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31.
Samuel Gmez-Arcas Francisco Jos Muoz Adriana Ricarte-Bermejo ngela María Snchez-Lpez Marouane Baslam Edurne Baroja-Fernndez Abdellatif Bahaji Goizeder Almagro Nuria De Diego Karel Doleal Ondej Novk Jesús Leal-Lpez Rafael Jorge Len Morcillo Araceli G Castillo Javier Pozueta-Romero 《Plant physiology》2022,190(4):2137
32.
Eudald Carbonell Isabel Sauceda Ángel Carbajo Isabel Díaz Francisco J. García Marcos García Juan Gil Luz M. León Eva Mancha Rosa M. Merino Abel Morcillo Antonio Rodríguez Santiago Giralt 《L'Anthropologie》2005,109(2):267
The excavations carried out in the cave of Santa Ana (Cáceres, Spain), cave of the karstic network of the “Calerizo” of Cáceres, enabled us to know the existence, in stratigraphy, of the three lito-techniques modes which characterize the industrial development of lower and middle Pleistocene in the Iberian Peninsula. On standby of new research, the results obtained until now permit us two work out a diachronic assumption of technical evolution. In the Iberian Peninsula, there are only two karstic systems where we can fallow this technological development; one is the “Sierra de Atapuerca” (Burgos) and the other is the “Calerizo de Cáceres” (Cáceres). 相似文献
33.
Gemita Pizarro Beatriz Paz Sonsoles Gonzlez-Gil Jos M. Franco Beatriz Reguera 《Harmful algae》2009,8(6):926-937
This work describes and compares the seasonal variability of toxin profiles and content, estimated by LC–MS analyses, in picked cell of Dinophysis acuta Ehrenberg, in plankton concentrates rich in this species, and in extracellular lipophilic toxins collected by adsorbent resins during weekly sampling in a Galician ría (Western Iberia) from October 2005 to January 2006. Picked cells of D. acuta—which exhibited a fairly stable OA:DTX2 ratio, close to 3:2, but a variable okadaates:PTX2 ratio—showed a 9-fold variation in cell toxin quota, which was partly related to cellular volume, with maximum values (19 pg cell−1) observed during the exponential decline of the population. Large differences in toxin profiles and content were observed between picked cells and plankton concentrates (up to 73 pg cell−1 in the latter), that were most conspicuous after the bloom decline. The toxin profile of picked cells was more similar to that observed in the adsorbent resins than to the profiles of plankton concentrates. Their continued detection several weeks after the disappearance of Dinophysis spp. indicates that these toxins may take a long time to be degraded. It is concluded that analyses of picked-cells are essential to determine the contribution of each species of Dinophysis to a toxic outbreak. Estimates of cellular toxin content from plankton concentrates can lead to considerable overestimates after Dinophysis blooms decay due to extracellular toxins that persist in the water column, possibly bound to organic aggregates and detritus, and are retained (>0.22 μm) in the filters. 相似文献
34.
Helga Fernández Nicolás Prandoni Mercedes Fernández-Pascual Susana Fajardo César Morcillo Eduardo Díaz Manuel Carmona 《PloS one》2014,9(10)
Background
Endophytic bacteria that have plant growth promoting traits are of great interest in green biotechnology. The previous thought that the Azoarcus genus comprises bacteria that fit into one of two major eco-physiological groups, either free-living anaerobic biodegraders of aromatic compounds or obligate endophytes unable to degrade aromatics under anaerobic conditions, is revisited here.Methodology/Principal Findings
Light, confocal and electron microscopy reveal that Azoarcus sp. CIB, a facultative anaerobe β-proteobacterium able to degrade aromatic hydrocarbons under anoxic conditions, is also able to colonize the intercellular spaces of the rice roots. In addition, the strain CIB displays plant growth promoting traits such nitrogen fixation, uptake of insoluble phosphorus and production of indoleacetic acid. Therefore, this work demonstrates by the first time that a free-living bacterium able to degrade aromatic compounds under aerobic and anoxic conditions can share also an endophytic lifestyle. The phylogenetic analyses based on the 16S rDNA and nifH genes confirmed that obligate endophytes of the Azoarcus genus and facultative endophytes, such as Azoarcus sp. CIB, locate into different evolutionary branches.Conclusions/Significance
This is the first report of a bacterium, Azoarcus sp. CIB, able to degrade anaerobically a significant number of aromatic compounds, some of them of great environmental concern, and to colonize the rice as a facultative endophyte. Thus, Azoarcus sp. CIB becomes a suitable candidate for a more sustainable agricultural practice and phytoremediation technology. 相似文献35.
María Luisa Soto-Montenegro Santiago Pe?a-Zalbidea Jose María Mateos-Pérez Marta Oteo Eduardo Romero Miguel ángel Morcillo Manuel Desco 《PloS one》2014,9(11)
Purpose
The goal of this study was to compare the tumor uptake kinetics and diagnostic value of three 68Ga-DOTA-labeled somatostatin analogues (68Ga-DOTATOC, 68Ga-DOTANOC, and 68Ga-DOTATATE) using PET/CT in a murine model with subcutaneous meningioma xenografts.Methods
The experiment was performed with 16 male NUDE NU/NU mice bearing xenografts of a human meningioma cell line (CH-157MN). 68Ga-DOTATOC, 68Ga-DOTANOC, and 68Ga-DOTATATE were produced in a FASTLab automated platform. Imaging was performed on an Argus small-animal PET/CT scanner. The SUVmax of the liver and muscle, and the tumor-to-liver (T/L) and tumor-to-muscle (T/M) SUV ratios were computed. Kinetic analysis was performed using Logan graphical analysis for a two-tissue reversible compartmental model, and the volume of distribution (Vt) was determined.Results
Hepatic SUVmax and Vt were significantly higher with 68Ga-DOTANOC than with 68Ga-DOTATOC and 68Ga-DOTATATE. No significant differences between tracers were found for SUVmax in tumor or muscle. No differences were found in the T/L SUV ratio between 68Ga-DOTATATE and 68Ga-DOTATOC, both of which had a higher fraction than 68Ga-DOTANOC. The T/M SUV ratio was significantly higher with 68Ga-DOTATATE than with 68Ga-DOTATOC and 68Ga-DOTANOC. The Vt for tumor was higher with 68Ga-DOTATATE than with 68Ga-DOTANOC and relatively similar to that of 68Ga-DOTATOC.Conclusions
This study demonstrates, for the first time, the ability of the three radiolabeled somatostatin analogues tested to image a human meningioma cell line. Although Vt was relatively similar with 68Ga-DOTATATE and 68Ga-DOTATOC, uptake was higher with 68Ga-DOTATATE in the tumor than with 68Ga-DOTANOC and 68Ga-DOTATOC, suggesting a higher diagnostic value of 68Ga-DOTATATE for detecting meningiomas. 相似文献36.
M A Morcillo M I Rucandio J Santamaría 《Cellular and molecular biology, including cyto-enzymology》2000,46(2):435-444
Several studies have recently shown that metallothionein (MT), a protein characterized by a high thiol content and that binds Zn2+ and Cu+, might be involved in the protection against oxidative stress and can act as a free radical scavenger. Oxidative stresses, such as irradiation, increase lipid peroxidation (LP) and subsequent tissue damage through free radical production. The induction of hepatic MT synthesis by gamma-irradiation (20 Gy) at 8, 24, 30 and 48 hrs. post-irradiation in two different age groups of Sprague-Dawley rats (39-40 and 48-49 days old) was studied. LP measured by the thiobarbituric acid reactive substances (TBARS) assay and Cu and Zn levels in liver have also been determined. In the younger group, the gamma-irradiation induced hepatic MT synthesis and increased LP that peaked 24 hrs. after irradiation. During the first 30 hrs. post-irradiation, a positive and statistically significant correlation between hepatic MT content and LP level in liver was found. In the older group, liver MT synthesis was only increased 1.7-fold and LP levels were not altered at 24 hrs. post-irradiation compared with sham-irradiated rats.Therefore it appears that LP is not necessary for induction of MT synthesis by gamma-irradiation. 相似文献
37.
38.
Heidi Jonckheere Jean-Marc Taymans Jan Balzarini Sonsoles Veláazquez Maria Jose Camarasa Jan Desmyter 《Nucleosides, nucleotides & nucleic acids》2013,32(3-5):599-602
Abstract By introducing the TSAO-resistance mutation 138Glu→Lys in only the p51 subunit of HIV-1 RT, we obtained compelling evidence for a structural and functional role of the p51 subunit in the sensitivity and/or resistance of the enzyme to the TSAO-derivatives. 相似文献
39.
40.
Jouannic S Argout X Lechauve F Fizames C Borgel A Morcillo F Aberlenc-Bertossi F Duval Y Tregear J 《FEBS letters》2005,579(12):2709-2714
This is the first report of a systematic study of genes expressed by means of expressed sequence tag (EST) analysis in oil palm, a species of the Arecales order, a phylogenetically key clade of monocotyledons that is not widely represented in the sequence databases. Five different cDNA libraries were generated from male and female inflorescences, shoot apices and zygotic embryos and unidirectional systematic sequencing was performed. A total of 2411 valid EST sequences were thus obtained. Cluster analysis enabled the identification of 209 groups of related sequences and 1874 singletons. Putative functions were assigned to 1252 of the set of 2083 non-redundant ESTs obtained. The EST database described here is a first step towards gene discovery and cDNA array-based expression analysis in oil palm. 相似文献