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61.
A. P. Bonartsev S. G. Yakovlev E. V. Filatova G. M. Soboleva T. K. Makhina G. A. Bonartseva K. V. Shaitan V. O. Popov M. P. Kirpichnikov 《Biochemistry (Moscow) Supplemental Series B: Biomedical Chemistry》2012,6(1):42-47
The development of sustained release formulations based on biodegradable polymers is a promising trend in modern pharmacology.
Polyhydroxyalkanoates (PHA) attract increasing attention due to their biodegradability and high biocompatibility, which make
them suitable for the development of novel drug dosage forms. We have produced poly(3-hydroxybutyrate) (PHB)-based microspheres
loaded with the antitumor drug paclitaxel and investigated morphology, drug release kinetics and the effect of these microspheres
on tumor cells in vitro. The data on the kinetics of drug release, biocompatibility and biological activity of the biopolymer
microspheres in vitro have demonstrated that the studied system of prolonged drug release had lower toxicity and higher efficiency
compared to the traditional dosage forms of paclitaxel. 相似文献
62.
63.
Polymorphism of genes of the antioxidant system in the development of predispositions to lung cancer
R. A. Titov V. I. Minina O. A. Soboleva A. V. Ryzhkova Yu. E. Kulemin E. N. Voronina 《Russian Journal of Genetics》2017,53(8):903-909
We analyzed the polymorphic loci in the genes of the antioxidant system enzymes, such as GSTP1 (313A>G and 341C>T), MnSOD (47С>Т), GPx1 (599C>T), and CAT (–262C>>T), among 497 residents of Kemerovo oblast (Western Siberia, Russia). The analysis of the single-locus effects demonstrated a significant protective effect of the major C allele in the GPx1 (599C>T) locus. The MDR analysis of the gene-gene interactions showed that the GPx1 and the CAT genes work in close cooperation and mutually reinforce the risk of development of squamous cell lung cancer among the inhabitants of the industrial region. 相似文献
64.
Shorten PR Vetharaniam I Soboleva TK Wake GC Davis SR 《Journal of theoretical biology》2002,218(4):521-530
The effects of milking frequency on milk production is a key question for the dairy industry. Milk production is related to the number of active alveoli in the mammary gland and movement between active and quiescent alveolar pools is influenced by the milking frequency. In this paper, we analyse a mechanistic model based on known biological inputs that describes the effect of milking frequency on the alveolar composition of the mammary gland. It is shown that the model can qualitatively reproduce the correct alveolar dynamics. We also investigate the model robustness and parameter sensitivity. Additionally, by making the plausible assumption that the senescence rate of alveoli is proportional to the number of quiescent alveoli present, we obtain an analytical solution requiring periodic resetting. 相似文献
65.
Formation kinetics, specificity, and analytical potential of polyclonal antibodies raised in rabbits against BSA conjugates of carboxymethyloxime-zearalanone (CMO-ZAN) and carboxymethyloxime-zearalenone (CMO-ZEN). Preparation of the conjugates involved conversion of CMO-ZAN and CMO-ZEN into activated esters and carbodiimide condensation. Two versions of a group-specific enzyme immunoassay (for zearalenone/alpha-zearalenone and zearalanol/alpha-zearalanol) based on heterologous combination of solid-phase antigens are described (sensitivity, 0.01 ng/ml). 相似文献
66.
67.
Burkin AA Konenenko GP Soboleva NA Zotova EV 《Prikladnaia biokhimiia i mikrobiologiia》2000,36(3):328-335
Zearalenone-6'-carboxymethyloxime was synthesized, and its conjugates with albumins and gelatin were prepared. Polyclonal rabbit antibodies against the conjugate with bovine serum albumin were shown to be highly specific to zearalenone and to have a lower cross-reactivity toward its structural analogues (alpha-zearalenol--28%, beta-zearalenol--6%, zearalanone--12%, and alpha-zearalanol--5%). The sensitivity of enzyme immunoassay using gelatin-based immobilized conjugates for determination of zearalenone in solutions was 1 ng/ml, and this allowed us to determine this substance in feed at a threshold concentration of 200 micrograms/kg. 相似文献
68.
G. P. Kononenko A. A. Burkin E. V. Zotova N. A. Soboleva 《Applied Biochemistry and Microbiology》2000,36(2):177-180
Ochratoxin A was quantitatively monitored in grain extracts by indirect solid-phase enzyme immunoassay with the use of an
immobilized conjugate of the toxin with gelatin and polyclonal rabbit antibodies raised against the ochratoxin A-BSA conjugate.
This monitoring found that 1.7 to 18.5% of the samples were contaminated with the toxin at a concentration of 25.9–291.7 μg/kg.
An analysis of forage grain found ochratoxin A at concentrations of 440-3250 μg/kg. 相似文献
69.
Aflatoxin B2a (AB2a), aflatoxin G2a (AG2a), and hemiacetal of sterigmatocystin have been shown to form immunoreactive conjugates with albumin. The conjugates were formed following incubation of solution mixtures at room temperature for 1 h, as demonstrated by spectrophotometry and enzyme immunoassay. Anti-AB2a antibodies reacted with AB2a, aflatoxin B1, and aflatoxin AB2 (100, 8.8, and 5.9%, respectively); a similar result was obtained for anti-AG2a antibodies reacting with AG2a, aflatoxin G1, and aflatoxin AG@2 (100, 2.5, and < 1.0%, respectively). Binding of anti-AB2a and anti-AG2a antibodies to solid-phase conjugates of AB2a or AG2a exhibited similar analytical characteristics. 相似文献
70.
DN Akongyuure S Amisah TK Agyemang RE Edziyie 《African Journal of Aquatic Science》2017,42(2):143-154
Fishery characteristics and livelihood status of fishers at Tono Reservoir, Ghana, were investigated between January 2015 and June 2016. Data on fisher demography, fishing gears, fishing methods, perceptions of the state of fish stocks, management practices, income and consumption of fishers were obtained through structured interviews. Censuses of fishers and fishing gears were conducted through direct observation and counts. The population of fishers was 950 and the majority (74%) of the sampled respondents fell within the ages of 24–41 years. Gillnet, cast net, trap and hook and line were the four main gears utilised. Illegal methods of fishing observed included the use of mosquito nets (nets with mesh <1.0 cm) and the use of brewer’s waste (pito mash) as bait. Brycinus nurse, Synodontis spp., Parailia spiniserrata and Chrysichthys spp. were perceived to have disappeared from the reservoir. The fishers were unaware of the existence of any fisheries regulations, hence there was no adherence to management practices. Their daily income was derived mainly from fishing. The incidence of poverty among fishers was low (8%). The Tono Reservoir has a great potential for supporting livelihood if it is properly managed. 相似文献