全文获取类型
收费全文 | 96篇 |
免费 | 22篇 |
出版年
2022年 | 1篇 |
2021年 | 1篇 |
2018年 | 2篇 |
2017年 | 1篇 |
2016年 | 4篇 |
2015年 | 3篇 |
2014年 | 2篇 |
2013年 | 4篇 |
2012年 | 6篇 |
2011年 | 7篇 |
2010年 | 7篇 |
2009年 | 6篇 |
2008年 | 1篇 |
2007年 | 3篇 |
2006年 | 6篇 |
2005年 | 2篇 |
2004年 | 4篇 |
2003年 | 5篇 |
2002年 | 1篇 |
2000年 | 3篇 |
1999年 | 8篇 |
1998年 | 4篇 |
1997年 | 1篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1979年 | 1篇 |
1978年 | 4篇 |
1977年 | 1篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1973年 | 2篇 |
1971年 | 3篇 |
1970年 | 2篇 |
1969年 | 1篇 |
1967年 | 2篇 |
1966年 | 1篇 |
1963年 | 1篇 |
排序方式: 共有118条查询结果,搜索用时 156 毫秒
61.
62.
Targeted mutagenesis of the phospholipase D gene results in decreased virulence of Corynebacterium pseudotuberculosis 总被引:2,自引:1,他引:1
The chromosomal gene encoding the phospholipase D from Corynebacterium pseudotuberculosis (biovar ovis) isolate Whetten 1 was replaced with an allele containing a nonsense mutation. The virulence of the mutant strain (W1.31r1) and the isogenic parental strain were then compared by inoculation of goats. The with-type strain caused abscessation at the site of infection, which then spread to the regional lymph node, while W1.31r1 had a reduced ability to establish a primary infection and was incapable of dissemination. Our results confirm that phospholipase D is a virulence determinant of C. pseudotuberculosis that increases the persistence and spread of the bacteria within the host. 相似文献
63.
64.
Stabler RA Dawson LF Valiente E Cairns MD Martin MJ Donahue EH Riley TV Songer JG Kuijper EJ Dingle KE Wren BW 《PloS one》2012,7(3):e31559
Clostridium difficile has emerged rapidly as the leading cause of antibiotic-associated diarrheal disease, with the temporal and geographical appearance of dominant PCR ribotypes such as 017, 027 and 078. Despite this continued threat, we have a poor understanding of how or why particular variants emerge and the sources of strains that dominate different human populations. We have undertaken a breadth genotyping study using multilocus sequence typing (MLST) analysis of 385 C. difficile strains from diverse sources by host (human, animal and food), geographical locations (North America, Europe and Australia) and PCR ribotypes. Results identified 18 novel sequence types (STs) and 3 new allele sequences and confirmed the presence of five distinct clonal lineages generally associated with outbreaks of C. difficile infection in humans. Strains of animal and food origin were found of both ST-1 and ST-11 that are frequently associated with human disease. An in depth MLST analysis of the evolutionary distant ST-11/PCR ribotype 078 clonal lineage revealed that ST-11 can be found in alternative but closely related PCR ribotypes and PCR ribotype 078 alleles contain mutations generating novel STs. PCR ribotype 027 and 017 lineages may consist of two divergent subclades. Furthermore evidence of microdiversity was present within the heterogeneous clade 1. This study helps to define the evolutionary origin of dominant C. difficile lineages and demonstrates that C. difficile is continuing to evolve in concert with human activity. 相似文献
65.
66.
Cécile Duclairoir Poc Anne Groboillot Olivier Lesouhaitier Jean-Paul Morin Nicole Orange Marc JG Feuilloley 《BMC research notes》2011,4(1):503
Background
Low environmental air quality is a significant cause of mortality and morbidity and this question is now emerging as a main concern of governmental authorities. Airborne pollution results from the combination of chemicals, fine particles, and micro-organisms quantitatively or qualitatively dangerous for health or for the environment. Increasing regulations and limitations for outdoor air quality have been decreed in regards to chemicals and particles contrary to micro-organisms. Indeed, pertinent and reliable tests to evaluate this biohazard are scarce. In this work, our purpose was to evaluate the Caenorhaditis elegans killing test, a model considered as an equivalent to the mouse acute toxicity test in pharmaceutical industry, in order to monitor air bacterial quality.Findings
The present study investigates the bacterial population in dust clouds generated during crop ship loading in harbor installations (Rouen harbor, Normandy, France). With a biocollector, airborne bacteria were impacted onto the surface of agar medium. After incubation, a replicate of the colonies on a fresh agar medium was done using a velvet. All the replicated colonies were pooled creating the "Total Air Sample". Meanwhile, all the colonies on the original plate were isolated. Among which, five representative bacterial strains were chosen. The virulence of these representatives was compared to that of the "Total Air Sample" using the Caenorhaditis elegans killing test. The survival kinetic of nematodes fed with the "Total Air Sample" is consistent with the kinetics obtained using the five different representatives strains.Conclusions
Bacterial air quality can now be monitored in a one shot test using the Caenorhaditis elegans killing test.67.
Jacques-Henri Torres Michael Mechali Olivier Romieu Paul Tramini Sylvie Callas Frédéric JG Cuisinier Bernard Levallois 《Biomedical engineering online》2011,10(1):28
Background
Most dental implant systems are presently made of two pieces: the implant itself and the abutment. The connection tightness between those two pieces is a key point to prevent bacterial proliferation, tissue inflammation and bone loss. The leak has been previously estimated by microbial, color tracer and endotoxin percolation. 相似文献68.
69.
Comparative phylogenomics of Clostridium difficile reveals clade specificity and microevolution of hypervirulent strains 下载免费PDF全文
Stabler RA Gerding DN Songer JG Drudy D Brazier JS Trinh HT Witney AA Hinds J Wren BW 《Journal of bacteriology》2006,188(20):7297-7305
Clostridium difficile is the most frequent cause of nosocomial diarrhea worldwide, and recent reports suggested the emergence of a hypervirulent strain in North America and Europe. In this study, we applied comparative phylogenomics (whole-genome comparisons using DNA microarrays combined with Bayesian phylogenies) to model the phylogeny of C. difficile, including 75 diverse isolates comprising hypervirulent, toxin-variable, and animal strains. The analysis identified four distinct statistically supported clusters comprising a hypervirulent clade, a toxin A(-) B(+) clade, and two clades with human and animal isolates. Genetic differences among clades revealed several genetic islands relating to virulence and niche adaptation, including antibiotic resistance, motility, adhesion, and enteric metabolism. Only 19.7% of genes were shared by all strains, confirming that this enteric species readily undergoes genetic exchange. This study has provided insight into the possible origins of C. difficile and its evolution that may have implications in disease control strategies. 相似文献
70.
Marlies van Nimwegen Arlène D Speelman Katrijn Smulders Sebastiaan Overeem George F Borm Frank JG Backx Bastiaan R Bloem Marten Munneke ParkFit study group 《BMC neurology》2010,10(1):70