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901.
902.
N.K. Kochetkov O.S. Chizhov V.I. Kadentsev G.P. Smirnova I.G. Zhukova 《Carbohydrate research》1973,27(1):5-10
The fragmentation pattern in electron-impact mass spectrometry has been established for the peracetylated methyl ester methyl glycoside derivative of N-acetylneuraminic acid. The resulting, data allow the interpretation of the mass spectrum of the corresponding derivative of a new sialic acid isolated from the starfish Distolasterias nipon which is shown to be 8-O-methyl-N-acetylneuraminic acid. 相似文献
903.
The conveyer hypothesis is based on the fact that because of clone predetermination, antibody production takes place in an
organism without the presence of antigen as a result of natural cell differentiation. Soluble antigen is an analogue of a
specific mitogen which gives rise to reproduction mainly of cells carrying on their surface the immunoglobulin receptors to
the given antigen. The mathematical model of the conveyer hypothesis takes into account the initial conditions, among them
the background level of antibody-producing cells before injection of a soluble antigen, migration of precursor cells in the
draining lymphoid organ, and the rate of precursor differentiation, including the rate of the change of the immunoglobulin
receptor number on the cell surface. Changes of antigen concentration in blood determine the intensity of precursor proliferation.
Comparison of real experiments (intraperitoneal injection of capsular antigen ofPasteurella pestis into inbred mice) with calculations done on the basis of the developed mathematical model shows a definite qualitative resemblance
with the kinetics of antibody-producing cells and free antibodies as well as with the decrease of free antigen concentration
in blood. In spite of some differences between model experiments and real experiments the conveyer hypothesis and its mathematical
model appear suitable for describing the primary immune response of mice immunized with low doses of capsular antigen ofPasteurella pestis. 相似文献
904.
M N Smirnova E A Bazanova I M Liampert 《Biulleten' eksperimental'no? biologii i meditsiny》1976,81(4):465-467
A study was made in the autologous system of the cytotoxic action of immune lymphocytes on the "adherent" cells (macrophages) of the lymph nodes of guinea pigs in delayed hypersensitivity (DH) to the streptococcus antigens and tuberculoproteins. Death of a considerable number of the "adherent" cells in cultivation of a suspension of cells of the lymph nodes of animals sensitized with the culture of streptococcus or BCG in the presence of specific antigen (thermo-stable streptococcus fraction or tuberculin, respectively) alone. Detection of death of the "adherent" cells in the autologous system can be used as one of specific and sensitive tests in studying the DH. 相似文献
905.
The effect of pH on the expression of the cea gene encoding colicin EI in Escherichia coli was investigated by measuring the beta-galactosidase activity in the UV-irradiated growing cells carrying the cea-lacZ fusion. Maximum activity was observed at pH 7, and inhibition of expression was observed at pH 6 and pH 8. Treatment of the irradiated cells with 50-mM acetate increased inhibition at pH 6.0-7.5. No correlation between cea expression and the rate of cell growth was observed at different pH levels. Preliminary treatment with acetate at pH 7 reduced the expression of the recA gene, which participates in the regulation of the cea gene to 33% in irradiated cells and to 25% in nonirradiated cells. 相似文献
906.
N I Smirnova N M Mestechkina V D Shcherbukhin 《Prikladnaia biokhimiia i mikrobiologiia》2001,37(3):332-337
Water-soluble glucomannans from roots of Eremurus iae and E. zangezuricus were studied. These polysaccharides were shown to contain 28.8; 69.0, and 2.2% (E. iae) and 22.6; 74.8, and 2.6% (E. zangezuricus) of D-glucose, D-mannose and acetyl groups, respectively. Their IR spectra were identical and revealed the presence of 1,4-beta-glycosidic bonds and ester carbonyl groups. 13C-NMR spectroscopy revealed both polysaccharides to be linear partially acetylated 1,4-beta-D-glucomannans. Acetyl groups substituted C-2- and C-3-hydroxyls of mannopyranose residues. Comparison of 13C-NMR data and the results of correlation analysis enables a conclusion to be made that acetyl groups can substitute no more than one OH-group in the mannopyranosyl residue. [alpha]D = -34.0 degrees, [eta] and molecular weights (MW) for E. iae polysaccharide were determined to be -34.0, 6.5 dl/g, and 265.5 kDa, respectively, and for E. zangezuricus polysaccharide -38.2, 5.4 dl/g, and 233.5 kDa, respectively. A correlation between intrinsic viscosities of polysaccharides and their molecular masses determined by HPLC was revealed. 相似文献
907.
Light-induced structural changes in photosynthetic reaction centers from Rhodobacter sphaeroides were investigated using two approaches. Cu2+ was used as a paramagnetic structural probe. The EPR spectrum of Cu2+ incorporated into the metal-depleted reaction centers was affected by 1,10-phenanthroline, an electron transfer inhibitor substituting QB, which suggests a localization of Cu2+ in a vicinity of the Q
B
–
site. However, the spectrum was not influenced by low temperature (77 K) illumination of the sample which suggests that the copper ion position is not exactly the same as that of the iron ion. Freezing the reaction centers under illumination in the presence of potassium ferricyanide and 1,10-phenanthroline caused a change in the shape of the Cu2+ EPR spectrum in comparison to that of a sample frozen in darkness. These data indicate a change of the Cu2+ ligand symmetry owing to light-induced structural changes which are probably located near the acceptor side of the reaction center. Partial trypsinolysis of reaction centers was also used to locate the structural changes. Trypsin treatment in the dark and under illumination resulted in different peptide patterns as detected by gel electrophoresis and reverse-phase high-performance liquid chromatography. Partial amino-acid sequence analysis of a number of peptides, characteristic of either light- or dark-treated reaction centers, showed that they originated from the acceptor sides of the H and M subunits. The occurrence of light-induced structural differences in the H-subunit is consistent with the suggestion that it may be involved in regulating electron transfer in this part of the reaction center. 相似文献
908.
Atko Viru Livian Laaneots Kalle Karelson Tamara Smirnova Mehis Viru 《European journal of applied physiology and occupational physiology》1998,77(5):401-408
The dependence of exercise-induced hormone responses on sexual maturation was tested in a 3-year longitudinal experiment
on 34 girls (aged 11–12 years at the beginning). Sexual maturation was evaluated by Tanners five-stage scale. Children cycled
for 20-min at 60% maximal oxygen uptake once a year. Cortisol, insulin, growth hormone, β-oestradiol, progesterone and testosterone
concentrations in venous blood were determined by radioimmunoassay procedures. Basal concentrations of growth hormone increased
and of cortisol decreased when breast stage III was reached. Reaching breast stage IV was associated with an increase in basal
concentrations of β-oestradiol, progesterone and testosterone. The exercise induced significant increases in concentrations
of cortisol, growth hormone and β-oestradiol and a decrease in insulin concentration. At breast stage III the increase in
cortisol concentration was to a lower level [467 (SEM 42) vs 567 (SEM 46)nmol · l−1] and growth hormone concentration to a higher level [29.4 (SEM 0.5) vs 12.8 (SEM 0.4)ng · ml−1], while the fall in insulin concentration was less pronounced [postexercise level 10.6 (SEM 0.9) vs 7.8 (SEM 0.8)mU · l−1] than in stage II. The magnitude of the cortisol response was reduced in the last stage of breast development (+42.1% vs
+55.5% at stage II, +66.2% at stage III, and +50.0% at stage IV). The magnitude of β-oestradiol response was the lowest in
breast stage IV (+15.8%) and the highest at stage V (+41.1%). The progesterone response became significant at stage IV and
testosterone response at stage V. In conclusion, we found that reaching breast stage III was associated with altered responses
of cortisol, insulin and growth hormone concentrations while the responses of the sex hormone concentrations became pronounced
in the last stages of sexual maturation.
Accepted: 17 September 1997 相似文献
909.
Zaichkina SI Klokov DIu Rozanova OM Aptikaeva GF Akhmadieva AKh Smirnova EN 《Genetika》1999,35(9):1274-1279
The dependence between the adaptive response and adaptive dose was studied on the basis of cytogenetic damage in polychromatic erythrocytes of bone marrow cells in mice after a low dose gamma-irradiation in vivo. The adaptive response to doses of 0.1 and 0.2 Gy was found to be retained for at least two months after irradiation. However, the adaptive dose of 0.4 Gy did not induce prolonged adaptive response. 相似文献
910.
Smirnova NI Eroshenko GA Shchelkanova EIu Livanova LF Konnov NP 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1999,(1):3-9
Restriction analysis of temperate cholera phage 139 isolated from Vibrio cholerae P16064, serogroup 0139, showed its DNA to be double-stranded linear with cohesive terminals. DNA-DNA hybridization on nylon membranes revealed that many V. cholerae strains of serogroup 0139 isolated in different regions contained a temperate cholera phage 139 in their genomes. Southern blot hybridization of chromosomal DNA PST-fragments with phage probe showed that the temperate phage 139 was identical to the temperate phage of serogroup II V. eltor. The phage integrated in the chromosome near genes encoding motility (mot) and production of the capsule (cap) and purine (pur). Phage genome is apparently responsible for instability of cap, pur, and mot genes whose products are important for the development of an infectious process in cholera. 相似文献