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21.
Factors controlling decomposition rates of fine root litter in temperate forests and grasslands 总被引:3,自引:0,他引:3
Emily F. Solly Ingo Schöning Steffen Boch Ellen Kandeler Sven Marhan Beate Michalzik Jörg Müller Jakob Zscheischler Susan E. Trumbore Marion Schrumpf 《Plant and Soil》2014,382(1-2):203-218
Background and aims
Fine root decomposition contributes significantly to element cycling in terrestrial ecosystems. However, studies on root decomposition rates and on the factors that potentially influence them are fewer than those on leaf litter decomposition. To study the effects of region and land use intensity on fine root decomposition, we established a large scale study in three German regions with different climate regimes and soil properties. Methods In 150 forest and 150 grassland sites we deployed litterbags (100 μm mesh size) with standardized litter consisting of fine roots from European beech in forests and from a lowland mesophilous hay meadow in grasslands. In the central study region, we compared decomposition rates of this standardized litter with root litter collected on-site to separate the effect of litter quality from environmental factors.Results
Standardized herbaceous roots in grassland soils decomposed on average significantly faster (24?±?6 % mass loss after 12 months, mean ± SD) than beech roots in forest soils (12?±?4 %; p?0.001). Fine root decomposition varied among the three study regions. Land use intensity, in particular N addition, decreased fine root decomposition in grasslands. The initial lignin:N ratio explained 15 % of the variance in grasslands and 11 % in forests. Soil moisture, soil temperature, and C:N ratios of soils together explained 34 % of the variance of the fine root mass loss in grasslands, and 24 % in forests.Conclusions
Grasslands, which have higher fine root biomass and root turnover compared to forests, also have higher rates of root decomposition. Our results further show that at the regional scale fine root decomposition is influenced by environmental variables such as soil moisture, soil temperature and soil nutrient content. Additional variation is explained by root litter quality. 相似文献22.
Purification and characterization of a protein antigen from Leptospira interrogans serovar hardjo, common to a wide range of bacteria 总被引:1,自引:0,他引:1
A protein with a molecular mass of 64 kDa (P64) from Leptospira interrogans serovar hardjo was partially purified by using successively, phase partitioning with Triton X-114, ion-exchange chromatography and sucrose gradient centrifugation. Purification to homogeneity was obtained by electroelution of P64 from SDS-polyacrylamide gels. Monospecific rabbit antiserum (R alpha P64) was prepared using the purified protein preparation. P64 had a native molecular mass of greater than 670 kDa and was recognized by R alpha P64 as well as by human antisera. Western blotting of leptospiral serovars and 18 other bacterial species with R alpha P64 showed that P64 was cross-reactive with an equivalent antigen in a wide range of bacteria, indicating that it belongs to a family of antigens previously designated 'common antigen'. This putative common antigen from Leptospira appears to have a sub-surface location, but its function is not yet known. 相似文献
23.
The Scientific Board of the California Medical Association presents the following inventory of items of progress in preventive medicine and public health. Each item, in the judgment of a panel of knowledgeable physicians, has recently become reasonably firmly established, both as to scientific fact and important clinical significance. The items are presented in simple epitome, and an authoritative reference, both to the item itself and to the subject as a whole, is generally given for those who may be unfamiliar with a particular item. The purpose is to assist busy practitioners, students, researchers, or scholars to stay abreast of these items of progress in preventive medicine and public health that have recently achieved a substantial degree of authoritative acceptance, whether in their own field of special interest or another.The items of progress listed below were selected by the Advisory Panel to the Section on Preventive Medicine and Public Health of the California Medical Association, and the summaries were prepared under its direction. 相似文献
24.
Thomas AA Le Huerou Y De Meese J Gunawardana I Kaplan T Romoff TT Gonzales SS Condroski K Boyd SA Ballard J Bernat B DeWolf W Han M Lee P Lemieux C Pedersen R Pheneger J Poch G Smith D Sullivan F Weiler S Wright SK Lin J Brandhuber B Vigers G 《Bioorganic & medicinal chemistry letters》2008,18(6):2206-2210
Tumor cells extensively utilize the pentose phosphate pathway for the synthesis of ribose. Transketolase is a key enzyme in this pathway and has been suggested as a target for inhibition in the treatment of cancer. In a pharmacodynamic study, nude mice with xenografted HCT-116 tumors were dosed with 1 ('N3'-pyridyl thiamine'; 3-(6-methyl-2-amino-pyridin-3-ylmethyl)-5-(2-hydroxy-ethyl)-4-methyl-thiazol-3-ium chloride hydrochloride), an analog of thiamine, the co-factor of transketolase. Transketolase activity was almost completely suppressed in blood, spleen, and tumor cells, but there was little effect on the activity of the other thiamine-utilizing enzymes alpha-ketoglutarate dehydrogenase or glucose-6-phosphate dehydrogenase. Synthesis and SAR of transketolase inhibitors is described. 相似文献
25.
Diniz YS Cicogna AC Padovani CR Silva MD Faine LA Galhardi CM Rodrigues HG Novelli EL 《Canadian journal of physiology and pharmacology》2003,81(11):1042-1048
Dietary modification ought to be the first line of strategy in prevention of the development of cardiac disease. The purpose of this study was to investigate whether dietary restriction, dietary-fibre-enriched diet, and their interactions might affect antioxidant capacity and oxidative stress in cardiac tissue. Male Wistar rats (180-200 g; n=10) were divided into four groups: control ad libitum diet (C), 50% restricted diet (DR), fed with fibre-enriched diet (F), and 50% restricted fibre-enriched diet (DR-F). After 35 days of the treatments, F, DR, and DR-F rats showed low cholesterol, LDL-cholesterol, and triacylglycerol, and high HDL-cholesterol in serum. The DR, DR-F, and F groups had decreased myocardial lipoperoxide and lipid hydroperoxide. The DR-F and F treatments increased superoxide dismutase and glutatione peroxidase (GSH-Px). The DR treatment increased GSH-Px and catalase activities. Dietary fibre beneficial effects were related to metabolic alterations. The F and DR-F groups showed high cardiac glycogen and low lactate dehydrogenase/citrate synthase ratios, indicating diminished anaerobic and elevated aerobic myocardial metabolism in these animals. There was no synergistic effect between dietary restriction and dietary fibre addition, since no differences were observed in markers of oxidative stress in the F and DR-F groups. Dietary fibre supplementation, rather than energy intake and dietary restriction, appears to be the main process retarding oxidative stress in cardiac tissue. 相似文献
26.
Land use intensification alters ecosystem multifunctionality via loss of biodiversity and changes to functional composition 总被引:5,自引:0,他引:5
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Eric Allan Pete Manning Fabian Alt Julia Binkenstein Stefan Blaser Nico Blüthgen Stefan Böhm Fabrice Grassein Norbert Hölzel Valentin H. Klaus Till Kleinebecker E. Kathryn Morris Yvonne Oelmann Daniel Prati Swen C. Renner Matthias C. Rillig Martin Schaefer Michael Schloter Barbara Schmitt Ingo Schöning Marion Schrumpf Emily Solly Elisabeth Sorkau Juliane Steckel Ingolf Steffen‐Dewenter Barbara Stempfhuber Marco Tschapka Christiane N. Weiner Wolfgang W. Weisser Michael Werner Catrin Westphal Wolfgang Wilcke Markus Fischer 《Ecology letters》2015,18(8):834-843
Global change, especially land‐use intensification, affects human well‐being by impacting the delivery of multiple ecosystem services (multifunctionality). However, whether biodiversity loss is a major component of global change effects on multifunctionality in real‐world ecosystems, as in experimental ones, remains unclear. Therefore, we assessed biodiversity, functional composition and 14 ecosystem services on 150 agricultural grasslands differing in land‐use intensity. We also introduce five multifunctionality measures in which ecosystem services were weighted according to realistic land‐use objectives. We found that indirect land‐use effects, i.e. those mediated by biodiversity loss and by changes to functional composition, were as strong as direct effects on average. Their strength varied with land‐use objectives and regional context. Biodiversity loss explained indirect effects in a region of intermediate productivity and was most damaging when land‐use objectives favoured supporting and cultural services. In contrast, functional composition shifts, towards fast‐growing plant species, strongly increased provisioning services in more inherently unproductive grasslands. 相似文献
27.
Caterina Penone Eric Allan Santiago Soliveres María R Felipe‐Lucia Martin M Gossner Sebastian Seibold Nadja K. Simons Peter Schall Fons van der Plas Peter Manning Rubn D. Manzanedo Steffen Boch Daniel Prati Christian Ammer Jürgen Bauhus Franois Buscot Martin Ehbrecht Kezia Goldmann Kirsten Jung Jrg Müller Jrg C. Müller Rodica Pena Andrea Polle Swen C. Renner Liliane Ruess Ingo Schnig Marion Schrumpf Emily F. Solly Marco Tschapka Wolfgang W. Weisser Tesfaye Wubet Markus Fischer 《Ecology letters》2019,22(1):170-180
While forest management strongly influences biodiversity, it remains unclear how the structural and compositional changes caused by management affect different community dimensions (e.g. richness, specialisation, abundance or completeness) and how this differs between taxa. We assessed the effects of nine forest features (representing stand structure, heterogeneity and tree composition) on thirteen above‐ and belowground trophic groups of plants, animals, fungi and bacteria in 150 temperate forest plots differing in their management type. Canopy cover decreased light resources, which increased community specialisation but reduced overall diversity and abundance. Features increasing resource types and diversifying microhabitats (admixing of oaks and conifers) were important and mostly affected richness. Belowground groups responded differently to those aboveground and had weaker responses to most forest features. Our results show that we need to consider forest features rather than broad management types and highlight the importance of considering several groups and community dimensions to better inform conservation. 相似文献
28.
Solly F Angelot F Garand R Ferrand C Seillès E Schillinger F Decobecq A Billot M Larosa F Plouvier E Deconinck E Legrand F Saas P Rohrlich PS Garnache-Ottou F 《Cytometry. Part A》2012,81(1):17-24
Minimal residual disease (MRD) has emerged as a major prognostic factor for monitoring patients with B-lineage acute lymphoblastic leukemia (B-ALL). The quantification of MRD by flow cytometry (FC) is based on the identification of a leukemia-associated phenotype (LAP). Because phenotypic switch is common during treatment, multiple LAPs must be available and used for MRD detection over time. We evaluated the potential usefulness of CD304 as a new marker for monitoring MRD. CD304 was expressed in 48% of B-ALL (24/50) with discriminative fluorescence intensity compared with CD304-negative normal B-cell precursors (n = 15). The sensitivity of CD304-based MRD detection reached 10(-4), as with some of established LAPs. The stability of CD304 expression evaluated during therapy and at relapse confirms the usefulness of this marker for MRD quantification. Finally, CD304 was repeatedly expressed in patients with TEL-AML1 gene rearrangement, which warrants further investigation on its potential relevance as a prognosis marker or therapeutic target. 相似文献
29.
Thomas AA De Meese J Le Huerou Y Boyd SA Romoff TT Gonzales SS Gunawardana I Kaplan T Sullivan F Condroski K Lyssikatos JP Aicher TD Ballard J Bernat B DeWolf W Han M Lemieux C Smith D Weiler S Wright SK Vigers G Brandhuber B 《Bioorganic & medicinal chemistry letters》2008,18(2):509-512
Inhibition of the thiamine-utilizing enzyme transketolase (TK) has been linked with diminished tumor cell proliferation. Most thiamine antagonists have a permanent positive charge on the B-ring, and it has been suggested that this charge is required for diphosphorylation by thiamine pyrophosphokinase (TPPK) and binding to TK. We sought to make neutral thiazolium replacements that would be substrates for TPPK, while not necessarily needing thiamine transporters (ThTr1 and ThTr2) for cell penetration. The synthesis, SAR, and structure-based rationale for highly potent non-thiazolium TK antagonists are presented. 相似文献
30.
Faine LA Diniz YS Galhardi CM Rodrigues HG Burneiko RC Santana LS Cicogna AC Novelli EL 《Canadian journal of physiology and pharmacology》2004,82(11):969-975
Caloric intake is higher than recommended in many populations. Therefore, enhancing olive oil intake alone may not be the most effective way to prevent cardiovascular diseases. The purpose of the present study was to analyse the association of olive oil and dietary restriction on lipid profile and myocardial antioxidant defences. Male Wistar rats (180-200 g, n = 6) were divided into 4 groups: control ad libitum diet (C), 50% restricted diet (DR), fed ad libitum and supplemented with olive oil (3 mL/(kg x day)) (OO), and 50% restricted diet and supplemented with olive oil (DROO). After 30 days of treatments, OO, DR, and DROO groups had increased total cholesterol and high-density lipoprotein cholesterol concentrations. DR and DROO animals showed decreased low-density lipoprotein cholesterol. DROO had the lowest low-density lipoprotein cholesterol concentration. Total lipids and triacylglycerols were raised by dietary restriction and diminished by olive oil. OO rats had higher myocardial superoxide dismutase and lower catalase and glutathione peroxidase activities than C rats. DR and DROO showed enhanced cardiac superoxide dismutase, catalase, and glutathione peroxidase activities from the control. Olive oil supplementation alone improved the lipid profile but was more effective when coupled with dietary restriction. There was a synergistic beneficial action of dietary restriction and olive oil on serum lipids and myocardial antioxidant defences. 相似文献