ批A、B组治疗2周总有效率分别为75%及82.35%,治疗4周总有效率分别为94.74%及100%。复方环丙酮胺喷剂和复方环丙酮胺喷剂+曲安奈德喷剂治疗浅部真菌病的疗效相似(P>0.05),且疗效显著、局部刺激小、瘙痒缓解明显、安全性较高。     

Identification of over-expressed genes in human renal cell carcinoma by combining suppression subtractive hybridization and cDNA library array

Renal cell carcinoma (RCC) is a common uro- genital malignancy and often shows odd biological features. RCC accounts for approximately 2% of ma- lignancies worldwide. The incidence of and mortality from RCC have continuously increased during the last 50 years. One third of the patients already have me- tastases when first consulting the doctors. Another 30%—40% of patients develop metastasis after surgi- Identification of over-expressed genes in human RCC 149 cal excision of the pri…     

Clinical Burkholderia pseudomallei isolates from north Queensland carry diverse bimABm genes that are associated with central nervous system disease and are phylogenomically distinct from other Australian strains

BackgroundBurkholderia pseudomallei is an environmental gram-negative bacterium that causes the disease melioidosis and is endemic in many countries of the Asia-Pacific region. In Australia, the mortality rate remains high at approximately 10%, despite curative antibiotic treatment being available. The bacterium is almost exclusively found in the endemic region, which spans the tropical Northern Territory and North Queensland, with clusters occasionally present in more temperate climates. Despite being endemic to North Queensland, these infections remain understudied compared to those of the Northern Territory.Methodology/Principal findingsThis study aimed to assess the prevalence of central nervous system (CNS) disease associated variant bimA_Bm, identify circulating antimicrobial resistance mutations and genetically distinct strains from Queensland, via comparative genomics. From 76 clinical isolates, we identified the bimA_Bm variant in 20 (26.3%) isolates and in 9 (45%) of the isolates with documented CNS infection (n = 18). Explorative analysis suggests a significant association between isolates carrying the bimA_Bm variant and CNS disease (OR 2.8, 95% CI 1.3–6.0, P = 0.009) compared with isolates carrying the wildtype bimA_Bp. Furthermore, 50% of isolates were identified as novel multi-locus sequence types, while the bimA_Bm variant was more commonly identified in isolates with novel sequence types, compared to those with previously described. Additionally, mutations associated with acquired antimicrobial resistance were only identified in 14.5% of all genomes.Conclusions/SignificanceThe findings of this research have provided clinically relevant genomic data of B. pseudomallei in Queensland and suggest that the bimA_Bm variant may enable risk stratification for the development CNS complications and be a potential therapeutic target.