Parasites of fishes in the recently inundated ephemeral Lake Liambezi,Namibia

Lake Liambezi forms the periodic connection between the upper Zambezi, Kwando and Okavango rivers. A full parasitological assessment was conducted on 86 fish, representing 14 species in six families sampled in August 2011. Parasite diversity was low and dominated by species with complex life cycles involving intermediate hosts. Most prevalent were larval nematodes (Contracaecum sp.) infecting 12 and Trypanasoma sp. infecting nine of the 14 host species. The intra-erythrocytic parasite Babesiosoma mariae was found in the blood of Coptodon rendalli and Oreochromis andersonii with prevalence of 50% and 60%, respectively. The host-specific monogenean Annulotrema hepseti was recorded only from H. cuvieri with a prevalence of 100%. Notable absences were the copepod and branchiuran parasites that have direct lifecycles and usually occur in high prevalence and abundance in the region. Because parasites with direct life cycles can only be transported into the lake on the host fish, their absence suggests limited immigration of infected fishes into the lake. This suggests that internal recruitment dominates over immigration in the fish population dynamics in Lake Liambezi.     

Critical review of guidelines against a systematic framework with regard to consistency on allocation procedures for recycling in LCA

Purpose

Multifunctionality in LCA can be solved by several allocation procedures. Various official guidelines give divergent recommendations in which allocation procedure to apply, and up to now, no consensus has been reached. We aim to identify the obstacles to a consistent allocation approach that can be applied to all product categories and is supported by a broad range of stakeholders.

Methods

Based on a systematic framework for consistent allocation, developed by Schrijvers et al. (Int J Life Cycle Assess, 2016), we identify five review criteria that indicate the degree of consistency in the proposed allocation procedure of official guidelines. Several relevant guidelines, i.e. ISO 14044, ISO/TR 14049, ISO/TS 14067, the ILCD Handbook, BP X30-323-0, PAS 2050, the Greenhouse Gas Protocol, EN15804, PEF Guide and guidance documents for EPDs and PCRs, are reviewed according to these criteria.

Results and discussion

None of the investigated guidelines fully follows the systematic framework for allocation. Often, different approaches are recommended for co-products and recycled materials, although the boundary between these flows is not always clear. Many guidelines do not recognize the existence of different LCA goals; therefore, elements of attributional and consequential LCAs are often mixed. The market situation of the recycled material is not always taken into account, e.g. in the mandatory 50/50 method of the PEF Guide. The ILCD Handbook and the General Programme Instructions for the International EPD® System provide most consistent guidance. We argue that consistency does not require a one-formula-fits-all method, as this would favour some product categories and only responds to a certain LCA goal.

Conclusions and perspectives

A critical review of guidelines against a systematic framework for allocation of co-products and recycled materials shows that few guidelines propose a consistent allocation approach. The main obstacles for consistency are the different approaches for co-production and (different types of open-loop) recycling and disregarding of different LCA goals and recycled material markets. We recommend to include material specific guidance in Product Category Rules on the determination of market prices, quality determining factors and relevant material properties for different applications.

     

Developing a systematic framework for consistent allocation in LCA

Purpose

Multifunctionality in life-cycle assessment (LCA) is solved with allocation, for which many different procedures are available. Lack of sufficient guidance and difficulties to identify the correct allocation approach cause a large number of combinations of methods to exist in scientific literature. This paper reviews allocation procedures for recycling situations, with the aim to identify a systematic approach to apply allocation.

Methods

Assumptions and definitions for the most important terms related to multifunctionality and recycling in LCA are given. The most relevant allocation procedures are identified from literature. These procedures are expressed in mathematical formulas and schemes and arranged in a systematic framework based on the underlying objectives and assumptions of the procedures.

Results and discussion

If the LCA goal asks for an attributional approach, multifunctionality can be solved by applying system expansion—i.e. including the co-functions in the functional unit—or partitioning. The cut-off approach is a form of partitioning, attributing all the impacts to the functional unit. If the LCA goal asks for a consequential approach, substitution is applied, for which three methods are identified: the end-of-life recycling method and the waste mining method, which are combined in the 50/50 method. We propose to merge these methods in a new formula: the market price-based substitution method. The inclusion of economic values and maintaining a strict separation between attributional and consequential LCA are considered to increase realism and consistency of the LCA method.

Conclusions and perspectives

We identified the most pertinent allocation procedures—for recycling as well as co-production and energy recovery—and expressed them in mathematical formulas and schemes. Based on the underlying objectives of the allocation procedures, we positioned them in a systematic and consistent framework, relating the procedures to the LCA goal definition and an attributional or consequential approach. We identified a new substitution method that replaces the three existing methods in consequential LCA. Further research should test the validity of the systematic framework and the market price-based substitution method by means of case studies.

     

Selective inhibition of prostaglandin biosynthesis by gold salts and phenylbutazone

Gold salts and phenylbutazone selectively inhibit the synthesis of PGF_2α and PGE₂ respectively. Lowered production of one prostaglandin species is accompanied by an increased production of the other. Selective inhibition by these drugs was observed in the presence of adrenaline, reduced glutathione and copper sulphate under conditions when most anti-inflammatory compounds inhibited PGE₂ and PGF_2α syntheses equally. It is postulated that selective inhibitors may have a different mode of action in vivo and beneficial effects may be related to the endogenous ratio of PGE to PGF required for normal function.     

Sublethal damage during cryopreservation of rainbow trout sperm

Although cellular damage during cryopreservation of freshwater fish spermatozoa has been reported in several studies, there is a lack of correlation between this damage and the fertility rates of eggs using postthawed milt. The apparent lack of such correlation may be due to other undetected sublethal cryodamage, which could affect the cell functionality and viability. This may be extremely important for freshwater fish spermatozoa whose ability to fertilize the egg requires dilution in water or hypoosmotic solutions, an hazardous environment for the cells. This study tested the change in cell permeability during cryopreservation, using Hoechst 33258 to assess cell permeability. The permeability of spermatozoa at different times after dilution in several hypoosmotic media were investigated. In the first trial, fresh semen, sperm diluted in freezing media (CPT), and freeze/thawed semen were studied. Three CPT were tested (Me2SO, DMA, and methanol). In the second trial, the addition of egg yolk as a membrane stabilizer was investigated. Samples were frozen at -20 degreesC/min in a programmable cooler and thawed in a 25 degreesC water bath. Dilution in the CPTs slightly increased the susceptibility of cells to damage but freezing/thawing caused a dramatic increase in the fragility of cells, which were killed in a few seconds after their contact with the hypoosmotic solutions. Egg yolk provided a significant protection to the membrane, allowing the cells a greater and more prolonged survival in the fertilization media. Samples frozen with Me2SO displayed the best results. These results are consistent with the achieved fertility rates that demonstrated sublethal cryodamage in the function of the sperm membrane that was not detected by standard procedures. Copyright 1998 Academic Press.     

Choosing and using diversity indices: insights for ecological applications from the German Biodiversity Exploratories

Biodiversity, a multidimensional property of natural systems, is difficult to quantify partly because of the multitude of indices proposed for this purpose. Indices aim to describe general properties of communities that allow us to compare different regions, taxa, and trophic levels. Therefore, they are of fundamental importance for environmental monitoring and conservation, although there is no consensus about which indices are more appropriate and informative. We tested several common diversity indices in a range of simple to complex statistical analyses in order to determine whether some were better suited for certain analyses than others. We used data collected around the focal plant Plantago lanceolata on 60 temperate grassland plots embedded in an agricultural landscape to explore relationships between the common diversity indices of species richness (S), Shannon's diversity (H'), Simpson's diversity (D₁), Simpson's dominance (D₂), Simpson's evenness (E), and Berger–Parker dominance (BP). We calculated each of these indices for herbaceous plants, arbuscular mycorrhizal fungi, aboveground arthropods, belowground insect larvae, and P. lanceolata molecular and chemical diversity. Including these trait‐based measures of diversity allowed us to test whether or not they behaved similarly to the better studied species diversity. We used path analysis to determine whether compound indices detected more relationships between diversities of different organisms and traits than more basic indices. In the path models, more paths were significant when using H', even though all models except that with E were equally reliable. This demonstrates that while common diversity indices may appear interchangeable in simple analyses, when considering complex interactions, the choice of index can profoundly alter the interpretation of results. Data mining in order to identify the index producing the most significant results should be avoided, but simultaneously considering analyses using multiple indices can provide greater insight into the interactions in a system.     

Horizontal migration of click beetle (Agriotes spp.) larvae depends on food availability

It is generally thought that soil animals face specific foraging conditions because movement through soil is highly energy consuming. The hypothesis tested here is that root‐feeding click beetle larvae (wireworms, Agriotes spp.; Coleoptera: Elateridae), to minimize energy loss, only migrate horizontally through soil when located in food‐depleted surroundings. Larvae were placed at either end of a root density gradient created by the grass Holcus lanatus L. (Poaceae), and their position in the gradient was recorded after 12 days of migration. Larval migration depended on the food situation at the starting point, with larvae moving from food‐depleted to food‐rich areas, but not leaving food‐rich areas. Larvae spread further around food‐rich areas when together with conspecifics than when being alone, presumably to avoid cannibalism. Food density‐dependent migration may have to be taken into account when using trap‐crops to control Agriotes larvae. Success may depend on the timing of trap‐crop establishment relative to the target crop to generate an effective food gradient.     

Cytoplasmic gamma-actin is not required for skeletal muscle development but its absence leads to a progressive myopathy

Nonmuscle gamma(cyto)-actin is expressed at very low levels in skeletal muscle but uniquely localizes to costameres, the cytoskeletal networks that couple peripheral myofibrils to the sarcolemma. We generated and analyzed skeletal muscle-specific gamma(cyto)-actin knockout (Actg1-msKO) mice. Although muscle development proceeded normally, Actg1-msKO mice presented with overt muscle weakness accompanied by a progressive pattern of muscle fiber necrosis/regeneration. Functional deficits in whole-body tension and isometric twitch force were observed, consistent with defects in the connectivity between muscle fibers and/or myofibrils or at the myotendinous junctions. Surprisingly, gamma(cyto)-actin-deficient muscle did not demonstrate the fibrosis, inflammation, and membrane damage typical of several muscular dystrophies but rather presented with a novel progressive myopathy. Together, our data demonstrate an important role for minimally abundant but strategically localized gamma(cyto)-actin in adult skeletal muscle and describe a new mouse model to study the in vivo relevance of subcellular actin isoform sorting.     

Mineral resources in life cycle impact assessment—part I: a critical review of existing methods

Purpose

The safeguard subject of the Area of Protection “natural Resources,” particularly regarding mineral resources, has long been debated. Consequently, a variety of life cycle impact assessment methods based on different concepts are available. The Life Cycle Initiative, hosted by the UN Environment, established an expert task force on “Mineral Resources” to review existing methods (this article) and provide guidance for application-dependent use of the methods and recommendations for further methodological development (Berger et al. in Int J Life Cycle Assess, 2020).

Methods

Starting in 2017, the task force developed a white paper, which served as its main input to a SETAC Pellston Workshop® in June 2018, in which a sub-group of the task force members developed recommendations for assessing impacts of mineral resource use in LCA. This article, based mainly on the white paper and pre-workshop discussions, presents a thorough review of 27 different life cycle impact assessment methods for mineral resource use in the “natural resources” area of protection. The methods are categorized according to their basic impact mechanisms, described and compared, and assessed against a comprehensive set of criteria.

Results and discussion

Four method categories have been identified and their underlying concepts are described based on existing literature: depletion methods, future efforts methods, thermodynamic accounting methods, and supply risk methods. While we consider depletion and future efforts methods more “traditional” life cycle impact assessment methods, thermodynamic accounting and supply risk methods are rather providing complementary information. Within each method category, differences between methods are discussed in detail, which allows for further sub-categorization and better understanding of what the methods actually assess.

Conclusions

We provide a thorough review of existing life cycle impact assessment methods addressing impacts of mineral resource use, covering a broad overview of basic impact mechanisms to a detailed discussion of method-specific modeling. This supports a better understanding of what the methods actually assess and highlights their strengths and limitations. Building on these insights, Berger et al. (Int J Life Cycle Assess, 2020) provide recommendations for application-dependent use of the methods, along with recommendations for further methodological development.