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61.
62.
The construction of a genetic linkage map and evaluation of population genetic diversity both require large numbers of polymorphic
molecular markers. In the present study, we report 31 polymorphic microsatellite markers, of which 28 were isolated from two
repeat-enriched libraries constructed from genomic DNA, and three were detected in two genes (MCH-R1 and MCH-R2) of barfin
flounder (Verasper moseri). A total of 94 alleles were detected with an average of 3.0 alleles per locus. The number of alleles, observed and expected
heterozygosity per locus ranged from two to six, from 0.30 to 1.00 and from 0.33 to 0.78, respectively. Three loci significantly
deviated from Hardy-Weinberg equilibrium after Bonferroni correction (P < 0.0016) and no significant linkage disequilibrum between pairs of loci was found. Cross-species amplification of these markers
was evaluated using the closely related species spotted halibut (Verasper variegatus). This study will potentially be useful for stock management, constructing of a genetic linkage map, mapping economically
important quantitative trait loci (QTL), and studying the population genetic diversity of barfin flounder (Verasper moseri). 相似文献
63.
Genbo Xu Changwei Shao Xiaolin Liao Yongsheng Tian Songlin Chen 《Conservation Genetics》2009,10(4):1009-1011
We describe the first isolation of 12 polymorphic microsatellite markers from Belenger’s jewfish (Johnius belengnerii Cuvier 1830). From a (GT)n-enriched genomic library, 54 microsatellites were selected for designing microsatellite primers,
of which 36 gave working primer pairs. 12 of these loci were polymorphic in a test population of 21 individuals with alleles
ranging from 3 to 18, and expected and observed heterozygosities from 0.5772 to 0.9449 and from 0.4286 to 0.9231, respectively.
No significant linkage disequilibrium between pairs of loci was found, however, loci Jobe24 significantly deviated from Hardy–Weinberg
equilibrium after Bonferroni correction. These polymorphic microsatellite loci should provide sufficient level of genetic
diversity to investigate population structure in Belenger’s jewfish. 相似文献
64.
Guidong Miao Changwei Shao Hongyu Ma Xiaolin Liao Songlin Chen 《Conservation Genetics》2009,10(3):631-633
Starry flounder (Platichthys stellatus) is a rare fish species in China. Here, we reported 12 polymorphic microsatellite loci isolated from a dinucleotide-enriched
genomic library of starry flounder (P. stellatus). The number of alleles, observed and expected heterozygosity per locus in 30 individuals ranged from two to six, from 0.2500
to 1.0000 and from 0.4512 to 0.7667, respectively. One locus significantly deviated from Hardy–Weinberg equilibrium after
Bonferroni correction and no significant linkage disequilibrium between pairs of loci was found. Cross-species amplification
of these microsatellite loci in additional three fish species was performed. These polymorphic microsatellite loci would be
useful for investigating genetic population structure and construction of genetic linkage map in P. stellatus.
Guidong Miao and Changwei Shao have contributed equally. 相似文献
65.
小白蛋白(parvaibumin PV)是一种水溶性低分子量的钙结合蛋白,本实验用免疫疫组化法研究了新生儿大脑皮质含 PV 神经元的分布。PV 阳性神经元为非锥体型,主要分布于中央后回、听皮质及视皮质(17区),在中央前回,wernicke's 区与海马 PV 阳性神经元的数目较少,前额叶很少见到 PV 阳性神经元。在听皮质及中央后回,阳性神经元主要集中于Ⅳ层,少数在Ⅴ、Ⅵ层;在视皮质则分布在Ⅴ、Ⅵ、层及ⅣB 层;wernieke's 区深层(Ⅴ、Ⅵ层)及海马锥体细胞层可见部分阳性神经元。在上述皮质区第Ⅰ层亦可观察到阳性纤维与散在阳性神经元.此外,在中央后回及听皮质的白质内有许多 PV 阳性纤维和终末样结构. 相似文献
66.
Songlin Zhou Cheng Wu Qingsheng Tang Xunrong Zhou Bin He Pingan Chang Xiaozhou He 《Journal of biochemical and molecular toxicology》2023,37(7):e23370
Accumulating evidence has demonstrated the key role of long noncoding (lnc)RNAs in tumorigenesis. Prostate cancer (PCa) is a cancer with high mortality that requires further exploration of the underlying molecular mechanisms. In the present study, we aimed to discover novel potential biomarkers for diagnosing PCa and targeting treatment. Overexpression of the lncRNA, LINC00491, was verified in PCa tumor tissues and cell lines using the real-time polymerase chain reaction. Cell proliferation and invasion were then analyzed via the Cell Counting Kit-8, colony formation, and transwell assays in vitro, and tumor growth in vivo. The interaction of miR-384 with LINC00491, as well as TRIM44, was investigated via bioinformatics analyses, subcellular fractionation, luciferase reporter gene assays, radioimmunoprecipitation, pull-down, and western blot analyses. LINC00491 was overexpressed in PCa tissues and cell lines. LINC00491 knockdown resulted in impaired cell proliferation and invasion in vitro and decreased tumor growth in vivo. Moreover, LINC00491 acted as a sponge for miR-384 and its downstream target, TRIM44. Additionally, miR-384 expression was downregulated in PCa tissues and cell lines, and its expression was negatively correlated with LINC00491. A miR-384 inhibitor restored the inhibitory effects of LINC00491 silencing on PCa cell proliferation and invasion. LINC00491 is a tumor promoter in PCa via enhancing TRIM44 expression by sponging miR-384 to facilitate the development of PCa. LINC00491 plays a significant role in PCa and could serve as both a biomarker for early diagnosis and a novel treatment target. 相似文献
67.
Embryonic stem (ES) cells provide a unique tool for introducing random or targeted genetic alterations, because it is possible that the desired, but extremely rare recombinant genotypes can be screened by drug selection. ES cell-mediated transgenesis has so far been limited to the mouse. In the fish medaka (Oryzias latipes) several ES cell lines have been made available. Here we report the optimized conditions for gene transfer and drug selection in the medaka ES cell line MES1 as a prelude for gene targeting in fish. MES1 cells gave rise to a moderate to high transfection efficiency by the calcium phosphate co-precipitation (5%), commercial reagents Fugene (11%), GeneJuice (21%) and electroporation (>30%). Transient gene transfer and CAT reporter assay revealed that several enhancers/promoters and their combinations including CMV, RSV and ST (the SV40 virus early gene enhancer linked to the thymidine kinase promoter) were suitable regulatory sequences to drive transgene expression in the MES1 cells. We show that neo, hyg or pac conferred resistance to G418, hygromycin or puromycin for positive selection, while the HSV-tk generated sensitivity to ganciclovir for negative selection. The positive-negative selection procedure that is widely used for gene targeting in mouse ES cells was found to be effective also in MES1 cells. Importantly, we demonstrate that MES1 cells after gene transfer and long-term drug selection retained the developmental pluripotency, as they were able to undergo induced differentiation in vitro and to contribute to various tissues and organs during chimeric embryogenesis. 相似文献
68.
Cher Hon Lau Songlin Liu Donald R. Paul Jianzhong Xia Yan‐Ching Jean Hongmin Chen Lu Shao Tai‐Shung Chung 《Liver Transplantation》2011,1(4):634-642
An effective separation of CO2 from H2 can be achieved using currently known polyethylene oxide (PEO)‐based membranes at low temperatures but the CO2 permeability is inadequate for commerical operations. For commercial‐scale CO2/H2 separation, CO2 permeability of these membranes must be significantly enhanced without compromising CO2/H2 selectivity. We report here exceptional CO2/H2 separation properties of a nanohybrid membrane comprising polyethylene glycol methacrylate (PEGMA) grafts on an organic‐inorganic membrane (OIM) consisting of a low molecular weight polypropylene oxide (PPO)‐PEO‐PPO diamine and 3‐glycidyloxypropyltrimethoxysilane (GOTMS), an alkoxysilane. The CO2 gas permeability of this nanohybrid membrane can reach 1990 Barrer with a CO2/H2 selectivity of 11 at 35 °C for a mixed gas mixture comprising 50% CO2 ‐ 50% H2 at 3.5 atm. The transformation of the inorganic silica phase from a well‐dispersed network of finely defined nanoparticles to rough porous clusters appears to be responsible for this OIM membrane exceeding the performance of other state‐of‐the‐art PEO‐based membranes. 相似文献
69.
Qiao S Jiang Z Tian X Wang R Xing G Wan B Bao D Liu Y Hao H Guo J Zhang G 《PloS one》2011,6(12):e28721
Antibody-dependent enhancement (ADE) of virus infection caused by the uptake of virus-antibody complexes by FcγRs is a significant obstacle to the development of effective vaccines to control certain human and animal viral diseases. The activation FcγRs, including FcγRI and FcγRIIa have been shown to mediate ADE infection of virus. In the present paper, we showed that pocine FcγRIIb, an inhibitory FcγR, mediates ADE of PRRSV infection. Stable Marc-145 cell lines expressing poFcγRIIb (Marc-poFcγRII) were established. The relative yield of progeny virus was significantly increased in the presence of sub-neutralization anti-PRRSV antibody. The Fab fragment and normal porcine sera had no effect. Anti-poFcγRII antibody inhibited the enhancement of infection when cells were infected in the presence of anti-PRRSV antibody, but not when cells were infected in the absence of antibody. These results indicate that enhancement of infection in these cells by anti-PRRSV virus antibody is FcγRII-mediated. Identification of the inhibitory FcγR mediating ADE infection should expand our understanding of the mechanisms of pathogenesis for a broad range of infectious diseases and may open many approaches for improvements to the treatment and prevention of such diseases. 相似文献
70.
Danchen Wang Chaochao Ma Yutong Zou Songlin Yu Honglei Li Xinqi Cheng Ling Qiu Tengda Xu 《Journal of Medical Biochemistry》2020,39(3):384
BackgroundIndirect sampling methods are not only inexpensive but also efficient for establishing reference intervals (RIs) using clinical data. This study was conducted to select fully normal records to establish ageand gender-specific RIs for common biochemical analytes by laboratory data mining.MethodsIn total, 280,206 records from 2014 to 2018 were obtained from Peking Union Medical College Hospital. Common biochemical analytes total protein, albumin, total bilirubin (TBil), direct bilirubin (DBil), alanine aminotransferase (ALT), glutamyltranspeptidase (GGT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), potassium, sodium, chlorine, calcium, urea, glucose, uric acid (UA), inorganic phosphorus, creatinine (Cr), total cholesterol, triglyceride, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol] were measured using an automatic analyzer. Sources of variation were identified by multiple regression analysis. The 2.5th and 97.5th percentiles were calculated as the lower and upper limits of the RIs, respectivelyResultsGender was the major source of variation among the 13 common biochemical analytes with an rp > 0.15. In contrast to the value listed in the WS/T 404, nearly all RIs established in this study were significantly narrower. Furthermore, age-specific RIs should be determined for DBil, LDH, and urea, whereas gender-specific RIs are suggested for GGT, LDH, and urea.ConclusionsWe recommend that gender-specific RIs should be established for ALT, AST, GGT, DBil, TBil, UA, and Cr as well as genderand age-specific RIs for urea and ALP. Through indirect sampling, ageand gender-specific RIs for common biochemical analytes were established and analyzed. 相似文献