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21.
Several studies have shown that neuronal cell death due to apoptosis is the major reason for cognitive decline in Alzheimer's disease. In this study, we report the anti-apoptotic effects of three Salvia species from Iran-S. choloroleuca, S. mirzayanii and S. santolinifolia-against H(2)O(2)-induced cytotoxicity in neuron-like PC12 cells. We showed that these antioxidant species could interfere with the intrinsic pathway of apoptosis by attenuating Bax/Bcl-2 ratio, decreasing outer mitochondrial membrane break and decreasing cytochrome c release to cytoplasm. Interestingly, we found that these species were able to replenish reduced glutathione level which affects cellular redox status and cytochrome c activity. Moreover, the decreased level of caspase-3, the executioner caspase, resulted in decrease of PARP-1 cleavage. Anti-apoptotic effects of these species along with their antioxidant effects, may represent a promising approach for treatment of neurodegenerative diseases. 相似文献
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S. Madapa Z. Tusi D. Sridhar A. Kumar M.I. Siddiqi K. Srivastava A. Rizvi R. Tripathi S.K. Puri G.B. Shiva Keshava P.K. Shukla S. Batra 《Bioorganic & medicinal chemistry》2009,17(1):203-221
A total of 80 new 2-methyl-6-ureido-4-quinolinamides were synthesized and evaluated for their antimalarial activity. Several analogs elicited the antimalarial effect at MIC of 0.25 mg/mL against the chlooquine-sensitive P. falciparum strain. The IC50 values of the active compounds were observed to be in ng/mL range and two of the analogs have better IC50 value than the standard chloroquine. In the in vivo assay against mdr CQ resistant P. yoelii N67/P. yoelii nigeriensis, however, none of the compound showed complete suppression of parasitemia on day 7. One of the compounds displayed significant antibacterial effect against several strains of bacteria and was many-fold better than the standard drug gentamicin. 相似文献
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M. T. Tusié-Luna Salvador Ramírez-Jiménez Ma. Luisa Ordóñez-Sánchez Javier Cabello-Villegas Nelly Altamirano-Bustamante Raúl Calzada-León Carlos Robles-Valdés Fernando Mendoza-Morfín Juan Pablo Méndez Margarita Terán-García 《Human genetics》1996,98(3):376-379
Steroid 21-hydroxylase deficiency is caused by mutations in the CYP21 gene. Approximately 95% of mutant alleles are generated
by recombination events between the acitve gene CYP21 and its highly homologous pseudogene, CYP21P. Deletion alleles are generated
by unequal crossing over, while point mutations are the result of gene conversion events. Deletions account for 20–25% of
the 21-hydroxylase deficiency alleles in most populations studied. We have looked for deletions among 53 unrelated Mexican
patients with steroid 21-hydroxylase deficiency and found that deletions represent less than 1% of the disease alleles. These
findings suggest that nearly all mutant alleles in our patient population contain point mutations and that the low representation
of deletion alleles among clinically diagnosed patients may be due to missing detection of salt wasters, mainly males, who
may die during the neonatal period.
Received: 17 November 1995 / Revised: 29 February 1996, 12 April 1996 相似文献
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Parisa Shabani H Naeimi Khaledi Maani Beigy Solaleh Emamgholipour Eskandar Parvaz Hossein Poustchi Mahmood Doosti 《PloS one》2015,10(3)
Nonalcoholic fatty liver disease (NAFLD) is considered as one of the most common liver diseases. It is robustly linked to obesity and insulin resistance and is regarded as hepatic manifestation of metabolic syndrome (MetS). Adipokines are involved in the pathophysiology of liver diseases. The aim of this study was to evaluate the plasma concentrations of CTRP1 (complement-C1q TNF-related protein 1) in 22 patients with NAFLD, 22 patients with type 2 diabetes mellitus (T2DM), 22 patients with NAFLD+T2DM and 21 healthy controls, as well as their correlation with the level of metabolic and hepatic parameters. Plasma concentration of CTRP1 was measured with ELISA method. Plasma concentration of CTRP1 in patients with NAFLD, T2DM and NAFLD+T2DM were significantly higher than healthy subjects (p<0.0001). Moreover, we observed significant positive correlations between plasma level of CTRP1 and fasting blood glucose (FBG) (p<0.001), homeostasis model assessment of insulin resistance (HOMA-IR) (p<0.001), body mass index (BMI) (p = 0.001), alanine amino transferase (ALT) (p = 0.002), gamma glutamyl transferase (γ-GT) (p<0.001) and liver stiffness (LS) (p<0.001). Our results indicate the strong association of CTRP1 with insulin resistance in NAFLD. Also, it seems that CTRP1 can be considered as an emerging biomarker for NAFLD, however, more studies are necessary to unravel the role of CTRP1 in NAFLD pathogenesis. 相似文献
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Jannat Ali Pour Naghmeh Meshkani Reza Toolabi Karamollah Mohassel Azadi Samaneh Zand Shahabedin Emamgholipour Solaleh 《Molecular biology reports》2020,47(5):3459-3468
Molecular Biology Reports - It is well-established that an impaired adipose tissue function and morphology caused by a dysregulated gene expression contribute substantially to obesity. Nowadays,... 相似文献
28.
Sahebekhtiari N Nochi Z Eslampour MA Dabiri H Bolfion M Taherikalani M Khoramian B Zali MR Emaneini M 《Acta microbiologica et immunologica Hungarica》2011,58(2):113-121
Staphylococcus aureus is considered one of the most important food borne pathogens. A total of 111 isolates of S. aureus were cultured from raw milk samples during January 2009 to June 2009 from Tehran and Mashhad. The coagulase gene polymorphism and the prevalence of classical enterotoxin genes of S. aureus strains were determined by PCR-RFLP (restriction fragment length polymorphism) and Multiplex-PCR. Disk diffusion method was used to determine the susceptibility of isolates to antimicrobial agents as instructed by Clinical and Laboratory Standards Institute. Sixty-seven % of the isolates harboured one or more enterotoxin genes. The most prevalent gene was sec, found in 59 % of the isolates. Approximately 8% of the isolates were positive for sea, seb and sed genes. Only one isolate had see gene. The rate of coexistence of enterotoxin genes was 14%. All S. aureus isolates were susceptible to ciprofloxacin, gentamicin, imipenem, minocycline, oxacillin and vancomycin. They were resistant to ampicillin (64%), penicillin (56%), clindamycin (22%), tetracycline (22%), doxycycline (19%), teicoplanin (13%), rifampin (2%) and trimethoprim-sulfamethoxazole (2%). On the basis of coagulase gene analysis of 111 S. aureus isolates, the PCR products of 56 isolates were digested with Alu I that produced three distinct patterns. These data indicate the high prevalence of enterotoxigenic S. aureus in raw bovine milk in Tehran and Mashhad, and highlight the importance of proper quality control of dairy products for public health. 相似文献
29.
Nuclear factor erythroid 2-related factor 2 (Nrf2) coordinates the up-regulation of cytoprotective genes via the antioxidant
response element (ARE). There is significant evidence that oxidative stress is a critical event in the pathogenesis of AD.
Considering the protective role of Nrf2 against oxidative injury, we studied to determine whether in vivo toxicity of amyloid
β (Aβ) can be attenuated by tBHQ, an Nrf2 stabilizer, Using an Aβ injection model. We demonstrated that pre-activation of
endogenous Nrf2 by tBHQ attenuated Aβ-induced caspase-3 expression. tBHQ enhanced GSH, decreased MDA level, and inhibited
NF-κB. This investigation provides the first documentation of tBHQ’s neuroprotective effect through decrease of Aβ accumulation
in rat brain. Our results show the involvement of Hsp-70 in this protective effect. In summary tBHQ treatment for 1 week prior
to Aβ injection protected against the oxidative damage, apoptosis and Aβ accumulation in rats. 相似文献
30.
Canizales-Quinteros S Aguilar-Salinas CA Ortiz-López MG Rodríguez-Cruz M Villarreal-Molina MT Coral-Vázquez R Huertas-Vázquez A Hernández-Caballero A López-Alarcón M Brito-Zurita OR Domínguez-Banda A Martinez-Sánchez LR Canto-de Cetina T Vilchis-Dorantes G Rosas-Vargas H Granados-Silvestre MA Medeiros-Domingo A Menjivar M Tusié-Luna MT 《Human biology; an international record of research》2007,79(1):111-119
Previous studies have sought to associate the Pro12Ala variant of the peroxisome proliferator-activated receptor gamma2 (PPARG2) gene with type 2 diabetes, insulin resistance, and obesity, with controversial results. We have determined the Pro12Ala variant frequency in 370 nondiabetic Mexican Mestizo subjects and in five Mexican Amerindian groups and have investigated its possible association with lipid metabolism, insulin serum levels, and obesity in three of these populations. Two independent case-control studies were conducted in 239 nondiabetic individuals: 135 case subjects (BMI > or = 25 kg/m2) and 104 control subjects (BMI < 25 kg/m2). The PPARG2 Ala12 allele frequency was higher in most Amerindian populations (0.17 in Yaquis, 0.16 in Mazahuas, 0.16 in Mayans, and 0.20 in Triquis) than in Asians, African Americans, and Caucasians. The Pro12Ala and Ala12Ala (X12Ala) genotypes were significantly associated with greater BMI in Mexican Mestizos and in two Amerindian groups. X12Ala individuals had a higher risk of overweight or obesity than noncarriers in Mestizos (OR = 3.67; 95% CI, 1.42-9.48; p = 0.007) and in Yaquis plus Mazahuas (OR = 3.21; 95% CI, 1.27-8.11; p = 0.013). Our results provide further support of the association between the PPARG2 Ala12 allele and risk of overweight or obesity in Mestizos and two Amerindian populations from Mexico. 相似文献