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M B Smirnova V A Nikulina O L Segal E A Kizim Y S Massino N N Ryazanskaya G I Kolyaskina A D Dmitriev 《Biochemistry. Biokhimii?a》1999,64(6):639-647
Two protocols for sandwich antigen-capture ELISA of human myoglobin were compared. In the first (routine) variant, 14D6 monoclonal antibodies conjugated to horseradish peroxidase were used as the secondary antibodies. Bifunctional antibodies specific for myoglobin/peroxidase were used as the secondary antibodies in the second variant. The myoglobin-binding site of the bifunctional antibodies was similar to that of the 14D6 antibodies, and the second antigen-binding site of the bifunctional antibodies was bound to horseradish peroxidase. When comparing standard calibration curves, the effective concentration of the bifunctional antibodies and that of antibodies conjugated to horseradish peroxidase were made equal. It is shown that the use of bispecific antibodies as the secondary antibodies does not improve the quality of the parameters tested, i.e., the sensitivity of the assay does not increase and the slope of the calibration curve remains constant. 相似文献
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N B Smirnova T V Gorshkova E E Makarovskaia M K Chekhranova S A Afinogenova 《Biokhimii?a (Moscow, Russia)》1987,52(11):1829-1837
A prolonged effect of ACTH on the state of adenylate and guanylate cyclase systems in the adrenal glands of experimental animals was investigated. It was found that in guinea pigs injected with ACTH (4 units daily for 1-50 days) the weight of adrenal glands and the DNA content in these organs increased 2.0-2.5-fold by the end of experiment; the increase in both values was stepwise. The corticosteroid level in the blood varied throughout the experiment: the changes in the DNA content in adrenals and in the corticosteroid content in the blood were oppositely directed. This was accompanied by cyclic changes in the basal and stimulated activities of adenylate and guanylate cyclases and proteinases in the adrenal glands occurring with a periodicity of 6-15 days. The activity peaks for cyclases and protein kinases preceded the rise in the DNA content in the adrenals. A clearcut correlation between the changes in the enzyme activity and the hormone dose was observed. The changes in the basal and stimulated activities of guanylate cyclase seem to be due to the control of cAMP level in the cell (stimulation of cGMP-dependent cAMP phosphodiesterase). Apparently, the periodic changes in the activity of cAMP-dependent protein kinases in the cytoplasmic and nuclear fractions and a relatively high activation of nuclear protein kinases (by 30-60%) in comparison of cytoplasmic ones (8-10%) are related to stimulation of DNA synthesis. It is concluded that the changes in the activity of cyclases and protein kinases play a role in the mechanism of proliferative effect of ACTH. 相似文献
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E P Smirnova N I Ma?sov A M Vikhert 《Biulleten' eksperimental'no? biologii i meditsiny》1986,101(2):156-159
The adequate parameters for simultaneous determination of more than 10 monoamines, their precursors and metabolites (noradrenaline, 3-methoxy-4-hydroxyphenylglycol, 3,4-dihydrooxyphenylglycol++, vanylylmandelic acid, normetanephrine, adrenaline, metanephrine, dopamine, 3-methoxytytramine, 3,4-dihydroxphenylacetic acid, 3,4-dihydroxyphenylalanine, 5-hydroxytryptophane, 5-hydroxyindolacetic acid) by liquid chromatography with electro-chemical detection were suggested for the rat brain and heart. The influence of reserpine, iproniazid, and imipramine on the content of the changes of monoamines and their metabolite levels in the rat brain and heart were also investigated. 相似文献
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A Kh Akhmadieva S I Zaichkina I I Livanova A V Antipov E N Smirnova 《Radiobiologiia》1985,25(3):378-380
The cytochemical study of DNA damage and repair in a Chinese hamster fibroblast culture exposed to gamma-rays and secondary radiation from 70 GeV protons showed no significant differences between the two types of radiation. 相似文献