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51.
Denis Susorov Tatiana Mikhailova Alexander Ivanov Elizaveta Sokolova Elena Alkalaeva 《Nucleic acids research》2015,43(6):3332-3343
Stabilization of the ribosomal complexes plays an important role in translational control. Mechanisms of ribosome stabilization have been studied in detail for initiation and elongation of eukaryotic translation, but almost nothing is known about stabilization of eukaryotic termination ribosomal complexes. Here, we present one of the mechanisms of fine-tuning of the translation termination process in eukaryotes. We show that certain deacylated tRNAs, remaining in the E site of the ribosome at the end of the elongation cycle, increase the stability of the termination and posttermination complexes. Moreover, only the part of eRF1 recognizing the stop codon is stabilized in the A site of the ribosome, and the stabilization is not dependent on the hydrolysis of peptidyl-tRNA. The determinants, defining this property of the tRNA, reside in the acceptor stem. It was demonstrated by site-directed mutagenesis of tRNAVal and construction of a mini-helix structure identical to the acceptor stem of tRNA. The mechanism of this stabilization is different from the fixation of the unrotated state of the ribosome by CCA end of tRNA or by cycloheximide in the E site. Our data allow to reveal the possible functions of the isodecoder tRNAs in eukaryotes. 相似文献
52.
Nina S. Brykova Dmitriy A. Gusarov Irene V. Sokolova Valentine D. Gusarova Tatjana V. Vorobjeva Dmitry I. Bairamashvili 《Process Biochemistry》2011,46(10):2036-2043
It was shown that H1 type histones possess anticancer activity and could be utilized in therapy of acute myeloid leukemia. We developed an experimental pilot-scale technology of the recombinant histone H1.3 variant production. The downstream process includes acidic extraction of the target protein from the culture broth, ion-exchange, reversed-phase and size-exclusion chromatography, and freeze-drying. The accent was made on the reduction of bacterial endotoxin contamination of the active pharmaceutical ingredient. Highly efficient downstream strategy of the target protein depyrogenation is discussed in the paper. The developed technology allows the production of the protein with high yield (approx. 110 g per 1000 L of the culture broth) and of high purity (estimated productivity is 75–100 g/month). The activity and purity of the active pharmaceutical ingredients produced for clinical trials were confirmed by different tests including ultra performance liquid chromatography, size exclusion chromatography, ELISA, SDS–PAGE, gel-clot LAL-test. Clinical trials were started in the Russian Federation. 相似文献
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54.
Diuzhikova NA Sokolova NE Vaĭdo AI Shiriaeva NV Lopatina NG Levkovich IuI 《Zhurnal vysshe? nervno? deiatelnosti imeni I P Pavlova》2001,51(4):511-513
Quantitative characteristics (the area and number of chromocenters) of the interphase C-heterochromatin in the nuclei of pyramidal neurons of the midbrain reticular formation, sensorimotor cortex, and hippocampus (CA3) of rat strains with different genetically determined excitability were studied in the normal state of the animals and after exposure to a short-term emotional pain stress. The results indicate a relationship between the excitability of the nervous system and structural-functional state of the neuronal interphase heterochromatin. The role of cytogenetic features of different brain structures in the CNS functioning and behavior and their relation with genetically determined excitability of the nervous system are discussed. 相似文献
55.
D V Sviatoslavov N V Dmitrieva I N Petukhova E V Kulaga I I Shil'nikova S A D'iakova T A Kalinchuk E N Sokolova 《Antibiotiki i khimioterapii͡a》2006,51(11-12):37-45
The most important problem of onkology, i. e. antibiotic prophylaxis and treatment of postoperative infectious complications is touched upon in the paper. The current publications on the problem are discussed and the autors' experience on the treatment is described. The spectrum of the main pathogens of wound infections in patients with head and neck tumors is considered. 相似文献
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58.
Semenova MV Sinitsyna OA Morozova VV Fedorova EA Gusakov AV Okunev ON Sokolova LM Koshelev AV Bubnova IuP Sinitsyn AP 《Prikladnaia biokhimiia i mikrobiologiia》2006,42(6):681-685
A new enzyme preparation of fungal pectin lyase (EC 4.2.2.10) was shown to be useful for the production of cranberry juice and clarification of apple juice in the food industry. A comparative study showed that the preparation of pectin lyase is competitive with commercial pectinase products. The molecular weight of homogeneous pectin lyase was 38 kDa. Properties of the homogeneous enzyme were studied. This enzyme was most efficient in removing highly esterified pectin. 相似文献
59.
The effects of 12 elements on the formation and accumulation of isoquinolines were studied in opium poppy seedlings (Papaver somniferum L.). Three types of concentration dependences of the effects of the elements under study were determined. The elements served as activators (Co, Mo, W, Cr, and Cu) or inhibitors of these processes (B, Fe, V, Mn, and Ca). The molecular mechanisms of action of these regulators are discussed. The optimum concentrations of Co and Mo were tested under combined treatment conditions with these elements. 相似文献
60.
Sokolova ES Liudyno VI Simbirtsev AS Klimenko VM 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2006,92(3):299-307
Behavioral effects of low, sub-pyrogenic doses of IL-lbeta were investigated in rats. It was shown that increasing of anxiety level started to occur as of the 5th day of the administration course. The data obtained suggest importance of pro-inflammatory cytokines in pathogenesis of psycho-emotional disorders. 相似文献