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71.
Both the RGS Domain and the Six C-Terminal Amino Acids of Mouse Axin Are Required for Normal Embryogenesis 下载免费PDF全文
Ian V. Chia Min Jung Kim Keiji Itoh Sergei Y. Sokol Frank Costantini 《Genetics》2009,181(4):1359-1368
Axin is a negative regulator of canonical Wnt signaling, which promotes the degradation of β-catenin, the major effector in this signaling cascade. While many protein-binding domains of Axin have been identified, their significance has not been evaluated in vivo. Here, we report the generation and analysis of mice carrying modified Axin alleles in which either the RGS domain or the six C-terminal amino acids (C6 motif) were deleted. The RGS domain is required for APC-binding, while the C6 motif has been implicated in the activation of c-Jun N-terminal kinase, but is not required for the effects of Axin on the Wnt/β-catenin pathway, in vitro. Both mutant Axin alleles caused recessive embryonic lethality at E9.5–E10.5, with defects indistinguishable from those caused by a null allele. As Axin-ΔRGS protein was produced at normal levels, its inability to support embryogenesis confirms the importance of interactions between Axin and APC. In contrast, Axin-ΔC6 protein was expressed at only 25–30% of the normal level, which may account for the recessive lethality of this allele. Furthermore, many AxinΔC6/ΔC6 embryos that were heterozygous for a β-catenin null mutation survived to term, demonstrating that early lethality was due to failure to negatively regulate β-catenin. 相似文献
72.
Functional and structural diversity of the human Dickkopf gene family 总被引:28,自引:0,他引:28
Krupnik VE Sharp JD Jiang C Robison K Chickering TW Amaravadi L Brown DE Guyot D Mays G Leiby K Chang B Duong T Goodearl AD Gearing DP Sokol SY McCarthy SA 《Gene》1999,238(2):301-313
Wnt proteins influence many aspects of embryonic development, and their activity is regulated by several secreted antagonists, including the Xenopus Dickkopf-1 (xDkk-1) protein. xDkk-1 inhibits Wnt activities in Xenopus embryos and may play a role in induction of head structures. Here, we characterize a family of human Dkk-related genes composed of Dkk-1, Dkk-2, Dkk-3, and Dkk-4, together with a unique Dkk-3 related protein termed Soggy (Sgy). hDkks 1-4 contain two distinct cysteine-rich domains in which the positions of 10 cysteine residues are highly conserved between family members. Sgy is a novel secreted protein related to Dkk-3 but which lacks the cysteine-rich domains. Members of the Dkk-related family display unique patterns of mRNA expression in human and mouse tissues, and are secreted when expressed in 293T cells. Furthermore, secreted hDkk-2 and hDkk-4 undergo proteolytic processing which results in cleavage of the second cysteine-rich domain from the full-length protein. Members of the human Dkk-related family differ not only in their structures and expression patterns, but also in their abilities to inhibit Wnt signaling. hDkk-1 and hDkk-4, but not hDkk-2, hDkk-3 or Sgy, suppress Wnt-induced secondary axis induction in Xenopus embryos. hDkk-1 and hDkk-4 do not block axis induction triggered either by Xenopus Dishevelled (Xdsh) or Xenopus Frizzled-8 (Xfz8), both of which function to transduce signals from Wnt ligands. Thus, hDkks 1 and 4 may inhibit Wnt activity by a mechanism upstream of Frizzled. Our findings highlight the structural and functional heterogeneity of human Dkk-related proteins. 相似文献
73.
Renata Grzywa Anna M. Sokol Marcin Sieńczyk Magdalena Radziszewicz Beata Kościołek Michael P. Carty Józef Oleksyszyn 《Bioorganic & medicinal chemistry》2010,18(8):2930-2936
A series of new aromatic monoesters of α-aminoaralkylphosphonic acids were synthesized by selective hydrolysis of corresponding aromatic diesters of α-aminoaralkylphosphonic acids. New potential inhibitors of aminopeptidase N/CD13, an enzyme important in tumour angiogenesis, were developed. Some derivatives of the homophenylalanine and norleucine related monoaryl phosphonates displayed higher inhibition potency than corresponding α-aminoaralkylphosphonic acids toward aminopeptidase N/CD13. The effect of one of the new inhibitors on the growth of human PANC-1 and HT-1080 cell lines was examined, either alone or in combination with TNF-α. 相似文献
74.
Giuseppe Nicastro Sokol V. Todi Ezgi Karaca Alexandre M. J. J. Bonvin Henry L. Paulson Annalisa Pastore 《PloS one》2010,5(8)
Ataxin-3, the disease protein in the neurodegenerative disorder Spinocerebellar Ataxia Type 3 or Machado Joseph disease, is a cysteine protease implicated in the ubiquitin proteasome pathway. It contains multiple ubiquitin binding sites through which it anchors polyubiquitin chains of different linkages that are then cleaved by the N-terminal catalytic (Josephin) domain. The properties of the ubiquitin interacting motifs (UIMs) in the C-terminus of ataxin-3 are well established. Very little is known, however, about how two recently identified ubiquitin-binding sites in the Josephin domain contribute to ubiquitin chain binding and cleavage. In the current study, we sought to define the specific contribution of the Josephin domain to the catalytic properties of ataxin-3 and assess how the topology and affinity of these binding sites modulate ataxin-3 activity. Using NMR we modeled the structure of diUb/Josephin complexes and showed that linkage preferences are imposed by the topology of the two binding sites. Enzymatic studies further helped us to determine a precise hierarchy between the sites. We establish that the structure of Josephin dictates specificity for K48-linked chains. Site 1, which is close to the active site, is indispensable for cleavage. Our studies open the way to understand better the cellular function of ataxin-3 and its link to pathology. 相似文献
75.
76.
Kauffman LD Sokol RJ Jones RH Awad JA Rewers MJ Norris JM 《Free radical biology & medicine》2003,35(6):551-557
Oxidative stress has been linked to many diseases, but little information exists on biomarkers of oxidative stress in healthy children. The purpose of this study was to describe factors that correlate with urinary F2-isoprostanes, an indicator of oxidative stress, and to establish normal concentrations of F2-isoprostanes in children at risk to develop type 1 diabetes mellitus. Creatinine-adjusted urinary F2-isoprostanes were assessed in 342 Denver children under the age of 7 years, from whom we had collected data during 769 clinic visits from August 1997 through January 2001 (mean 2.3 visits per child). Children were identified by newborn screening for HLA-markers, of varying degrees of prediction, for the development of type 1 diabetes. Plasma antioxidants and carotenoids, age at clinic visit, vitamin supplement use, exposure to environmental tobacco smoke, gender, and race were evaluated as correlates to the degree of oxidative stress, using mixed models for longitudinal data. F2-isoprostane levels were highest in infancy and decreased nonlinearly until 7 years. Female gender, HLA-DR3/4 genotype, higher plasma gamma-tocopherol:total lipids ratio, and lower alpha-carotene:total lipids ratio correlated with higher F2-isoprostane levels. Normal values in this healthy population can be used as the basis for future studies of disease mechanisms involving oxidative stress. 相似文献
77.
Vanciková Z Chlumecký V Sokol D Horáková D Hamsíková E Fucíková T Janatková I Ulcová-Gallová Z Stĕpán J Límanová Z Dvorák M Kocna P Sánchez D Tucková L Tlaskalová-Hogenová H 《Folia microbiologica》2002,47(6):753-758
The prevalence of celiac disease (CD) was determined in healthy blood donors and in high-risk groups of adults (a total of
1835 adults—randomly selected 1312 healthy blood donors, 102 patients with primary osteoporosis, 58 patients with autoimmune
diseases and 365 infertile women). It was calculated on the basis of a two-step serologic screening method—in the first step
IgA and IgG antigliadin antibodies (AGA) and IgA anti-γ-glutamyltransferase (‘transglutaminase’) antibodies (ATG) were estimated,
in the second step sera positive for IgA AGA and/or IgA ATG were examined for antiendomysial IgA (AEA) antibodies. Immunoenzymic
assay (ELISA) was used for determining of AGA and ATG antibodies; immunoflurescence method, performed on human umbilical cord
tissue, was used for assaying of AEA antibodies. Total serum IgA level in only IgG AGA positive subjects was measured by routine
turbidimetric method. 0.45% of healthy blood donors, 0.98% of osteoporotic patients, 2.7% of patients suffering from autoimmune
disease and 1.13% of women with infertility considered as immunologically mediated were found to be positive in both steps
of serologic screening (AGA and/or ATG and antiendomysium positive). The presumed high prevalence of seropositivity for CD
in apparently healthy Czech adult population was confirmed. In the high-risk groups, the prevalence of seropositivity for
CD was approximately 2–4 times higher than in healthy blood donors. The real prevalence of CD in the tested groups, however,
can be estimated after performing small intestinal biopsy in the seropositive patients. 相似文献
78.
Barbara Burwinkel Lluis Amat R. George F. Gray Nobutake Matsuo Koji Muroya Kuniaki Narisawa Ronald J. Sokol M. A. Vilaseca M. W. Kilimann 《Human genetics》1998,102(4):423-429
X-linked liver glycogenosis (XLG) resulting from phosphorylase kinase (Phk) deficiency is one of the most common forms of
glycogen storage disease. It is caused by mutations in the gene encoding the liver isoform of the Phk α subunit (PHKA2). In the present study, we address the issue of phenotypic and allelic heterogeneity in XLG. We have identified mutations
in seven male patients. One of these patients represents the variant biochemical phenotype, XLG subtype 2 (XLG2), where Phk
activity is low in liver but normal or even elevated in erythrocytes. He carries a K189E missense mutation, which adds to
the emerging evidence that XLG2 is associated with missense mutations clustering at a few sites. Two patients display clinical
phenotypes unusual for liver Phk deficiency, with dysfunction of the kidneys (proximal renal tubular acidosis) or of the nervous
system (seizures, delayed cognitive and speech abilities, peripheral sensory neuropathy), respectively, in addition to liver
glycogenosis. In the patient with kidney involvement, we have identified a missense mutation (P399S) and a trinucleotide deletion
(2858del3) leading to the replacement of two amino acids by one new residue (N953/L954I), and a missense mutation has also
been found in the patient with neurological symptoms (G1207W). These two cases demonstrate that PHKA2 mutations can also be associated with uncommon clinical phenotypes. Finally, in four typical XLG cases, we have identified
three truncating mutations (70insT, R352X, 567del22) and an in-frame deletion of eight well-conserved amino acids (2452del24).
Together, this study adds eight new mutations to the previously known complement of sixteen PHKA2 mutations. All known PHKA2 mutations but one are distinct, indicating pronounced allelic heterogeneity of X-linked liver glycogenosis with mutations
in the PHKA2 gene.
Received: 17 October 1997 / Accepted: 23 December 1997 相似文献
79.
F E Preston R J Sokol J S Lilleyman D A Winfield E K Blackburn 《BMJ (Clinical research ed.)》1978,1(6111):476-478
Six patients with various forms of leukaemia had neurological signs and symptoms associated with an extremely high white blood cell count and increased whole blood (but not plasma) viscosity. All were treated by leucapheresis with an Aminco Celltrifuge. Rapid and complete reversal of all symptoms occurred in three patients and partial recovery in one. One patient died shortly after leucapheresis and another (from cerebral intravascular coagulation) two days later. It is concluded that a cellular hyperviscosity syndrome may cause neurological dysfunction in patients with extremely high white cell counts, and that leucapheresis, in carefully selected patients, can be an effective method of treatment. 相似文献
80.