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151.
Rieta Gols Nicole M. Van Dam Ciska E. Raaijmakers Marcel Dicke Jeffrey A. Harvey 《Oikos》2009,118(5):733-742
In recent years, increasing attention has been paid in exploring the role of direct plant defence, through the production of allelochemicals, on the performance of parasitoid wasps and their hosts. However, few studies have determined if parasitoids can detect differences in plant quality and thus preferentially attack hosts on which their progeny develop most successfully. In this study we examined the development and preference of two endoparasitoids, Diadegma semiclausum and Cotesia glomerata , developing in larvae of their respective hosts, Plutella xylostella and Pieris brassicae . In turn, these were reared on different wild populations of black mustard Brassica nigra originating in the Netherlands and Sicily (Italy), as well as single cultivated strains of B. nigra and brown mustard, B. juncea . Chemical analyses of foliar glucosinolates and volatile emissions by P. xylostella -damaged plants revealed large differences between B. nigra and B. juncea plants, with smaller differences among the B. nigra populations. The four mustard populations differentially affected development time and body mass of the herbivores and parasitoids. Contrasts among the means revealed significant differences mainly between B. nigra and B. juncea . Both parasitoids, however, preferred to alight on plants in which their progeny developed most successfully. In behavioural bioassays, D. semiclausum did not discriminate among the B. nigra populations and preferred to alight on B. juncea , which was the best plant population for parasitoid development. By contrast, C. glomerata females exhibited the lowest preference for Italian B. nigra populations, on which adult parasitoid size was the smallest. These results reveal that parasitoids can detect even small differences in plant quality presumably through their volatile blends and that plant preference and offspring performance in the two species are 'optimally synchronized'. 相似文献
152.
153.
Oreopithecus bambolii is one of the few hominoids that evolved under insular conditions, resulting in the development of unique adaptations that have fueled an intensive debate. The palaeoenvironment associated with this great ape has been the subject of great controversy as well. On the one hand, palaeobotanical data indicate that Oreopithecus likely inhabited mixed mesophytic forests interrupted by swamps; on the other hand, an abundance of hypsodont bovids points towards the existence of dry and open environments. Here, we provide a new approach based on the ecomorphology of the extinct endemic Muridae (rats and mice) of the so-called Oreopithecus faunas. Our results show that the successive species of endemic insular murids (Huerzelerimys and Anthracomys) evolved a number of adaptations observed only in extant family members that include significant proportions of grass in their diet. While this fits the pattern exhibited by large mammals, it contrasts with the available palaeobotanical information, which indicates that grasses were minor components of the vegetation. This contradiction may be explained because these endemic murids may have been adapted to the consumption of particular food items such as hard parts of aquatic plants (as shown by some extant murid species). However, because it is unlikely that the remaining herbivore mammals were adapted to this diet as well, we favour an alternative hypothesis that takes into account the peculiar ecological conditions of insular ecosystems leading to a density-dependent selective regime with strong competition. Such a regime would promote the selection of dental adaptations to increase feeding efficiency and durability of the dentition (such as hypsodonty) as seen in some fossil insular ruminants. This hypothesis requires further testing, but may partly account for parallel evolution of dental traits in phylogenetically unrelated insular mammals. 相似文献
154.
The structure of the natural cytotoxicity receptor NKp44, described in this issue of Structure, adds to our rapidly expanding knowledge of the structure of natural killer cell receptors, which play a key role in the elimination of virally infected and tumor cells during innate immune responses. 相似文献
155.
Fernando Segato André R. L. Damásio Rosymar C. de Lucas Fabio M. Squina Rolf A. Prade 《Microbiology and molecular biology reviews》2014,78(4):588-613
SUMMARY
Biomass is constructed of dense recalcitrant polymeric materials: proteins, lignin, and holocellulose, a fraction constituting fibrous cellulose wrapped in hemicellulose-pectin. Bacteria and fungi are abundant in soil and forest floors, actively recycling biomass mainly by extracting sugars from holocellulose degradation. Here we review the genome-wide contents of seven Aspergillus species and unravel hundreds of gene models encoding holocellulose-degrading enzymes. Numerous apparent gene duplications followed functional evolution, grouping similar genes into smaller coherent functional families according to specialized structural features, domain organization, biochemical activity, and genus genome distribution. Aspergilli contain about 37 cellulase gene models, clustered in two mechanistic categories: 27 hydrolyze and 10 oxidize glycosidic bonds. Within the oxidative enzymes, we found two cellobiose dehydrogenases that produce oxygen radicals utilized by eight lytic polysaccharide monooxygenases that oxidize glycosidic linkages, breaking crystalline cellulose chains and making them accessible to hydrolytic enzymes. Among the hydrolases, six cellobiohydrolases with a tunnel-like structural fold embrace single crystalline cellulose chains and cooperate at nonreducing or reducing end termini, splitting off cellobiose. Five endoglucanases group into four structural families and interact randomly and internally with cellulose through an open cleft catalytic domain, and finally, seven extracellular β-glucosidases cleave cellobiose and related oligomers into glucose. Aspergilli contain, on average, 30 hemicellulase and 7 accessory gene models, distributed among 9 distinct functional categories: the backbone-attacking enzymes xylanase, mannosidase, arabinase, and xyloglucanase, the short-side-chain-removing enzymes xylan α-1,2-glucuronidase, arabinofuranosidase, and xylosidase, and the accessory enzymes acetyl xylan and feruloyl esterases. 相似文献156.
Elisabeth Astrup Jeshina Janardhanan Kari Otterdal Thor Ueland John A. J. Prakash Tove Lekva ?ystein A. Strand O. C. Abraham Kurien Thomas Jan Kristian Dam?s Prasad Mathews Dilip Mathai P?l Aukrust George M. Varghese 《PLoS neglected tropical diseases》2014,8(2)
Background
Scrub typhus, caused by Orientia tsutsugamushi, is endemic in the Asia-Pacific region. Mortality is high if untreated, and even with treatment as high as 10–20%, further knowledge of the immune response during scrub typhus is needed. The current study was aimed at comparing plasma levels of a variety of inflammatory mediators in scrub typhus patients and controls in South India in order to map the broader cytokine profile and their relation to disease severity and clinical outcome.Methodology/Principal Findings
We examined plasma levels of several cytokines in scrub typhus patients (n = 129) compared to healthy controls (n = 31) and infectious disease controls (n = 31), both in the acute phase and after recovery, by multiplex technology and enzyme immunoassays. Scrub typhus patients were characterized by marked changes in the cytokine network during the acute phase, differing not only from healthy controls but also from infectious disease controls. While most of the inflammatory markers were raised in scrub typhus, platelet-derived mediators such as RANTES were markedly decreased, probably reflecting enhanced platelet activation. Some of the inflammatory markers, including various chemokines (e.g., interleukin-8, monocyte chemoattractant peptide-1 and macrophage inflammatory protein-1β) and downstream markers of inflammation (e.g., C-reactive protein and pentraxin-3), were also associated with disease severity and mortality during follow-up, with a particular strong association with interleukin-8.Conclusions/Significance
Our findings suggest that scrub typhus is characterized by a certain cytokine profile that includes dysregulated levels of a wide range of mediators, and that this enhanced inflammation could contribute to disease severity and clinical outcome. 相似文献157.
George B. McManus Barbara A. Costas Hans G. Dam Rubens M. Lopes Salvador A. Gaeta Sylvia M. Susini Carol H. Rosetta 《Hydrobiologia》2007,575(1):69-81
We measured grazing by herbivorous zooplankton (<200 μm fraction) in coastal and slope regions of the South Brazil Bight.
Using the dilution technique, we performed nine experiments during the austral summer, when nutrient-rich South Atlantic Central
Water is present on the shelf, and five during winter. These experiments provide the first estimates of microzooplankton grazing
in the western South Atlantic Ocean. Model II regression showed a strong relationship between phytoplankton intrinsic growth
rates and grazing, with a slope of 0.64 (±0.28; 95% confidence interval) indicating that microzooplankton grazing could account
for the majority of phytoplankton mortality. Both phytoplankton growth and microzooplankton grazing were higher during the
summer upwelling season, compared to winter. For the two experiments that were conducted in oligotrophic slope water, grazing
accounted for >80% of phytoplankton production. A comparison of incubations with and without added inorganic nutrients showed
no consistent stimulation of phytoplankton growth (slope of enriched versus unenriched treatments not significantly different
from 1). Estimates from microscopic counts of heterotrophic organisms >10 μm indicated that copepod nauplii comprised the
largest share of the microzooplankton biomass (mean 62.4 ± 5.8% SE). Grazing estimates were not correlated with microzooplankton
biomass, whether or not nauplii were included, suggesting that most of the grazing was done by nano-sized zooplankton.
Electronic Supplementary Material Electronic supplementary material is available in the online version of this article at and is accessible for authorized users.
Handling editor: S. Wellekens 相似文献
158.
Hou SM Van Dam FJ de Zwart F Warnock C Mognato M Turner J Podlutskaja N Podlutsky A Becker R Barnett Y Barnett CR Celotti L Davies M Hüttner E Lambert B Tates AD 《Mutation research》1999,431(2):211-221
The T-cell cloning assay, which enables the enumeration and molecular analysis of 6-thioguanine resistant (HPRT-negative) mutant T-cells, has been extensively used for studying human somatic gene mutation in vivo. However, large inter-laboratory variations in the HPRT mutant frequency (MF) call for further investigation of inter-laboratory differences in the experimental methodology, and development of an optimal but easy uniform cloning protocol. As part of the EU Concerted Action on HPRT Mutation (EUCAHM), we have carried out two Ring tests for the T-cell cloning assay. For each test, duplicate and coded samples from three buffy coats were distributed to five laboratories for determination of MF using six different protocols. The results indicated a good agreement between split samples within each laboratory. However, both the cloning efficiencies (CEs) and MFs measured for the same blood donors showed substantial inter-laboratory variations. Also, different medium compositions used in one and the same laboratory resulted in a remarkable difference in the level of MF. A uniform operating protocol (UOP) was proposed and compared with the traditional protocols in the second Ring test. The UOP (preincubation) increased the CE in laboratories traditionally using preincubation, but decreased the CE in laboratories traditionally using priming. Adjusted for donor, use of different protocols contributed significantly to the overall variation in lnCE (P=0.0004) and lnMF (P=0.03), but there was no significant laboratory effect on the lnCE (P=0.38) or lnMF (P=0.14) produced by the UOP alone. Finally, a simplified version of the UOP using the serum-free medium X-Vivo 10 and PMA was tested in one laboratory, and found to produce a considerable increase in CE. This modified UOP needs to be further evaluated in order to be used for future databases on HPRT MFs in various populations. 相似文献
159.
Leakage-free rapid quenching technique for yeast metabolomics 总被引:2,自引:0,他引:2
André B. Canelas Cor Ras Angela ten Pierick Jan C. van Dam Joseph J. Heijnen Walter M. van Gulik 《Metabolomics : Official journal of the Metabolomic Society》2008,4(3):226-239
Accurate determination of intracellular metabolite levels requires reliable, reproducible techniques for sampling and sample
treatment. Quenching in 60% (v/v) methanol at −40°C is currently the standard method for sub-second arrest of metabolic activity
in microbial metabolomics but there have been contradictory reports in the literature on whether leakage of metabolites from
the cells occurs. We have re-evaluated this method in S. cerevisiae using a comprehensive, strictly quantitative approach. By determining the levels of a large range of metabolites in different
sample fractions and establishing mass balances we could trace their fate during the quenching procedure and confirm that
leakage of metabolites from yeast cells does occur during conventional cold methanol quenching, to such an extent that the
levels of most metabolites have been previously underestimated by at least twofold. In addition, we found that the extent
of leakage depends on the time of exposure, the temperature and the properties of the methanol solutions. Using the mass balance
approach we could study the effect of different quenching conditions and demonstrate that leakage can be entirely prevented
by quenching in pure methanol at ≤−40°C, which we propose as a new improved method. Making use of improved data on intracellular
metabolite levels we also re-evaluated the need of sub-second quenching of metabolic activity and of removing the extracellular
medium. Our findings have serious implications for quantitative metabolomics-based fields such as non-stationary 13C flux analysis, in vivo kinetic modeling and thermodynamic network analysis.
相似文献
André B. CanelasEmail: |
160.