全文获取类型
收费全文 | 465篇 |
免费 | 21篇 |
出版年
2022年 | 3篇 |
2021年 | 3篇 |
2018年 | 3篇 |
2017年 | 4篇 |
2015年 | 9篇 |
2014年 | 8篇 |
2013年 | 20篇 |
2012年 | 22篇 |
2011年 | 17篇 |
2010年 | 11篇 |
2009年 | 16篇 |
2008年 | 20篇 |
2007年 | 17篇 |
2006年 | 12篇 |
2005年 | 19篇 |
2004年 | 20篇 |
2003年 | 20篇 |
2002年 | 23篇 |
2001年 | 20篇 |
2000年 | 25篇 |
1999年 | 13篇 |
1998年 | 11篇 |
1997年 | 4篇 |
1996年 | 8篇 |
1995年 | 4篇 |
1994年 | 10篇 |
1993年 | 3篇 |
1992年 | 9篇 |
1991年 | 13篇 |
1990年 | 12篇 |
1989年 | 14篇 |
1988年 | 11篇 |
1987年 | 12篇 |
1986年 | 11篇 |
1985年 | 3篇 |
1984年 | 9篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1981年 | 2篇 |
1979年 | 5篇 |
1978年 | 3篇 |
1977年 | 9篇 |
1976年 | 5篇 |
1975年 | 5篇 |
1974年 | 3篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1916年 | 1篇 |
排序方式: 共有486条查询结果,搜索用时 15 毫秒
481.
A caa3-type terminal cytochrome c oxidase (EC 1.9.3.1) from the thermophilic bacterium PS3 containing three subunits showed conversion from resting into pulsed form. Upon pulsing (reduction and re-oxidation), the cytochrome c oxidase activity increased over 10-fold. This enhanced activity of the pulsed enzyme gradually decayed. Addition of phospholipids, necessary for the enzyme activity, did not affect this decay process. Small changes in the absorption spectrum were observed for the resting-into-pulsed transition and for H2O2 ligation to the pulsed enzyme. The e.p.r. spectrum of the resting enzyme was very similar to that of mitochondrial enzyme, but the transient g = 5, 1.78 and 1.69 set of e.p.r. signals, associated with the pulsed bovine heart oxidase, were not observed in the case of pulsed bacterium-PS3 enzyme. 相似文献
482.
M Yoshida N Sone H Hirata Y Kagawa 《Biochemical and biophysical research communications》1978,84(1):117-122
The numbers of sulfhydryl residues in F1-ATPase of thermophilic bacterium PS3 and its isolated subunits were analyzed with Ellman's reagent. This F1-ATPase contained three sulfhydryl residues and no disulfide bridge. Of the five kinds of subunits of the F1-ATPase, only the α subunit contained one sulfhydryl residue. So there are three α subunits in one molecule of the F1-ATPase. 相似文献
483.
484.
Carbon monoxide-binding cytochromes in the respiratory chain of the thermophilic bacterium PS3 grown with sufficient or limited aeration 总被引:1,自引:0,他引:1
The effects of aeration on the growth and cytochrome patterns of thermophilic bacterium PS3 were studied; bacteria grown with strong aeration synthesized cytochromes c, b, and aa3, while those grown with low aeration, showing non-exponential growth, synthesized higher amounts of cytochromes c and b including o, and a lower amount of cytochrome a (a3). The CO-difference spectra indicated that the terminal oxidase was cytochrome aa3 for high aeration conditions and the cytochrome o for low aeration conditions. Cytochrome o can be solubilized by Triton X-100 from the membrane fraction of bacteria grown under oxygen-limited conditions. The carbon monoxide complex of cytochrome o, obtained by exposing this extract to CO, was photolyzed and the subsequent rebinding of CO was analyzed; it followed first order kinetics with a rate constant of around 8 s-1 at 25 degrees C. At liquid nitrogen temperature, CO-rebinding did not occur. The CO-difference spectrum of purified cytochrome oxidase sample from the bacteria grown with strong aeration (Sone, N., et al. (1979) FEBS Lett. 106, 39-42) revealed the presence of a small amount of a cytochrome o-like pigment besides cytochrome aa3. Analysis of the CO complexes of these chromophores showed rate constants of 29-30 s-1 for cytochrome aa3 and 35-42 s-1 for the o-like pigment, indicating that the cytochrome o-like pigment contaminating the purified cytochrome oxidase preparation was not typical cytochrome o. 相似文献
485.
486.