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271.
V N Timerbaev D M Zubairov S V Kiselev S V Kirshin I V Soboleva 《Biokhimii?a (Moscow, Russia)》1992,57(1):77-90
The binding of 125I-labeled prothrombin fragment I. prethrombin I and alpha-thrombin to native and papain-treated tissue thromboplastin in the presence of CaCl2 of EDTA was studied. The experimental curves plotted in the Scatchard coordinates testify to the presence in thromboplastin of two types of fragment I binding sites: those with a high (Kd = 7.6 x 10(-6) M) and moderate (Kd = 1.3 x 10(-8) M) binding affinity. The parameters of fragment I binding and their changes reproduced, for the most part, the mode of prothrombin binding observed in previous studies. The experimental results provide indirect evidence in favour of a hydrophobic role of Ca(2+)-dependent binding of prothrombin fragment I to thromboplastin. The binding of prethrombin I was nonspecific and Ca(2+)-independent, whereas alpha-thrombin showed a relatively high level of nonspecific electrostatic binding which was competitively inhibited by Ca2+. Thromboplastin proteins interacted (both directly and in a Ca(2+)-independent fashion) with all the prothrombin derivatives under study. 相似文献
272.
G P Kononenko A A Burkin E B Zotova N A Soboleva 《Prikladnaia biokhimiia i mikrobiologiia》1999,35(2):206-211
A varriant of competitive ELISA, making it possible to detect fumonisin B1 (up to 0.4 ng/well) was developed, based on the use of specific polyclonal antibodies obtained by immunization of rabbits with a horseradish peroxidase conjugate of fumonisin B1. The minimum concentration of fumonisin B in water-acetonitrile extracts of corn grain, dry corn products, and feeds, detected by the ELISA version developed is 0.2 mg/kg. 相似文献
273.
274.
T. L. Karaseva B. A. Lobasyuk S. G. Soboleva E. A. Kostenko S. A. Andronati 《Neurophysiology》2000,32(1):8-11
The neuropharmacological profile of 4-phenyl-1-[4-(2-naphtalimido)butyl]-piperazine (PNBP), a compound that possesses a high
affinity for the serotonin receptors of 1A-type (5-HT1A) and lacks an anxiolytic action, has been studied. Intracerebral administration of PNBP to rats through implanted cannulae
into the hippocampal region resulted in no substantial behavioral changes during “open field” and “conflict situation” tests,
as compared with those of control animals. At the same time, the behavioral effects of intraperitoneal administration of 10
mg/kg buspirone were completely abolished if buspirone had been jointly administered with 10 mg/kg PNBP. Moreover, combined
application of 0.3 mg/kg PNBP and 0.3 mg/kg 8-OH-DPAT, an agonist of 5-HT1A receptors, almost completely abolished the components of “serotonin syndrome” (prone position and stamping of the forepaws)
in animals under study. These findings allowed us to conclude that PNBP has the properties of a competitive antagonist of
buspirone and 8-OH-DPAT. 相似文献
275.
S M Zhenodarova O A Smolianinova I A Soboleva M I Khabarova 《Bioorganicheskaia khimiia》1987,13(8):1023-1030
Immobilized guanyl-specific ribonucleases from Aspergillus clavatus (C2), A. oryzae (T1), and Bacillus intermedius 7P (Bi) have been used for preparative synthesis of ten trinucleoside diphosphates, three tetra- and one pentanucleotide having the only guanylic acid residue at the 5'-end. The nucleotide sequence of the oligonucleotide synthesised determined the choice of the ribonuclease. 相似文献
276.
L F Levina E G Volina G L Soboleva G A Arutiunova 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1983,(8):27-31
The phospholipase activity of leptospires cultivated on serum-lecithin agar has been studied. Two zones of changes in the medium have been found to appear around the colonies of saprophytic Leptospira strains: transparent (5.25 +/- 2.09 mm wide) and turbid (6.90 +/- +/- 1.46 mm wide), which is linked with the production of phospholipases A and C. Only a single clear zone is formed around the colonies of pathogenic strains due to the production of phospholipase A. At the same time virulent Leptospira strains show greater phospholipase activity (the zones are 6.0 +/- 1.2 mm wide) than avirulent strains (the zones are 1.6 +/- +/- 0.04 mm wide). 相似文献
277.
We postulated that the biogeographical history of South-east Asia contributed to extensive admixture during Pleistocene low sea levels of genetic groups of an obligate freshwater fish (the river catfish, Hemibagrus nemurus) isolated during periods of high sea levels. During Pleistocene glacial maxima, the sea level was lower than at present and the islands of the Sunda shelf (Sumatra, Borneo and Java) and the Asian mainland were connected by lowlands traversed by rivers. Restriction fragment length polymorphisms in mitochondrial DNA were documented for 140 putative H. nemurus analysed from 13 sampling sites resulting in the definition of 35 haplotypes. The high level of haplotype differentiation (mean P × 100 = 2.22, SD = 1.33) indicates that the subdivision of the ancestral H. netnurus group was extensive and probably occurred early in the Pleistocene. The occurrence of some genetically divergent groups of the H. netnurus complex occurring in sympatry in widely separated locations supports the proposition that low sea levels aided the dispersion and mingling of genetic groups. Based on both genetic and morphological evidence, the main H. nemurus line gave rise to three regional groups: (1) a morphologically distinct ‘Indochinese’ group composed of two mtDNA clades overlapping in east peninsular Malaysia; (2) a ‘Sundaic’ group composed of various lineages of differing morphology and genetic identity; (3) a genetically distinct ‘Sarawak’ group in west Borneo, similar in morphology to the ‘Sundaic’ and ‘Indochinese’ groups, but including a small, golden colour morph as a distinct dade. The morphologically similar Sundaic forms from west Java, Sumatra and west Borneo show some degree of genetic divergence, but their phylogenetic relationships are poorly resolved. The most genetically and morphologically distinct Sundaic dade, assigned to H. hoevenii, colonized the Kapuas river (west Borneo), east Sumatra and south peninsular Malaysia. Contrary to our original hypothesis and present biogeographical theory, little exchange of genetic groups has apparently occurred between the mainland and the Sunda Islands during recent glaciations. 相似文献