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21.
Differentiation potential of menstrual blood‐ versus bone marrow‐stem cells into glial‐like cells
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Kristin LM Boylan Somaieh Afiuni-Zadeh Melissa A Geller Kayla Hickey Timothy J Griffin Stefan E Pambuccian Amy PN Skubitz 《Clinical proteomics》2014,11(1):30
Background
The proteomic analysis of body fluids is a growing technology for the identification of protein biomarkers of disease. Given that Papanicolaou tests (Pap tests) are routinely performed on over 30 million women annually in the U.S. to screen for cervical cancer, we examined the residual Pap test fluid as a source of protein for analysis by mass spectrometry (MS). In the liquid-based Pap test, cervical cells are collected from the ectocervix and placed into an alcohol-based fixative prior to staining and pathologic examination. We hypothesized that proteins shed by cells of the female genital tract can be detected in the Pap test fixative by MS-based proteomic techniques. We examined the feasibility of using residual fluid from discarded Pap tests with cytologically “normal” results to optimize sample preparation for MS analysis. The protein composition of the cell-free Pap test fluid was determined by silver staining of sodium dodecyl sulfate -polyacrylamide gels, and the abundance of serum proteins was examined by Western immunoblot using an antibody against human serum albumin. Both pooled and individual samples were trypsin digested and analyzed by two-dimensional MS/MS. Proteins were identified by searching against the Human Uniprot database, and characterized for localization, function and relative abundance.Results
The average volume of the residual Pap test fluid was 1.5 ml and the average protein concentration was 0.14 mg/ml. By Western immunoblot we showed that the amount of albumin in each sample was significantly reduced compared to normal serum. By MS/MS, we identified 714 unique proteins in pooled Pap test samples and an average of 431 proteins in individual samples. About 40% of the proteins identified were extracellular or localized to the plasma membrane. Almost 20% of the proteins identified were involved in immunity and defense, characteristic of the healthy cervical-vaginal proteome. By merging the protein sets from the individual and pooled Pap test samples, we created a “Normal Pap test Core Proteome” consisting of 153 proteins.Conclusions
Residual Pap test fluid contains a sufficient amount of protein for analysis by MS and represents a valuable biospecimen source for the identification of protein biomarkers for gynecological diseases. 相似文献23.
Dadkhah A Fatemi F Kazemnejad S Rasmi Y Ashrafi-Helan J Allameh A 《Molecular and cellular biochemistry》2006,281(1-2):145-152
Recently we reported that ferric reducing ability of plasma (FRAP) assay, as an index of total antioxidant activity, increases
in growing rats in response to high dose of vitamin K. In this study, it was found that acetaminophen (APAP) can cause elevation
in FRAP in suckling and adult rats. This study was initiated to assess the contribution of individual antioxidant factors
on elevation in FRAP. A surge in FRAP, 1 h after high dose APAP (250 or 450 mg/kg BW) administration was recorded in both
young as well as adults. Whereas, low dose drug (25 mg/kg) failed to alter FRAP in both the age groups. Time-course studies
show that drug-dependent elevation in FRAP begin rapidly, reaching a maximum at 1 h (> 500%). Increased FRAP was associated
with a marked increase (∼14-fold) in plasma bilirubin, 6 h after drug administration at 450 mg/kg only in suckling rats. Similarly,
APAP-related increase in superoxide dismutase activity in erythrocytes was limited to young rats of both the age groups. Other
factors measured during this period viz., plasma uric acid, bilirubin and total protein together with catalase activity of
erythrocytes remained unchanged in treated rats. Under these circumstances, APAP-related depletion in liver glutathione was
almost similar in both the age groups. During a 12 h study, the concentration of lipid peroxidation products, in liver of
treated groups remained within the levels of respective controls. The endpoint hepatotoxic effects of APAP was almost similar
in both the age groups, suggesting that like adults, immature rats can cope with toxic effects of APAP owing to their drug-dependent
induction in certain antioxidant factors. 相似文献
24.
Hossein Hooshyar Hedayat Rahemi Muhammad Hossein Akhbari Shad Behrooz Khezri 《Journal of molecular modeling》2009,15(5):525-536
The molecular structure (hydrogen bonding, bond distances and angles), dipole moment and vibrational spectroscopic data [vibrational frequencies, IR and vibrational circular dichroism (VCD)] of cyclobutanone?HX (X?=?F, Cl) complexes were calculated using density functional theory (DFT) and second order Møller–Plesset perturbation theory (MP2) with basis sets ranging from 6–311G, 6–311G**, 6–311 + + G**. The theoretical results are discussed mainly in terms of comparisons with available experimental data. For geometric data, good agreement between theory and experiment is obtained for the MP2 and B3LYP levels with basis sets including diffuse functions. Surface potential energy calculations were carried out with scanning HCl and HF near the oxygen atom. The nonlinear hydrogen bonds of 1.81 Å and 175° for HCl and 1.71 Å and 161° for HF were calculated. In these complexes the C=O and H–X bonds participating in the hydrogen bond are elongated, while others bonds are compressed. The calculated vibrational spectra were interpreted and the band assignments reported are in excellent agreement with experimental IR spectra. The C=O stretching vibrational frequencies of the complexes show red shifts with respect to cyclobutanone. 相似文献
25.
Somaieh Zafari Vahid Niknam Rita Musetti Seyyed Nasr Noorbakhsh 《Acta Physiologiae Plantarum》2012,34(2):561-568
The objective of the present work was to study biochemical alterations in lime plants infected by the Candidatus Phytoplasma aurantifoliae. Changes in antioxidant activities, content of chlorophylls (Chl), carotenoids (Car), soluble proteins, sugars and auxin
(IAA) in infected plant were investigated. The activities of polyphenol oxidase (PPO), peroxidase (POX) and superoxide dismutase
(SOD) were observed to be greater in infected leaves than the healthy control. Also according to non-denaturing PAGE, in infected
leaves all the antioxidative enzymes isoforms were stronger than that of the healthy control. These results suggest that antioxidant
enzymes can be activated in response to infection by phytoplasma. The decrease in content of proteins, total soluble and reducing
sugars in infected plants point out changes in host metabolism due to the phytoplasma infection. The reduction in chlorophylls
and auxin content shows that the phytoplasma can interfere in photosynthesis and induces senescence in the leaf. In conclusion,
this study provides new insights into the lime response to phytoplasma infection. 相似文献
26.
Background
We study the role of gonadectomy on the response to unavoidable stress and the role of testosterone replacement on gonadectomy in the male Naval Medical Research Institute mice (30±5 g) were studied. For this purpose, the hormonal and metabolic changes were investigated.Methods
In the experimental group, the gonads were surgically removed, and a cannula was inserted into the left lateral ventricle. For acute and chronic stress induction, animals were placed in the communication box for 30 min for one day and four consecutive days, respectively. The animals received different doses of intraventricular (ICV) testosterone (0.01, 0.05, 0.1 μg/mouse) 5 minutes or intraperitoneal (IP) testosterone (0.05, 0.01, 0.1 mg/kg) 30 minutes before the stress induction.Results
The results showed that acute and chronic stress increases plasma cortisol concentration. IP testosterone injections of testosterone did not decrease cortisol concentrations in response to acute stress, whereas ICV injections did reduce cortisol concentrations. The stress reduced anorexia time, while the administration of testosterone increased anorexia time. In addition, acute stress reduced food intake in the gonadectomized mice. IP testosterone at 0.01 and 0.05 mg/kg increased food intake. Additionally, stress in gonadectomized mice reduced water intake, while the IP injection of testosterone in chronic stress further reduced water intake. Also, stress reduced the animals’ brain/adrenal volumes, while the IP and ICVinjection of testosterone at 0.01 mg/kg inhibited this effect.Conclusion
The results showed that the IP (0.05, 0.01, 0.1 mg/kg) and ICV (0.01, 0.05, 0.1 μg/mouse) administration of testosterone in the gonadectomized mice can modulate hormonal and metabolic changes induced by stress.27.
Gholami Leila Jokar Safura Fatahi Yousef Samandari Hedayat Hamzehalipour Almaki Javad Hosseini Marjan Parviz Mohsen 《Cellular and molecular neurobiology》2022,42(4):1125-1139
Cellular and Molecular Neurobiology - The current study aimed to determine the protective effect of AY9944 related to Caveolin-1 and Claudin-5 role in lipid raft, which can rescue the... 相似文献
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Salehi H Karbalaie K Razavi S Tanhaee S Nematollahi M Sagha M Nasr-Esfahani MH Baharvand H 《The International journal of developmental biology》2011,55(3):321-326
The role of somites and notochords in neuroectoderm differentiation from the embryonic ectoderm and its subsequent patterning into regional compartments along rostro-caudal and dorso-ventral axes, especially in humans, remains elusive. Here, we demonstrate the co-culture effect of somites and notochords isolated from chicken embryos on the neuronal differentiation and regional identity of an adherent culture of human embryonic stem cells (hESCs). Notochord increased the efficiency and speed of neuronal induction, whereas somites had a weak neuronal inducing effect on hESCs. However, a synergistic effect was not observed when notochords and somites were used together. Moreover, in somite and notochord co-culture groups, hESCs-derived neuronal cells expressed HOXB4, OTX2, IRX3 and PAX6, indicative of dorsal hindbrain and ventral anterior identities, respectively. Our results reveal the influence of embryonic notochord and somite co-culture in providing neuronal induction as well as rostro-caudal and dorso-ventral regional identity of hESCs-derived neuronal cells. This study provides a model through which in vivo neuronal induction events may be imitated. 相似文献