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51.
Caroline Comte Yann Tonin Anne-Marie Heckel-Mager Abdeldjalil Boucheham Alexandre Smirnov Karine Auré Anne Lombès Robert P. Martin Nina Entelis Ivan Tarassov 《Nucleic acids research》2013,41(1):418-433
Mitochondrial mutations, an important cause of incurable human neuromuscular diseases, are mostly heteroplasmic: mutated mitochondrial DNA is present in cells simultaneously with wild-type genomes, the pathogenic threshold being generally >70% of mutant mtDNA. We studied whether heteroplasmy level could be decreased by specifically designed oligoribonucleotides, targeted into mitochondria by the pathway delivering RNA molecules in vivo. Using mitochondrially imported RNAs as vectors, we demonstrated that oligoribonucleotides complementary to mutant mtDNA region can specifically reduce the proportion of mtDNA bearing a large deletion associated with the Kearns Sayre Syndrome in cultured transmitochondrial cybrid cells. These findings may be relevant to developing of a new tool for therapy of mtDNA associated diseases. 相似文献
52.
E. V. Putlyaev A. A. Smirnov E. A. Lazareva G. V. Klink O. V. Karpova J. G. Atabekov 《Moscow University Biological Sciences Bulletin》2013,68(4):169-173
Previously, we obtained a new viral vector based on the Alternanthera mosaic virus strain MU (AltMV-MU). The gene of interest was placed under control of two subgenomic promoters (sgp). Viral vector provided the superexpression of target proteins in Nicotiana benthamiana. In the present work, to increase the level of protein expression, this viral vector was modified and the coat protein gene was placed under control of three sgp. The new viral vector provided superexpression of the protein of interest in plants, and its amount was more than 50% of the total soluble protein in cells. The efficiency of the target protein expression in the plants transformed with viral vectors containing two and three sgp was compared. 相似文献
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54.
The interactions between E- and Z-isomers of 3-O-methoxyimino-pregn-4-ene-20-one and its 17α-hydroxy derivative and transcortin from human blood were investigated. The substitution of the progesterone 3-oxo group for a 3-O-methoxyimino group was shown to diminish the affinity of the steroid for transcortin by approximately one order of magnitude irrespective of the substituent’s orientation. The data suggests that progesterone derivatives substituted thereby must have higher bioavailability compared to progesterone and must not significantly affect the biodynamics of glucocorticoid in vivo. 相似文献
55.
E. V. Borvinskaya I. V. Sukhovskaya A. A. Kochneva O. B. Vasilyeva M. A. Nazarova L. P. Smirnov N. N. Nemova 《Contemporary Problems of Ecology》2016,9(2):195-202
The base levels and seasonal variability of ethoxyresorufin-O-deethylase (EROD) activity, glutathione-S-transferase (GST) activity, reduced glutathione concentration (GSH) and phospholipids concentration (PhL) of Coregonus lavaretus (Linnaeus, 1758) and Coregonus muksun (Pallas, 1814) were investigated. The relationships between this biochemical markers, hepatosomatic index and seasonal growth were also examined. 相似文献
56.
E. M. Vetchinkina V. V. Komakhina D. A. Vysotskii D. V. Zaitsev A. N. Smirnov A. V. Babakov R. A. Komakhin 《Russian Journal of Genetics》2016,52(9):939-951
The chickweed (Stellaria media L.) pro-SmAMP2 gene encodes the hevein-like peptides that have in vitro antimicrobial activity against certain harmful microorganisms. These peptides play an important role in protecting the chickweed plants from infection, and the pro-SmAMP2 gene was previously used to protect transgenic tobacco and Arabidopsis plants from phytopathogens. In this study, the pro-SmAMP2 gene under control of viral CaMV35S promoter or under control of its own pro-SmAMP2 promoter was transformed into cultivated potato plants of two cultivars, differing in the resistance to Alternaria: Yubiley Zhukova (resistant) and Skoroplodny (susceptible). With the help of quantitative real-time PCR, it was demonstrated that transgenic potato plants expressed the pro-SmAMP2 gene under control of both promoters at the level comparable to or exceeding the level of the potato actin gene. Assessment of the immune status of the transformants demonstrated that expression of antimicrobial peptide pro-SmAMP2 gene was able to increase the resistance to a complex of Alternaria sp. and Fusarium sp. phytopathogens only in potato plants of the Yubiley Zhukova cultivar. The possible role of the pro-SmAMP2 products in protecting potatoes from Alternaria sp. and Fusarium sp. is discussed. 相似文献
57.
A. G. Pogorelov A. A. Smirnov V. N. Pogorelova 《Russian Journal of Developmental Biology》2016,47(2):99-102
The given paper summarizes the data on the early mammalian embryo development in culture media containing low oxygen concentration. Experimental results on in vitro modeling the hypoxia for preimplantation development are reviewed. Hypoxic conditions were shown to be available in the female reproductive tract of different mammalian species. The estimation of the embryo developing in vitro exhibits that lower oxygen level in culture media improves embryonic quality. 相似文献
58.
Smirnov V. P. Samokhin A. A. Gavrikov A. V. Kuzmichev S. D. Usmanov R. A. Vorona N. A. 《Plasma Physics Reports》2019,45(5):454-458
Plasma Physics Reports - A new concept of plasma separation of spent nuclear fuel components in a variable-cross-section chamber with a nonuniform magnetic field is proposed. Numerical simulation... 相似文献
59.
Makarov I. O. Klyuev D. A. Smirnov V. F. Smirnova O. N. Anikina N. A. Dikareva N. V. 《Microbiology》2019,88(1):72-78
Microbiology - Effect of low-frequency pulsed magnetic field and of low-intensity laser radiation on mycelial fungi actively degrading various polymer materials was studied. These factors had a... 相似文献
60.