全文获取类型
收费全文 | 10184篇 |
免费 | 542篇 |
国内免费 | 1篇 |
专业分类
10727篇 |
出版年
2023年 | 76篇 |
2022年 | 46篇 |
2021年 | 120篇 |
2020年 | 89篇 |
2019年 | 107篇 |
2018年 | 348篇 |
2017年 | 293篇 |
2016年 | 351篇 |
2015年 | 261篇 |
2014年 | 344篇 |
2013年 | 606篇 |
2012年 | 837篇 |
2011年 | 1042篇 |
2010年 | 585篇 |
2009年 | 275篇 |
2008年 | 575篇 |
2007年 | 520篇 |
2006年 | 492篇 |
2005年 | 362篇 |
2004年 | 286篇 |
2003年 | 285篇 |
2002年 | 218篇 |
2001年 | 131篇 |
2000年 | 219篇 |
1999年 | 164篇 |
1998年 | 114篇 |
1997年 | 72篇 |
1996年 | 74篇 |
1995年 | 87篇 |
1994年 | 67篇 |
1993年 | 52篇 |
1992年 | 70篇 |
1991年 | 61篇 |
1989年 | 61篇 |
1988年 | 42篇 |
1987年 | 63篇 |
1986年 | 43篇 |
1985年 | 70篇 |
1984年 | 65篇 |
1983年 | 57篇 |
1982年 | 59篇 |
1981年 | 65篇 |
1980年 | 62篇 |
1979年 | 65篇 |
1978年 | 53篇 |
1977年 | 58篇 |
1976年 | 44篇 |
1975年 | 45篇 |
1974年 | 48篇 |
1973年 | 46篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
41.
M. Pospíšil 《Folia microbiologica》1967,12(4):367-371
Lymphoid cells prepared from rabbit popliteal lymph nodes were cultivated in medium in which the high molecular weight component was replaced by Carbowax 20 M. This substance, in 0.2% concentration, proved to be a substitute for the high molecular weight component in short-term cultivation, and the viability results were similar to those in medium containing normal rabbit serum or 0.5% human serum albumin. In addition, mitoses and the formation of large basophilic cells resembling the blastoid cells induced during cultivation with phytohaemagglutinin were observed in this medium. The results are discussed. 相似文献
42.
In a model secondary reactionin vitro, a correlation was demonstrated between the size of the antigen dose used for the prestimulation of spleen tissue donors
and the type of antibodies formed in the anamnestic reaction. After a small dose of antigen ΦX 174, the antibody response
three days after prestimulation was of the 19 S (IgM) type, but later secondary contactin vitro (after four months) did not produce a 19 S anamnestic reaction. After large primary doses of antigen, a short interval between
primary and secondary contact led to the formation of 19 and 7 S type antibodies, while after a long interval only 7 S (IgG)
type antibodies were formed. The results are discussed in relation to differences in the size of the antigen dose needed to
induce short-term 19 S and long-term 7 S immunological memory. 相似文献
43.
A?koliv na zá kladě mnoha pokus? se p? edpokládalo, ?e tzv. bÍlkovinná v?etena v buňkách tzn. buně?né inkluse X-viru kaktus? (Ca XV), jsou slo?ena z ?etních prodlou?ených ?ásti Ca XV, p?esto to dosud nebylo proká zá no. Proto jsme se pokusili pomocÍ fluoreskujÍcÍch protilátek doká ?at, ?e bilkovinná v?etena jsou skute?ně agregáty virových ?ástic. V těto práci jsme pouzili tzv. nep?Ímé metody. Nejprve jsme p? sobili na buňky obsahujÍci tato v?etena homologiokým antisé rem proti Ca XV, zÍskanym imunizacÍ králÍk? a teprve potom jsme buňky vlo?ili do roztoku fluoreskujicÍch protilátek proti králicimu γglobulinu. BÍlkovinná v?etena svitila potom ve fluorescen?nÍm mikroskopu silně ?lutozeleně (bylopou?ito fluoresceinisothiocyaná tu). Tato fluorescence ná m uká zala, ?e nastala pozitivnÍ reakce a ?e bÍlkovianá v?etena jsou slo?ena z virových ?ástic. ?etné kontrolnÍ pokusy potvrdily ná? základnÍ pokus. 相似文献
44.
Karel Beneš 《Biologia Plantarum》1962,4(1):61-68
V této práci byly aplikovány na rostlinných objektech reakce s β-oxynaftyl-merkurichloridem a p-nitrobromacetofenonem, popsané k pr?kazu bílkovinných SH na materiálu ?ivo?i?ném. V rostlinných meristematických buňkách obě tyto techniky stejně jako DDD1 a RSR1 jeví vět?inou intensivněj?í zbarvení jader ne? cytoplasmy. Rovně? po aplikaci reakcí na bílkovinné karboxyly a tyrosin je jádro intensivněji zbarveno ne? cytoplasma. Z toho lze soudit, ?e rozdíly v intensitě zbarvení jádra a cytoplasmy v meristematických buňkách rostlinných technikami k pr?kazu bílkovinných SH jsou zp?sobeny spí? rozdílem v celkové koncentraci bílkovin ne? zv??ením podílu cysteinu v jaderných bílkovinách. 相似文献
45.
International Journal of Biometeorology - 相似文献
46.
47.
Adolf Češka 《Folia Geobotanica》1966,1(3):93-100
Various possibilities of usingSörensen's coefficient of floristic similarity in plant sociology are summarized. A new formula (11), derived from that bySörensen and based on constancy values, is suggested in order to calculate the mean floristic similarity within a set of relevés. 相似文献
48.
Adolf Češka 《Folia Geobotanica》1966,1(2):93-100
Various possibilities of usingSörensen’s coefficient of floristic similarity in plant sociology are summarized. A new formula (11). derived from that bySörensen and based on constancy values, is suggested in order to calculate the mean floristic similarity within a set of relevés. 相似文献
49.
Anna Kocková-Kratochvílová Anna Vojtková-Lepšíková J. šandula Mária Pokorná 《Folia microbiologica》1966,11(3):200-209
The authors submit a taxonomic evaluation of an intermediate group of strains between the speciesSaccharomyces carlsbergensis Hansen andSaccharomyces cerevisiae Hansen. The material consisted of atypical strains of “bottom” brewer’s yeasts and the synonymous strainsSaccharomyces monacensis Hansen andSaccharomyces mandshuricus Saito. It was found that there were two different serological types in the speciesSaccharomyces carlsbergensis, one of which was characterized by the presence of antigen “C” and was typical for this species, while the other possessed antigen “M” and was grouped roundSaccharomyces monacensis. This second serological type merges with a group of strains which gives only one third fermentation of raffinose, so that it is actually an intermediate betweenSaccharomyces cerevisiae Hansen andSaccharomyces carlsbergensis Hansen and indicates the course of progressive development from the former species to the latter. No close similarity was found betweenSaccharomyces mandshuricus Saito and some of the strains of the transitional group or typical representatives of the two main species, and the authors therefore consider that there is some obscurity as to its synonymity withSaccharomyces carlsbergensis. 相似文献
50.
Antiserum был подготовлен противингибитор вируса haemagglutination (IVH)одна из эмбриона chorioallantoic мембр анныепутем иммун изации морских св инокЭто анти-инги битора в сыворотке заблокирован ингибирование IVH из chorioallantoicмембран в haemagglutination испытанияно ника кого влияния на ре цепторы эритроци товСыворотке кро] ви не влияет рост chorioallantoic мембраны клет ок инет нейтрализ овать воздействи е на вирус гриппа Она снизилась спо собность chorioallantoic мембр ан для adsorb вируса гри ппа, а также степен ь размножения вир усав такого рода тканиАвторы хоте ли бы поблагодари ть г-н Г. Ruttkay-Ne -палубе и для проведения эле ктрофореза меру ния. 相似文献