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PCR primed with minisatellite core sequences yields DNA fingerprinting probes in wheat 总被引:1,自引:0,他引:1
P. J. Bebeli Z. Zhou D. J. Somers J. P. Gustafson 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(1-2):276-283
Four minisatellite core sequences were used as primers in a polymerase chain reaction (PCR) technique, known as the directed
amplification of minisatellite-region DNA (DAMD), to detect polymorphisms in three pairs of hexaploid/tetraploid wheat cultivars.
In each pair, the tetraploid cultivar (genomic formula AABB) was extracted from its corresponding hexaploid (genomic formula
AABBDD) parent. Reproducible profiles of the amplified products revealed characteristic bands that were present only in the
hexaploid wheats but not in their extracted tetraploids. Some polymorphisms were observed among the hexaploid cultivars. Twenty-three
DAMD-PCR amplified fragments were isolated and screened as molecular probes on the genomic DNA of wild wheat species, hexaploid
wheat and triticale cultivars. Subsequently, 8 of the fragments were cloned and sequenced. The DAMD-PCR clones revealed various
degrees of polymorphism among different wild and cultivated wheats. Two clones yielded individual-specific DNA fingerprinting
patterns which could be used for species differentiation and cultivar identification. The results demonstrated the use of
DAMD-PCR as a tool for the isolation of informative molecular probes for DNA fingerprinting in wheat cultivars and species.
Received: 13 May 1996/Accepted: 11 October 1996 相似文献
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Thermal stability and pH optima of NADH-nitrate reductase-associated cytochrome c reductase and FMNH2-nitrate reductase from wild type, cv Steptoe or Winer, and mutants nar 1d, nar 1g, nar 1h, Xno 18 and Xno 19 were compared to determine if structural differences in the nitrate reductase protein could be detected. Also, the nitrate reductase-associated cytochrome c reductase from nar 1d was purified and compared with the wild type by peptide mapping. The pH optimum for FMNH2-nitrate reductase from Steptoe and nar 1h, and for NADH-cytochrome c reductase from Steptoe, nar 1d, nar 1g and nar 2a was 7.5. Thermal stabilities of the nitrate reductase-associated activities (FMNH2-nitrate reductase or NADH-cytochrome c reductase) from nar mutants were less than the Steptoe wild type, while Xno mutants were equal to the Winer wild type. Cleveland peptide maps of nar 1d NADH-cytochrome c reductase and Steptoe nitrate reductase were identicalwhen digested with endoprotease lys-C but were distinctly different in one peptide when digested with Staphylococcus aureus endoprotease V8. These results provide evidence that nar 1 gene codes for the nitrate reductase polypeptide. 相似文献
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Yi-Huang Hsueh Eileen B. Somers Didier Lereclus Amy C. Lee Wong 《Applied microbiology》2006,72(7):5089-5092
The ΔplcR mutant of Bacillus cereus strain ATCC 14579 developed significantly more biofilm than the wild type and produced increased amounts of biosurfactant. Biosurfactant production is required for biofilm formation and may be directly or indirectly repressed by PlcR, a pleiotropic regulator. Coating polystyrene plates with surfactin, a biosurfactant from Bacillus subtilis, rescued the deficiency in biofilm formation by the wild type. 相似文献
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Marion R. Steiner Ken Somers Sheldon Steiner 《Biochemical and biophysical research communications》1974,61(2):795-801
[14C]-2 deoxy-D-glucose is incorporated into the glycolipids of both normal and transformed cells. The chromatographic pattems of [14C]-2 deoxy-D-glucose labeled lipids differ markedly in oncornavirus and herpes simplex virus-transformed cells as compared to normal and virus-infected but not transformed cells. Deoxyglucoselabeled lipids with intermediate chromatographic mobility were enriched in normal and virus-infected but not transformed cells. Studies with a murine sarcoma virus-infected cell line which is temperature-sensitive for transformation indicated that the altered chromatographic pattern of [14C]-2 deoxy-D-glucose labeled lipids was related to the expression of the transformed phenotype. 相似文献
110.
An automated system is described for the hypoiodite oxidation of aldoses and substituted aldoses to the corresponding aldonic acids. Automated determination of the glyoxylic acid and formaldehyde obtained on oxidation with periodate enables the 3-O-, 4-O-, and 6-O-substituted aldonic acids to be distinguished. The method is applied to the analysis of oligosaccharides in column eluates. 相似文献