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Hyo Je Cho Kyungsun Kim Seo Yean Sohn Ha Yeon Cho Kyung Jin Kim Myung Hee Kim Dockyu Kim Eungbin Kim Beom Sik Kang 《The Journal of biological chemistry》2010,285(45):34643-34652
A meta-cleavage pathway for the aerobic degradation of aromatic hydrocarbons is catalyzed by extradiol dioxygenases via a two-step mechanism: catechol substrate binding and dioxygen incorporation. The binding of substrate triggers the release of water, thereby opening a coordination site for molecular oxygen. The crystal structures of AkbC, a type I extradiol dioxygenase, and the enzyme substrate (3-methylcatechol) complex revealed the substrate binding process of extradiol dioxygenase. AkbC is composed of an N-domain and an active C-domain, which contains iron coordinated by a 2-His-1-carboxylate facial triad motif. The C-domain includes a β-hairpin structure and a C-terminal tail. In substrate-bound AkbC, 3-methylcatechol interacts with the iron via a single hydroxyl group, which represents an intermediate stage in the substrate binding process. Structure-based mutagenesis revealed that the C-terminal tail and β-hairpin form part of the substrate binding pocket that is responsible for substrate specificity by blocking substrate entry. Once a substrate enters the active site, these structural elements also play a role in the correct positioning of the substrate. Based on the results presented here, a putative substrate binding mechanism is proposed. 相似文献
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The effect of caffeine on nitrosoguanidine-induced mutagenesis ofSalmonella typhimurium & nd its P22 and L phages was studied. The detected mutations included phage “clear” mutations, reversions of phage “amber”
mutation, and prototrophic reversions of thehis
−
auxotroph ofSalmonella typhimurium. Neither therecA mutation of the host nor theerf mutation of the phage genome were found to affect the nitrosoguanidine-induced mutagenesis of the phage during vegetative
growth. Beginning with a concentration of 0.2 mg/ml, caffeine decreased the frequency of mutants by 30–60%, attaining a maximum
effect at 1.5 mg/ml and retaining this effect even at higher concentrations. A similar antimutagenic effeot was observed with
the mutagenesis of the host cells. The nitrosoguanidine-induced mutagenesis does not seem to be related to the function of
therecA cell gene or theerf phage gene. The mechanism of mutagenesis by nitrosoguanidine probably has two components, one of them caffeine sensitive,
the other caffeine-resistant. 相似文献
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Thymineless death (TLD) as well as deoxyribosideless death (DRLD) can be observed inLactobacillus acidophilus R-26 during growth in media lacking thymine or deoxyriboside respectively. Both phenomena exhibit the same interval of lage
period (2–3 h) but the rate of inactivation is 2–3 times faster in TLD. Transfer experiments show that inactivation of bacterial
reproduction is accelerated immediately if—DR medium is replaced by—T one. In the opposite case the deceleration of the inactivation
rate does not appear immediately but after a 1–2 h lag period, in which no changes in the number of viable bacteria can be
observed. Our results suggested that the accumulation of deoxyriboside compounds has no causal role in the inactivation of
bacterial reproduction. However, the presence of deoxyribosides can accelerate the process of inactivation. 相似文献
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