通过体内基因转染改变Meis1在围着床期小鼠子宫中的表达影响胚胎着床

通过宫腔内脂质体转染改变小鼠子宫内Meis1基因的表达水平,研究其对子宫内膜容 受性的影响,从而推测Meis1基因在胚胎着床中的作用.选择8～12周龄昆明小鼠,于妊娠 第2 ｄ,通过向小鼠宫腔内注入Meis1基因表达质粒和siRNA表达质粒及其各自的对照质粒 ,在妊娠第5ｄ, 提取小鼠子宫mRNA 和蛋白质行半定量RT-PCR 和免疫组化分析,观察各组小鼠子宫内膜Meis1和整合素β3的表达变化.在妊娠第9 ｄ,观察Meis1基因上调组及其对 照组、Meis1基因下调及其对照组妊娠率和胚胎着床数的差异.结果显示,Meis1基因下调组胚胎着床率明显低于对照组（P<0.05）,Meis1基因上调组胚胎着床率略高于对照组,但无统计学意义（P>0.05）;Meis1基因上调组其整合素β3的表达高于其对照组,Meis1基因下调组 整合素β3的表达低于其对照组,差异均有显著性（P<0.05）.以上观察结果表明,Meis1基因表达下降可明显减少胚胎着床率,影响整合素β3的表达.Meis1基因表达提高则可促进整合素β3的表达. 因此,Meis1可能作为1种子宫内膜容受分子,在胚胎着床中发挥重要作用.     

利用SSR标记鉴定西瓜杂交种纯度的研究

以2个西瓜杂交品种(系)的种子黑公子和04-17及其亲本为材料,用SSR标记技术研究杂种与其双亲之间的扩增谱带多态性,以甄别真假杂种.结果发现,所试验的52对SSR引物中有13对引物分别在2个西瓜杂交种和其双亲之间存在扩增条带的多态性,表现为:多数SSR引物对自交系的扩增只出现1条带,但部分引物在某些自交系中扩增出2条带,杂交种条带均为父母本的互补型,很适合做杂交种纯度鉴定.用引物CMCT134b对黑公子和引物CMGA165对04-17进行了各100粒单种子SSR鉴定,所测纯度分别为96%和100%,与田间纯度95.6%和99.7%非常接近,表明SSR标记技术在西瓜杂交种子纯度室内快速检测中的应用前景.     

里白的组织培养与快速繁殖

1植物名称 里白[Hicriopteris glauca（Thunb.）Ching]。 2材料类别 成熟孢子。 3培养条件孢子萌发培养基：（1）1/2MS;配子体（原叶体）增殖培养基：（2）MS＋6-BA0.5mg·L-1（单位不同）     

D-半乳糖致衰老模型大鼠的学习记忆和行为学观察及中药干预作用

目的观察D-半乳糖致衰老模型大鼠学习记忆能力和行为学情况,并探讨中药的干预作用。方法大鼠每日一次颈背部皮下注射5%D-半乳糖100 mg/kg。诱导大鼠衰老模型,连续7周,观察衰老模型大鼠的自主活动次数、空间记忆能力、主动回避遭受电击能力、探究活动等行为学表现和学习记忆能力,并用抗衰老片与首乌延寿片进行干预,观察中药的干预作用。结果皮下注射D-半乳糖造模后,衰老模型大鼠自主活动次数显著减少（P〈0.05,P〈0.01）,水迷宫试验探索路径长度和搜台潜伏期显著延长（P〈0.05,P〈0.01）,旷场试验移动路程长度和直立次数显著减少（P〈0.01）,穿梭回避试验平均潜伏期、进入错误区时间显著增加（P〈0.05,P〈0.01）。给予抗衰老片与首乌延寿片干预后,衰老大鼠的自主活动次数显著增加（P〈0.01）,水迷宫试验探索路径长度和搜台潜伏期显著缩短（P〈0.01）,旷场试验移动路程长度和直立次数显著增加（P〈0.01）,穿梭回避试验平均潜伏期、进入错误区时间显著减少（P〈0.05,P〈0.01）。结论D-半乳糖致衰老模型大鼠的自主活动次数减少,对新环境探索能力下降,学习记忆力下降;抗衰老片与首乌延寿片等中药可有效增强衰老模型大鼠的行为活动,提高衰老模型大鼠的学习记忆能力。     

不同地理来源头花蓼的遗传多样性与没食子酸含量相关性分析

应用ISSR-PCR和HPLC方法研究了头花蓼的遗传多样性与没食子酸之间的关系。ISSR结果显示居群总的遗传变异较大(Ht=0.2746),居群内的遗传变异较小(Hs=0.0804),居群间的遗传分化大于居群内的遗传分化。没食子酸含量经SPSS17.0分析显示,各居群间和居群内个体的没食子酸含量差异较大,居群间没食子酸含量范围在0.1738%～0.3306%,其中云南腾冲县,贵州台江县、纳雍县、余庆县、晴隆和毕节县居群间没食子酸含量差异均达到显著水平。通过对头花蓼48个地理居群的遗传多样性与没食子酸含量的相关分析,表明云南腾冲,贵州台江、毕节市亮岩镇、晴隆、盘县居群的遗传多样性指数、没食子酸含量较高,不仅可以作为人工育种的选育材料,而且对于头花蓼种植适生地区划研究具有很好的参考价值。     

颈交感神经阻滞对严重烧伤大鼠肾脏的保护作用及其机制研究

目的:观察颈交感神经阻滞(CSB)对严重烧伤大鼠肾脏的保护作用,并对其可能机制进行初步探讨。方法:制作放烧严重烧伤伤大鼠大鼠模型,在伤后即刻及连续4天内进行双侧颈交感神经阻滞,观察生命体征、肾功能指标、肾脏组织学改变、血流灌注、抗氧化能力、肾组织Bcl-2表达改变。结果:经过颈交感神经阻滞处理后,伤后各时相点动物肾功能均较对照组为好,组织学病变减轻,器官血流量下降幅度减小,总抗氧化能力好于对照组,免疫组化显示CSB组肾组织中Bcl-2的表达较严重烧伤组明显增高。结论:颈交感神经阻滞可改善严重烧伤大鼠肾脏血液灌注,增强抗氧化能力,预防伤后肾脏功能损害。     

URA3基因影响青蒿酸酿酒酵母工程菌中试发酵产量

CRISPR/Cas9基因编辑技术已经被广泛应用于工程酿酒酵母的基因插入、基因替换和基因敲除,通过使用选择标记进行基因编辑具有简单高效的特点。前期利用CRISPR/Cas9系统敲除青蒿酸生产菌株酿酒酵母(Saccharomyces cerevisiae) 1211半乳糖代谢负调控基因GAL80,获得菌株S. cerevisiae 1211-2,在不添加半乳糖诱导的情况下,青蒿酸摇瓶发酵产量达到了740 mg/L。但在50 L中试发酵实验中,S. cerevisiae 1211-2很难利用对青蒿酸积累起到决定性作用的碳源-乙醇,青蒿酸的产量仅为亲本菌株S.cerevisiae 1211的20%–25%。我们推测因遗传操作所需的筛选标记URA3突变,影响了其生长及青蒿酸产量。随后我们使用重组质粒pML104-KanMx4-u连同90 bp供体DNA成功恢复了URA3基因,获得了工程菌株S. cerevisiae 1211-3。S. cerevisiae 1211-3能够在葡萄糖和乙醇分批补料的发酵罐中正常生长,其青蒿酸产量超过20g/L,与亲本菌株产量相当。研究不但获得了不加半乳糖诱导的青...     

严重急性呼吸综合征、中东呼吸综合征和2019冠状病毒病治疗研究进展

严重急性呼吸综合征2019(sever acute respiratory syndrome,SARS)、中东呼吸综合征(Middle East respiratory syndrome,MERS)和2019冠状病毒病(corona virus disease 2019,COVID-19)对全世界人民造成了严重的经济损...     

Apodeme and ovarian development as predictors of physiological status in Bactrocera cacuminata (Hering) (Diptera: Tephritidae)

Abstract Assessing resource-use by different life stages of insects is crucial to our understanding of their ecology. Development of appropriate methods by which different physiological states can be identified is therefore significant. We develop one such method for the dacine fly Bactrocera cacuminata (Hering) (Diptera: Tephritidae). In male flies, the ejaculatory apodeme, a structural part of the reproductive endoskeleton, grows as a function of age of the fly. This growth pattern is sigmoid, reaching a maximum threshold that corresponds with the attainment of sexual maturity, as measured by the incidence of first mating. Similarly, in female flies ovarian development appears to be a reliable indicator of sexual maturity. Assuming that similar growth thresholds occur in wild flies, it is possible to use apodeme and ovarian development as measures of physiological/reproductive status in this species.     

Positive selection in MAOA gene is human exclusive: determination of the putative amino acid change selected in the human lineage

Monoamine oxidase A (MAOA) is the X-linked gene responsible for deamination and subsequent degradation of several neurotransmitters and other amines. Among other activities, the gene has been shown to play a role in locomotion, circadian rhythm, and pain sensitivity and to have a critical influence on behavior and cognition. Previous studies have reported a non-neutral evolution of the gene attributable to positive selection in the human lineage. To determine whether this selection was human-exclusive or shared with other species, we performed a population genetic analysis of the pattern of nucleotide variation in non-human species, including bonobo, chimpanzee, gorilla, and orangutan. Footprints of positive selection were absent in all analyzed species, suggesting that positive selection has been recent and unique to humans. To determine which human-unique genetic changes could have been responsible for this differential evolution, the coding region of the gene was compared between human, chimpanzee, and gorilla. Only one human exclusive non-conservative change is present in the gene: Glu151Lys. This human substitution affects protein dimerization according to a three-dimensional structural model that predicts a non-negligible functional shift. This is the only candidate position at present to have been selected to fixation in humans during an episode of positive selection. Divergence analysis among species has shown that, even under positive selection in the human lineage, the MAOA gene did not experience accelerated evolution in any of the analyzed lineages, and that tools such as K_a/K_s would not have detected the selective history of the gene.