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排序方式: 共有75条查询结果,搜索用时 15 毫秒
51.
JP Valde LG Lawson A Lindberg JF Agger H Saloniemi O Østerås 《Acta veterinaria Scandinavica》2004,45(4):201-210
Data from the national dairy cow recording systems during 1997 were used to calculate lactation-specific cumulative risk of mastitis treatments and cumulative risk of removal from the herds in Denmark, Finland Norway and Sweden. Sweden had the lowest risk of recorded mastitis treatments during 305 days of lactation and Norway had the highest risk. The incidence risk of recorded mastitis treatments during 305 days of lactation in Denmark, Finland, Norway and Sweden was 0.177, 0.139, 0.215 and 0.127 for first parity cows and 0.228, 0.215, 0.358 and 0.204 for parities higher than three, respectively. The risk of a first parity cow being treated for mastitis was almost 3 times higher at calving in Norway than in Sweden. The period with the highest risk for mastitis treatments was from 2 days before calving until 14 days after calving and the highest risk for removal was from calving to 10 days after calving in all countries.The study clearly demonstrated differences in bovine mastitis treatment patterns among the Nordic countries. The most important findings were the differences in treatment risks during different lactations within each country, as well as differences in strategies with respect to the time during lactation mastitis was treated. 相似文献
52.
SE?Aleshin AV?Timofeev MV?Khoretonenko LG?Zakharova GV?Pashvykina JR?Stephenson AM?Shneider AD?AltsteinEmail author 《BMC microbiology》2005,5(1):45
Background
Heterologous prime-boost immunization protocols using different gene expression systems have proven to be successful tools in protecting against various diseases in experimental animal models. The main reason for using this approach is to exploit the ability of expression cassettes to prime or boost the immune system in different ways during vaccination procedures. The purpose of the project was to study the ability of recombinant vaccinia virus (VV) and bacterial plasmid, both carrying the NS1 gene from tick-borne encephalitis (TBE) virus under the control of different promoters, to protect mice against lethal challenge using a heterologous prime-boost vaccination protocol. 相似文献53.
The polymerization of actin: II. how nonfilamentous actin becomes nonrandomly distributed in sperm: evidence for the association of this actin with membranes 总被引:16,自引:13,他引:3
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LG Tilney 《The Journal of cell biology》1976,69(1):51-72
At an early stage in spermiogenesis the acrosomal vacuole and other organelles including ribosomes are located at the basal end of the cell. From here actin must be transported to its future location at the anterior end of the cell. At no stage, in the accumulation of actin in the periacrosomal region is the actin sequested in a membrane-bounded compartment such as a vacuole or vesicle. Since filaments are not present in the periacrsomoal region during the accumulation of the actin even though the fixation of these cells is sufficiently good to distinguish actin filaments in thin section, the actin must accumulate in the nonfilamentous state. 相似文献
54.
Thaís?Furtado?Nani James?C.?Schnable Jacob?D.?Washburn Patrice?Albert Welison?Andrade?Pereira Fausto?Souza?Sobrinho James?A.?Birchler Vania?Helena?TechioEmail author 《Molecular biology reports》2018,45(2):109-118
Repetitive DNA sequences have been widely used in cytogenetic analyses. The use of gene sequences with a low-copy-number, however, is little explored especially in plants. To date, the karyotype details in Brachiaria spp. are limited to the location of rDNA sites. The challenge lies in developing new probes based on incomplete sequencing data for the genus or complete sequencing of related species, since there are no model species with a sequenced genome in Brachiaria spp. The present study aimed at the physical location of conserved genes in chromosomes of Brachiaria ruziziensis, Brachiaria brizantha, and Brachiaria decumbens using RNAseq data, as well as sequences of Setaria italica and Sorghum bicolor through the fluorescent in situ hybridization technique. Five out of approximately 90 selected sequences generated clusters in the chromosomes of the species of Brachiaria studied. We identified genes in synteny with 5S and 45S rDNA sites, which contributed to the identification of chromosome pairs carrying these genes. In some cases, the species of Brachiaria evaluated had syntenic segments conserved across the chromosomes. The use of genomic sequencing data is essential for the enhancement of cytogenetic analyses. 相似文献
55.
In this paper, we tested four hypotheses relative to edge and shape effects on ant communities: (i) forest edges have lower species richness than the remnant core; (ii) species richness increases with distance from the edge; (iii) irregularly shaped remnants have lower species richness than more regular remnants; (iv) there is a higher similarity of species composition between edge and core in irregular than in regular remnants. We sampled litter ant communities on the edge and core of ten remnants, in Viçosa, Minas Gerais, Brazil. Species richness was larger at the forest core than at the edges, although did not increase with distance from the edge. Species richness did not vary with shape complexity. The similarity of species composition between edge and core showed a decreasing trend with remnant area, and did not vary with shape complexity. The observed differences of species richness between forest core and edge may be due to higher harshness of edges, caused by environmental changes. The absence of relationship between species richness and distance from the edge might indicate the range of edge effects, which would be smaller than the smallest distance of core sampled. Therefore, edges would affect litter-dwelling ant species richness in a distance smaller than 50 m. The observation of species composition allowed us to notice an effect of fragmentation that would not be noticed if we were considering only species richness. Edge may serve as step to generalist species, which may use it to colonise forest remnants. Furthermore, small remnants are more colonisation-prone by such species, and have a more homogeneous species composition than large remnants. 相似文献
56.
Alícia Dorneles Dornelles Louise Lapagesse de Camargo Pinto Ana Carolina de Paula Carlos Eduardo Steiner Charles Marques Louren?o Chong Ae Kim Dafne Dain Gandelman Horovitz Erlane Marques Ribeiro Eugênia Ribeiro Valadares Isabela Goulart Isabel C. Neves de Souza Jo?o Ivanildo da Costa Neri Luiz Carlos Santana-da-Silva Luiz Roberto Silva Márcia Ribeiro Ruy Pires de Oliveira Sobrinho Roberto Giugliani Ida Vanessa Doederlein Schwartz 《Genetics and molecular biology》2014,37(1):23-29
Mucopolysaccharidosis type I (MPS I) is a rare lysosomal disorder caused by deficiency of alpha-L-iduronidase. Few clinical trials have assessed the effect of enzyme replacement therapy (ERT) for this condition. We conducted an exploratory, open-label, non-randomized, multicenter cohort study of patients with MPS I. Data were collected from questionnaires completed by attending physicians at the time of diagnosis (T1; n = 34) and at a median time of 2.5 years later (T2; n = 24/34). The 24 patients for whom data were available at T2 were allocated into groups: A, no ERT (9 patients; median age at T1 = 36 months; 6 with severe phenotype); B, on ERT (15 patients; median age at T1 = 33 months; 4 with severe phenotype). For all variables in which there was no between-group difference at baseline, a delta of ≥ ± 20% was considered clinically relevant. The following clinically relevant differences were identified in group B in T2: lower rates of mortality and reported hospitalization for respiratory infection; lower frequency of hepatosplenomegaly; increased reported rates of obstructive sleep apnea syndrome and hearing loss; and stabilization of gibbus deformity. These changes could be due to the effect of ERT or of other therapies which have also been found more frequently in group B. Our findings suggest MPS I patients on ERT also receive a better overall care. ERT may have a positive effect on respiratory morbidity and overall mortality in patients with MPS I. Additional studies focusing on these outcomes and on other therapies should be performed. 相似文献
57.
Roberta M. Crivelaro Roberto Thiesen Marcela Aldrovani Paloma E.S. Silva Alexandre A.F. Barros Sobrinho Paola C. Moraes 《Journal of fish biology》2019,94(5):823-827
Through the analysis of behavioural changes, this study demonstrates that methadone has behavioural, but not analgesic, effects on Oreochromis niloticus. It provides information that suggests the drug has sedative abilities, as the recovery time was shorter in the fish receiving methadone. Future research, with different doses and stimuli, is required to provide more information about analgesia. 相似文献
58.
Emanoel Gomes de Moura Sheilla Silva Serpa José Geraldo Donizetti dos Santos João Reis Salgado Costa Sobrinho Alana das Chagas Ferreira Aguiar 《Plant and Soil》2010,335(1-2):363-371
In the SE periphery of Brazilian Amazonia, low-input agriculture systems on sandy loam soils have very low nutrient use efficiency. In a low-input alley cropping system, we measured residue decomposition dynamics and the yield and nutrient uptake of a maize crop associated with the following treatments: Clitoria + Pigeon pea; Acacia + Pigeon pea; Leucaena + Clitoria; Leucaena + Acacia, Leucaena + Pigeon pea and no residue input (control). The acacia treatments provided better soil coverage throughout the whole corn cycle. Potassium was released faster than nitrogen from the residues; N concentration in corn leaves in the residue treatments were below critical levels. The Leucaena + Acacia treatment was the most effective in increasing post-tasseling N and K assimilation and K use efficiency. This resulted in corn productivity 3.5 times greater (7.3 Mg ha?1) than the control without residue application. In the Amazonian sandy loam soils, which are susceptible to hardsetting and nutrient leaching, efficient N and K use should be priorities for soil management. Although no-till alley cropping of leguminous trees constitutes an important option for low-input farming, its efficiency depends on using a mixture of residues that keeps soil covered and have high rates of both N and K release during the entire crop cycle. 相似文献
59.
Ana Carolina Callegario Pereira Nelson Moura Brasil do Amaral Sobrinho Alfredo Tolón-Becerra Marcio Osvaldo Lima Magalhães Nelson Mazur Xavier Lastra-Bravo 《Soil & Sediment Contamination》2013,22(4):376-389
The objective of this study was to evaluate the phytostabilization of two substrates contaminated with heavy metals from excavations of the ore courtyard at the port of Itaguai, Brazil. Initially, an inventory of tree species located near the study area was performed to select species for phytostabilization. The species Cordia africana exhibited deeper roots, a larger diameter at chest height (DCH) and crown diameter, as well as higher concentrations of Zn, Cd, and Pb in the trunk, bark, and roots compared to the other investigated species. The tolerance of the selected species to the metals Zn, Cd, and Pb was subsequently assessed through a greenhouse test. The substrates used in the experiment also obtained from excavations at the ore courtyard and showed different levels of heavy metals, indicating either low contamination (Substrate 1) or high contamination (Substrate 2). Alkaline industrial waste steel slag (SS) was used as an amendment to reduce the solubility of heavy metals. Application of the amendment agent to the substrates reduced the bioavailability of heavy metals, favoring the growth of C. africana. This species presents potential for use in phytostabilization programs due to its tolerance for heavy metals and the observed higher accumulation of these metals in the roots and especially the trunk of this species compared to other vegetal parts. 相似文献
60.
Basil P Hubbard Christine Loh Ana P Gomes Jun Li Quinn Lu Taylor LG Doyle Jeremy S Disch Sean M Armour James L Ellis George P Vlasuk David A Sinclair 《Cell cycle (Georgetown, Tex.)》2013,12(14):2233-2240
SIRT1 is an NAD+-dependent deacetylase that counteracts multiple disease states associated with aging and may underlie some of the health benefits of calorie restriction. Understanding how SIRT1 is regulated in vivo could therefore lead to new strategies to treat age-related diseases. SIRT1 forms a stable complex with DBC1, an endogenous inhibitor. Little is known regarding the biochemical nature of SIRT1-DBC1 complex formation, how it is regulated and whether or not it is possible to block this interaction pharmacologically. In this study, we show that critical residues within the catalytic core of SIRT1 mediate binding to DBC1 via its N-terminal region, and that several carboxamide SIRT1 inhibitors, including EX-527, can completely block this interaction. We identify two acetylation sites on DBC1 that regulate its ability to bind SIRT1 and suppress its activity. Furthermore, we show that DBC1 itself is a substrate for SIRT1. Surprisingly, the effect of EX-527 on SIRT1-DBC1 binding is independent of DBC1 acetylation. Together, these data show that protein acetylation serves as an endogenous regulatory mechanism for SIRT1-DBC1 binding and illuminate a new path to developing small-molecule modulators of SIRT1. 相似文献