Phytoplankton and zooplankton of the Westport River Estuary,Massachusetts (USA)

Zooplankton and phytoplankton samples were simultaneously collected at approximately biweekly intervals over most of an annual cycle in the Westport River Estuary, Massachusetts. Phytoplankton numbers were overwhelmingly dominated throughout the study by athecate nanoplankton <5 µm in diameter. The zooplankton was primarily composed of copepod nauplii. Periods of occurrence of other zooplankters such as adult copepods, marine cladocerans, meroplankters and ctenophores were similar to those recorded for adjacent estuaries. Our results emphasize the abundance of smaller plankters that have been historically undersampled.     

On Wolff's law of trabecular architecture.

Several studies suggest that the yield strain in cancellous bone may be uniformly distributed and isotropic. Yield strain was reported to be independent of textural anisotropy in bovine cancellous bone [Turner, J. biomech. Engng 111, 1-5 (1989)] and it is plausible that yield strain is isotropic in human cancellous bone as well. In this paper, it is hypothesized that uniform, isotropic strain represents a goal of cancellous bone adaptation, i.e. cancellous bone alters its structure to maintain uniform, isotropic peak strains. Therefore, textural anisotropy must exactly cancel the anisotropy of the peak principal stresses imposed upon cancellous bone. When evaluating the relationships between mechanical properties of cancellous bone and trabecular architecture, it was found that over 90% of the variance of yield strength can be explained by one term--rho 2H3 (where rho is apparent density and H is the normalized anisotropy (fabric) constant). Furthermore, this single term explains 70-78% of the variance in Young's modulus of cancellous bone. Based upon these findings, it was postulated that fabric adaptation goes as Hi/Hj = [ sigma i/sigma j[, where Hi and Hj are fabric eigenvalues in the i- and the j-direction and sigma i and sigma j are peak principal stresses.     

In vivo human somatic mutation: frequency and spectrum with age.

The number and molecular nature of in vivo mutations in relation to age was studied at the autosomal HLA-A locus in human lymphocytes. Mutant lymphocytes were isolated by immunoselection, cloned at limiting dilution and enumerated, and the HLA-A gene and other polymorphic gene loci on chromosome 6 were studied by Southern blotting to determine gene dosage and loss of heterozygosity. Results of 167 assays in 73 individuals showed that the total number of mutant lymphocytes increased significantly with age from a geometric mean frequency of 0.71 x 10(-5) in neonates to 6.53 x 10(-5) in elderly individuals. Analysis of rearrangement of T lymphocyte receptor beta or gamma chain genes gave a best estimate of 3.3% for the proportion of mutant lymphocytes detected which are clonally related. Molecular study of 434 mutants from 31 individuals showed no change on Southern blotting in 64.7%, gene deletion in 2.8% and mitotic recombination in 32.5%. Two mutants due to gene conversion but no mutants due to non-disjunction were detected. The number of 'no change' and recombination mutants increased significantly with age. There was a significant difference between individuals in the proportion of mutants which resulted from mitotic recombination and the data suggested that the proportion was bimodally distributed. The point of crossing-over in recombination mutants was predominantly randomly distributed between the HLA-A locus and the centromere.     

Dark inhibition of ribulose-1,5-bisphosphate carboxylase/oxygenase in legumes: A biosystematic study

2-carboxyarabinitol 1-phosphate (CA 1-P) is a naturally occurring inhibitor of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). Members of the Fabaceae exhibit a particularly wide range in the extent of CA 1-P accumulation during darkness and include Phaseolus vulgaris, whose dark/light regulation of Rubisco activity is principally achieved by synthesis/degradation of CA 1-P. An extensive survey of the degree of dark inhibition of Rubisco was undertaken for the subfamily Papilionoideae to elucidate evolutionary patterns in the occurrence of this regulatory mechanism. Seventy-five species from 21 tribes were examined. Dark inhibition of Rubisco was found in ancestral tribes such as the Sophoreae, but was substantially reduced or absent in representative species of three more recently evolved tribes, Cicereae, Hedysareae and Vicieae. We conclude that regulation of Rubisco by CA 1-P is neither of recent origin nor of restricted distribution among the Papilionoideae. On the contrary, it becomes lost or less pronounced only in a minority of the more evolutionarily advanced species in this important subfamily.     

AN OVERVIEW OF THE GENUS PYRRHOPAPPUS (ASTERACEAE: LACTUCEAE) WITH EMPHASIS ON CHLOROPLAST DNA RESTRICTION SITE DATA

The genus Pyrrhopappus in recent systematic treatments has comprised five taxa (four species, one with two varieties), which have now been studied anew using morphogeographical and chloroplast DNA restriction site data. Eight populations, representing all of the recognized taxa of Pyrrhopappus, were digested with 17 restriction enzymes. Only three restriction site differences were found from among 750 restriction sites and no length variations were observed. This contrasts with similar studies, using these same enzymes, on the closely related genus Krigia in which 173 mutation sites and 20 length variations were found among the seven species concerned. Nucleotide sequence divergence values among the species of Pyrrhopappus were extremely low (0.0012) compared to much higher values found in the closely related genus Krigia (0.1270). Three species of Pyrrhopappus are herein recognized: two diploids with 2n = 12 chromosomes, P. carolinianus and P. pauciflorus (including P. multicaulis, P. geiseri and P. rothrockii), and a tetraploid (2n = 24), P. grandiflorus. The tetraploid is partially sympatric with both diploids but is readily recognized by its perennial roots, which bear tuber-like enlargements. These three species presumably arose relatively recently, and the DNA data suggest that neither P. pauciflorus nor P. carolinianus gave rise to the tetraploid P. grandiflorus.     

Taxonomy ofNeurolaena (Asteraceae-Heliantheae)

A taxonomic treatment of the tropical genusNeurolaena (tribeHeliantheae, subtribeGalinsoginae) is rendered. Ten species are recognized: the widespread, annual, weedy,N. lobata and nine relatively rare, relictual, shrubby or small tree species, mostly restricted to southern Mexico and Central America. Distributional maps, key to species, and complete synonymy are presented. A chromosomal and chemical review is also provided.     

Interaction of phlorizin and sodium with the renal brush-border membraned-glucose transporter: Stoichiometry and order of binding

Summary The order and stoichiometry of the binding of phlorizin and sodium to the renal brush-border membraned-glucose transporter are studied. The experimental results are consistent with a random-binding sites is one-to-one. When the kinetics of phlorizin binding are measured as a function of increasing sodium concentration no significant variation is found in the apparent number of binding sites; however, the apparent binding constant for phlorizin decreases rapidly from approximately 16 m at [Na]=0 to 0.1 m at [Na]=100mm and approaches 0.05 m as [Na]. The experimental data are fit to a random carrier-type model of the coupled transport of sodium andd-glucose. A complete parameterization of the phlorizin binding properties of this model under sodium equilibrium conditions is given.     

The use of a fabric softener in the reconstitution of mummified tissue prior to paraffin wax sectioning for light microscopical examination

A method of reconstituting mummified tissues using the fabric softener "Comfort" is described. Routine histological paraffin wax sections were prepared following overnight treatment at room temperature with a 0.2% solution of this substance in isotonic saline. A comparison of results obtained by this method is made with those obtained using Ruffer's and Sandison's solutions. Nuclear stains gave poor results whereas connective tissue stains demonstrated their respective fibers by all rehydration methods.