全文获取类型
收费全文 | 1191篇 |
免费 | 22篇 |
出版年
2022年 | 11篇 |
2021年 | 12篇 |
2020年 | 9篇 |
2019年 | 14篇 |
2018年 | 23篇 |
2017年 | 25篇 |
2016年 | 23篇 |
2015年 | 11篇 |
2014年 | 16篇 |
2013年 | 31篇 |
2012年 | 43篇 |
2011年 | 44篇 |
2010年 | 41篇 |
2009年 | 25篇 |
2008年 | 50篇 |
2007年 | 44篇 |
2006年 | 40篇 |
2005年 | 36篇 |
2004年 | 40篇 |
2003年 | 57篇 |
2002年 | 38篇 |
2001年 | 54篇 |
2000年 | 38篇 |
1999年 | 31篇 |
1998年 | 12篇 |
1992年 | 23篇 |
1991年 | 30篇 |
1990年 | 26篇 |
1989年 | 24篇 |
1988年 | 35篇 |
1987年 | 29篇 |
1986年 | 23篇 |
1985年 | 34篇 |
1984年 | 15篇 |
1983年 | 13篇 |
1982年 | 8篇 |
1981年 | 8篇 |
1978年 | 9篇 |
1977年 | 15篇 |
1976年 | 13篇 |
1975年 | 10篇 |
1974年 | 9篇 |
1973年 | 17篇 |
1972年 | 13篇 |
1971年 | 12篇 |
1970年 | 9篇 |
1969年 | 9篇 |
1968年 | 8篇 |
1967年 | 11篇 |
1966年 | 10篇 |
排序方式: 共有1213条查询结果,搜索用时 31 毫秒
21.
The increase of spontaneous and induced frequencies in mature sperm by N-nitroso-N-ethylurea visible mutation in the stock mei-9(11) of Drosophila melanogaster blocking the excision reparation has been shown. It is suggested that the mutation mei-9(11) blocks the correct way of reparation that is shown in mutator effect of this mutation. 相似文献
22.
At the height of radiation sickness (7-8 days after irradiation) the accretion of proteins of "acute phase" in rat blood was accompanied by diminution of diminution of hydroxylating activity of microsomes determined by the rate of aminopyrine demethylation. There was an invert correlation between these two indices, and the correlation coefficient was -0.9. 相似文献
23.
24.
25.
26.
E A Tolskaya L I Romanova M S Kolesnikova T A Ivannikova E A Smirnova N T Raikhlin V I Agol 《Journal of virology》1995,69(2):1181-1189
27.
The comparative study of E. coli endo-, neuro- and enterotoxins has revealed that there are pronounced differences between them not only in their biological activity tested on the corresponding experimental models, but also in their chemical composition and biochemical characteristics. Electrophoresis in polyacrylamide gel has disclosed the presence of "specific" protein and polysaccharide fractions in these toxins, which can serve as an additional criterion for their differentiation. 相似文献
28.
Chromatin in the nuclei fixed in tissue and in the nuclei isolated by low ionic strength solutions in the presence of Mg2+ is represented by globular (nucleomeric) fibrils, 20-25 nm in diameter. The staphylococcal or endogenous nuclear nuclease splits the chromatin fibrils resulting in fragments corresponding to nucleomers and their multimers. Upon removal of firmly bound Mg2+ the nucleomers unfold to form chains consisting of 4-6-8 nucleosomes. Mild hydrolysis of nuclear chromatin by staphylococcal nuclease results in a split-off of mono-, di- and trimers of nucleomers sedimenting in a sucrose density gradient in the presence of EDTA as particles with the sedimentation coefficients of 37, 47 and 55S, respectively. The sedimentation coefficient for the mononucleomer in a sucrose density gradient with MgCl2 is 45S. Determination of the length of DNA fragments of chromatin split-off by staphylococcal nuclease showed that the nucleomer consists of 8 nucleosomes, while the dimer and trimer of the nucleomer consists of 14-16 and 21-24 nucleosomes, respectively. The nucleomeric monomer undergoes structural transition from the compact (45S) to the "loose" state (37S) after removal of Mg2+. This transition is completely reversible, when the nucleomer contains histone H1. The removal of the latter or dialysis of the nucleomer against EDTA in low ionic strength solutions results in a complete unfolding of the nucleomer into a nucleosomal chain fragment. A model for the nucleomer fibril structure in which the helical organization of the nucleosomal chain in the nucleomer (2 turns with 4 nucleosomes in each) is alternated with the impaired helical bonds between the nucleomers is discussed. The functional significance of the nucleomeric organization of chromatin may be an additional restriction of the site-specific recognition of DNA in chromatin with the possibility of local (at the level of one nucleomer) changes in chromatin conformation excluding this restriction. 相似文献
29.
Three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina. 总被引:2,自引:1,他引:1
下载免费PDF全文
![点击此处可从《Journal of bacteriology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
M B Sherman E V Orlova E A Smirnova S Hovmller N A Zorin 《Journal of bacteriology》1991,173(8):2576-2580
The three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina has been determined at a resolution of 2 nm in the plane and 4 nm in the vertical direction by electron microscopy and computerized image processing on microcrystals of the enzyme. The enzyme forms a large ring-shaped complex containing six each of the large (62-kDa) and small (26-kDa) subunits. The complex is very open, with six well-separated dumbbell-shaped masses surrounding a large cylindrical hole. Each dumbbell is interpreted as consisting of one large and one small subunit. 相似文献
30.
Absolute values of heat capacity for some hydrated globular proteins have been studied by differential scanning calorimetry (DSC) method. It has been found that for the proteins with completely bound water, like in the case of protein solutions, the value of heat capacity of denatured proteins is higher than that prior to denaturation. Depending on temperature and humidity the denatured proteins can be either in high elastic or glass state. Specific heat capacities for these two states have the same values for all proteins and depend only on temperature with a characteristic increment of 0.55 J/g.K. at glass transition. The glass transitions were observed not only in denatured but also in native proteins. As it follows from our results, the main contribution to the heat capacity increment at denaturation is connected with the thermal motion in the protein globule which is in contrast with the commonly accepted ideas. 相似文献