Cell Wall Polysaccharides of Callus and Suspension-Cultured Cells from Three Cellulose-less Mutants of Barley (Hordeum vulgare L.)

Cellulose contents of fragile culms of field-grown barley mutantswere about 20% of those of corresponding isogenic normal strains,while those of calluses and suspension-cultured cells of mutantswere closer to those of corresponding normal strains. Apparently,normal barley strains did not develop secondary cell walls inculture. ⁴Deceased 2-2-1993.     

Acetalation of 1,6-anhydro-1(6)-thio-D-glucitol

Acetalation of 1,6-anhydro-1(6)-thio-D-glucitol (1a) with acetone, formaldehyde, or benzaldehyde afforded 2,3:4,5-diacetals (2a, 2b, and 2c) whose structure, after desulfurization, was proved by mass spectrometry. Upon partial hydrolysis of 2a, one of the isopropylidene groups was split off, and the other migrated to O-3,O-4 to give 4b. In 4b, in the stable conformation, OH-2 occupies an equatorial position, whereas OH-5 is axially oriented. Accordingly, OH-2 reacts faster than OH-5 on methylation of 4b, giving 4e. Hydrolysis of the isopropylidene group of the 2,5-di-O-methyl derivative 4d and subsequent mesylation afforded the corresponding 3,4-di-O-mesyl compound 1c, which showed significant ulcerostatic activity.