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241.
Sofie Spatharis David Mouillot Michael Karydis George Tsirtsis 《Journal of experimental marine biology and ecology》2008,362(1):55-62
The relationship between biomass and species richness (SR) was studied along productivity gradients in coastal phytoplankton assemblages under the influence of terrestrial runoff. In particular, the investigation focused on (a) whether phytoplankton blooms affect the shape of the relationship, (b) whether the relationship is taxon specific (between dinoflagellates and diatoms), and (c) the potential mechanisms regulating the shape of the relationship. Analysis was based on phytoplankton and physicochemical data from six coastal areas in the Aegean Sea, Eastern Mediterranean, covering the whole range from oligotrophy to eutrophication. A SR-biomass relationship was not observed for the more oligotrophic areas, whereas hump-shaped curves were found in areas yielding higher maximum biomass values, corresponding to bloom events occurring mainly during winter. Furthermore, the observed hump-shaped relationships were taxon specific since they were more pronounced in the case of diatoms than dinoflagellates. It is hypothesized that SR at the leftmost part of the hump-shape is nutrient regulated, whereas at the rightmost part is limited by extreme abiotic stress occurring during the formation of blooms in coastal ecosystems. The results of this study suggest that a double stress mechanism related to abiotic factors may be responsible for the hump-shaped SR-biomass relationships occurring in coastal phytoplankton communities at various levels of productivity. 相似文献
242.
Schmuckli-Maurer J Shiels B Dobbelaere DA 《International journal for parasitology》2008,38(14):1705-1715
Theileria annulata inhabits the cytoplasm of bovine leukocytes where it can be found as a multinucleated schizont. The schizont is the pathogenic stage of the life cycle and by interfering with host signalling pathways, it induces unlimited host cell proliferation and protection against apoptosis. In the infected animal, the schizont differentiates to the merozoite life cycle stage in a process called merogony. This takes place within the host leukocyte, resulting in the production of merozoites that are subsequently released by leukocyte lysis. In established cultures of T. annulata-transformed cells, merogony does not spontaneously occur, but the process can be activated by a shift in temperature. In this study we show that chloramphenicol induces schizont differentiation in proliferating T. annulata-transformed cells. We demonstrate that chloramphenicol-induced merogony is inherently asynchronous and has a quantitative basis. The process is accompanied by the down-regulation of schizont-specific surface proteins, de novo expression of merozoite-specific markers such as Tamr1 and Tams1 and the morphological hallmarks of merogony. Chloramphenicol-induced parasite differentiation was found to be associated with diminished proliferation potential and extensive morphological changes of the host cell, including increased numbers of pseudopodia. Significantly, chloramphenicol treatment can accelerate merogony induced by elevated temperature, supporting postulation that the differentiation event is a stochastic process that can be manipulated to alter the outcome of parasitic infection. 相似文献
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Matthias B Van Hiel Pieter Van Wielendaele Liesbet Temmerman Sofie Van Soest Kristel Vuerinckx Roger Huybrechts Vanden Jozef Broeck Gert Simonet 《BMC molecular biology》2009,10(1):56
Background
To obtain reliable quantitative RT-PCR data, normalization relative to stable housekeeping genes is required. However, in practice, expression levels of 'typical' housekeeping genes have been found to vary between tissues and under different experimental conditions. To date, validation studies of reference genes in insects are extremely rare and have never been performed in locusts. In this study, putative housekeeping genes were identified in the desert locust, Schistocerca gregaria and two different software programs (geNorm and Normfinder) were applied to assess the stability of thesegenes. 相似文献245.
De Meyer T De Buyzere ML Langlois M Rietzschel ER Cassiman P De Bacquer D Van Oostveldt P De Backer GG Gillebert TC Van Criekinge W Bekaert S;Asklepios Investigators 《Aging cell》2008,7(5):700-705
Although telomere biology was revealed to play an important role in several hematopoietic disorders, its impact on the age-dependent dynamics of regular hematopoiesis is poorly understood. In vitro results suggest that particularly the erythropoietic capacity might be limited by critically short telomere length (TL). However, it remains unclear whether TL also affects erythropoiesis in healthy individuals in vivo. Therefore, we analyzed the associations between relevant hematopoietic parameters and peripheral blood leukocyte TL in the apparently healthy Asklepios study population, aged approximately 35-55 years (N > 2500). Our data indicate a clear positive, age and paternal age at birth adjusted, correlation between TL and red blood cell count, both in men (p < 0.001) and women (p = 0.011). This association was particularly significant in the older segment of the population (> 45 years old, both sexes: p = 0.003) and in younger men (p = 0.013), but not in younger women (p = 0.521). Further adjustment for known determinants in a general linear model revealed that peripheral blood leukocyte TL is most probably an independent predictor of red blood cell count (p < 0.001), suggesting that critical telomere shortening might also limit erythropoiesis in vivo. While negligible in a middle-aged population, the clinical consequences might be important in the elderly (e.g. in anemia of chronic disease). Further studies are required to confirm the impact of our results. 相似文献
246.
Van Bogaert IN De Maeseneire SL Develter D Soetaert W Vandamme EJ 《Journal of industrial microbiology & biotechnology》2008,35(10):1085-1092
The glyceraldehyde-3-phosphate dehydrogenase gene (GPD) of the sophorolipid producing yeast Candida bombicola was isolated using degenerated PCR and genome walking. The obtained 3,740 bp contain the 1,008 bases of the coding sequence and 1,613 and 783 bp of the upstream and downstream regions, respectively. The corresponding protein shows high homology to the other known GPD genes and is 74% identical to the gyceraldehyde-3-phosphate dehydrogenase of Yarrowia lipolytica. The particular interest in the C. bombicola GPD gene sequence originates from the potential use of its promoter for high and constitutive expression of homologous and heterologous genes. Southern blot analysis did not give any indication for the presence of multiple GPD genes and it can therefore be expected that the promoter can be used for efficient and high expression. This hypothesis was further confirmed by the biased codon usage in the GPD gene. GDP promoter fragments of different lengths were used to construct hygromycin resistance cassettes. The constructs were used for the transformation of C. bombicola and all of them, even the ones with only 190 bp of the GPD promoter, were able to render the cells resistant to hygromycin. The efficacy of a short GPD promoter can be a convenient characteristic for the construction of compact expression cassettes or vectors for C. bombicola. The GenBank accession number of the sequence described in this article is EU315245. 相似文献
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249.
Ola Svensson Johanna Gräns Malin C. Celander Jonathan Havenhand Erica H. Leder Kai Lindström Sofie Schöld Cock van Oosterhout Charlotta Kvarnemo 《Evolution; international journal of organic evolution》2017,71(10):2510-2521
The distributions of species are not only determined by where they can survive – they must also be able to reproduce. Although immigrant inviability is a well‐established concept, the fact that immigrants also need to be able to effectively reproduce in foreign environments has not been fully appreciated in the study of adaptive divergence and speciation. Fertilization and reproduction are sensitive life‐history stages that could be detrimentally affected for immigrants in non‐native habitats. We propose that “immigrant reproductive dysfunction” is a hitherto overlooked aspect of reproductive isolation caused by natural selection on immigrants. This idea is supported by results from experiments on an externally fertilizing fish (sand goby, Pomatoschistus minutus). Growth and condition of adults were not affected by non‐native salinity whereas males spawning as immigrants had lower sperm motility and hatching success than residents. We interpret these results as evidence for local adaptation or acclimation of sperm, and possibly also components of paternal care. The resulting loss in fitness, which we call “immigrant reproductive dysfunction,” has the potential to reduce gene flow between populations with locally adapted reproduction, and it may play a role in species distributions and speciation. 相似文献
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