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81.
Rodrigo Arturo Rivera-Solís Santy Peraza-Echeverria Ileana Echevarría-Machado Virginia Aurora Herrera-Valencia 《Annals of microbiology》2014,64(2):551-559
Purple acid phosphatases (PAPs) are metallophosphoesterase enzymes involved in the acquisition and recycling of phosphorus. PAP phytases from microorganisms and plants are responsible for the dephosphorylation of phytate. Phytate is the main storage form of phosphorus in plant seeds and constitutes the major form of organic phosphorus present in soil. Although some phosphatases have been studied in Chlamydomonas reinhardtii, no gene coding for PAPs have so far been characterized. In this study, six PAP homologue genes were identified and characterized in silico in C. reinhardtii (CrPAP1 to CrPAP6). A metallophosphoesterase domain including the seven conserved residues characteristic of PAP enzymes was found in all six CrPAPs. The phylogenetic tree comprising PAP homologue sequences from microalgae, plants, and animals showed nine major clades and CrPAPs resolved in four of them. A constitutive expression was found for CrPAP2, CrPAP3, CrPAP4, and CrPAP6 in all media tested, while CrPAP1 and CrPAP5 were induced by the addition of phytate in a medium without phosphate salts. Our results provide a starting point for further functional analysis of the CrPAP gene family, and the evaluation of their potential as phytases in C. reinhardtii. 相似文献
82.
Estefany Goncalves Ileana Herrera Milén Duarte Ramiro O. Bustamante Margarita Lampo Grisel Velásquez Gyan P. Sharma Shaenandhoa García-Rangel 《PloS one》2014,9(10)
Lantana camara, a native plant from tropical America, is considered one of the most harmful invasive species worldwide. Several studies have identified potentially invasible areas under scenarios of global change, on the assumption that niche is conserved during the invasion process. Recent studies, however, suggest that many invasive plants do not conserve their niches. Using Principal Components Analyses (PCA), we tested the hypothesis of niche conservatism for L. camara by comparing its native niche in South America with its expressed niche in Africa, Australia and India. Using MaxEnt, the estimated niche for the native region was projected onto each invaded region to generate potential distributions there. Our results demonstrate that while L. camara occupied subsets of its original native niche in Africa and Australia, in India its niche shifted significantly. There, 34% of the occurrences were detected in warmer habitats nonexistent in its native range. The estimated niche for India was also projected onto Africa and Australia to identify other vulnerable areas predicted from the observed niche shift detected in India. As a result, new potentially invasible areas were identified in central Africa and southern Australia. Our findings do not support the hypothesis of niche conservatism for the invasion of L. camara. The mechanisms that allow this species to expand its niche need to be investigated in order to improve our capacity to predict long-term geographic changes in the face of global climatic changes. 相似文献
83.
Georges Seingier Ileana Espejel José Luis Fermán-Almada Oscar Delgado González Gabriela Montaño-Moctezuma Isaac Azuz-Adeath Guillermo Aramburo-Vizcarra 《Ecological Indicators》2011,11(2):633-642
To better understand the relation between coastal areas and their influence on social and economic conditions of municipalities, a set of indexes were developed to characterize coastal Mexico with an interdisciplinary approach. The composite of these bio-physical, social and economic indexes is referred to as an “integrated coastal orientation index”. Coastal characteristics should be taken into account in the coastal management policy formulation of a given region, and this index can be used to identify key areas for environmental protection or economic development at a national level. The results suggest that municipalities must manage their coastal jurisdictions, a large and diverse array of coastal space and ecosystems, medium to weak spatial demographics and settlement structures, infrastructure to improve quality of life, an overall low coastal population income, and various coastal activities. Spatial heterogeneity along the littoral zone in the coastal orientation led us to conduct a state analysis. We discuss these values and identify five highly coastal-orientated states in the extremes of the country. The development of this tool could help to pin-point different types of key areas within the regions studied and provide a tool to guide regional priorities. As an example, using a combination of extreme values of bio-physical, social and economic indexes was used to identify specific municipalities as candidates for environmental protection and others as areas where development can continue. Choices of indexes are discussed, and the relative importance given to each component of the coastal orientation (rather bio-physical, social and/or economic) will define the course of the coast's development and its sustainability. 相似文献
84.
Haobo Zhang Perminder S. Sachdev Wei Wen Nicole A. Kochan John D. Crawford Henry Brodaty Melissa J. Slavin Simone Reppermund Kristan Kang Julian N. Trollor 《PloS one》2013,8(11)
Language has been extensively investigated by functional neuroimaging studies. However, only a limited number of structural neuroimaging studies have examined the relationship between language performance and brain structure in healthy adults, and the number is even less in older adults. The present study sought to investigate correlations between grey matter volumes and three standardized language tests in late life. The participants were 344 non-demented, community-dwelling adults aged 70-90 years, who were drawn from the population-based Sydney Memory and Ageing Study. The three language tests included the Controlled Oral Word Association Task (COWAT), Category Fluency (CF), and Boston Naming Test (BNT). Correlation analyses between voxel-wise GM volumes and language tests showed distinctive GM correlation patterns for each language test. The GM correlates were located in the right frontal and left temporal lobes for COWAT, in the left frontal and temporal lobes for CF, and in bilateral temporal lobes for BNT. Our findings largely corresponded to the neural substrates of language tasks revealed in fMRI studies, and we also observed a less hemispheric asymmetry in the GM correlates of the language tests. Furthermore, we divided the participants into two age groups (70-79 and 80-90 years old), and then examined the correlations between structural laterality indices and language performance for each group. A trend toward significant difference in the correlations was found between the two age groups, with stronger correlations in the group of 70-79 years old than those in the group of 80-90 years old. This difference might suggest a further decline of language lateralization in different stages of late life. 相似文献
85.
Reactions of cis-diamminedichloroplatinum(II) with phosphonoformic acid (PFA), phosphonoacetic acid (PAA), and methylenediphosphonic acid (MDP) yield various phosphonatoplatinum(II) chelates which were characterized by phosphorus-31 NMR spectroscopy. The P-31 resonances for the chelates appear at 6–12 ppm downfield as compared to the uncomplexed ligands. All complexes exhibit monoprotic acidic behavior in the pH range 2–10. The chemical shift-pH profiles yielded acidity constants, 1.0 × 10−4, 1.5 × 10−4, and 1.3 × 10−6 M−1, for the PFA, PAA, and MDP chelates. In addition to the monomeric chelate, MDP formed a bridged diplatinum(II,II) complex when it reacted with cis-Pt (NH3)2(H2O)22+. The P-31 resonance for this binuclear complex appears at 22 ppm downfield from the unreacted ligand.
Rate data for the complexation reactions of the phosphonate ligands with the dichloroplatinum complex are consistent with a mechanism in which a monodentate complex is formed initially through rate-limiting aquation process of the platinum complex, followed by a rapid chelation. For the PFA and PAA complexes, initial binding sites are the carboxylato oxygens. Implications of the various binding modes of the phosphonates in relationship to their antiviral activities are discussed. 相似文献
86.
The expression of MMP‐1 and MMP‐9 is up‐regulated by smooth muscle cells after their cross‐talk with macrophages in high glucose conditions
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Razvan Daniel Macarie Mihaela Vadana Letitia Ciortan Monica M. Tucureanu Andrea Ciobanu Dragos Vinereanu Ileana Manduteanu Maya Simionescu Elena Butoi 《Journal of cellular and molecular medicine》2018,22(9):4366-4376
Patients with diabetes mellitus have an increased risk of myocardial infarction and coronary artery disease‐related death, exhibiting highly vulnerable plaques. Many studies have highlighted the major role of macrophages (MAC) and smooth muscle cells (SMC) and the essential part of metalloproteases (MMPs) in atherosclerotic plaque vulnerability. We hypothesize that in diabetes, the interplay between MAC and SMC in high glucose conditions may modify the expression of MMPs involved in plaque vulnerability. The SMC‐MAC cross‐talk was achieved using trans‐well chambers, where human SMC were grown at the bottom and human MAC in the upper chamber in normal (NG) or high (HG) glucose concentration. After cross‐talk, the conditioned media and cells were isolated and investigated for the expression of MMPs, MCP‐1 and signalling molecules. We found that upon cross‐talk with MAC in HG, SMC exhibit: (i) augmented expression of MMP‐1 and MMP‐9; (ii) significant increase in the enzymatic activity of MMP‐9; (iii) higher levels of soluble MCP‐1 chemokine which is functionally active and involved in MMPs up‐regulation; (iv) activated PKCα signalling pathway which, together with NF‐kB are responsible for MMP‐1 and MMP‐9 up‐regulation, and (v) impaired function of collagen assembly. Taken together, our data indicate that MCP‐1 released by cell cross‐talk in diabetic conditions binds to CCR2 and triggers MMP‐1 and MMP‐9 over‐expression and activity, features that could explain the high vulnerability of atherosclerotic plaque found at diabetic patients. 相似文献
87.
Franca Serafini-Cessi Nadia Malagolini Stefania Guerrini Ileana Turrini 《Glycoconjugate journal》1995,12(6):773-779
We have previously shown that human colon carcinoma CaCo-2 cells express the Sda-1,4-N-acetylgalactosaminyltransferase (Sda-GalNAc-transferase) and that the enzyme activity correlates with the degree of enterocytic differentiation. Here we report that a large amount of this glycosyltransferase is released in soluble form, particularly when CaCo-2 cells are maintained in culture for more than 3 weeks in order to ensure an higher degree of enterocyte differentiation. The soluble enzyme was concentrated and partially purified by Blue-Sepharose and fetuin-Sepharose chromatography. The substrate specificity of the partially purified enzyme was similar to that of Sda-enzyme from epithelial cells of colon mucosa, and for its activity strictly required the presence in acceptors of NeuAc in 2,3-linkage to subterminal galactose. Among the low molecular glycans tested, NeuAc2,3Gal1,4GlcNAc appeared to be the best acceptor, whereas sialyl-Lewisx and sialyl-Lewisa did not serve as acceptors, indicating that the fucosylation of sub-terminal GlcNAc hindered the transferase activity. Contrary to this, the activity towards a disialylated acceptor such as di-sialyl-lacto-N-tetraose was reduced but not abolished. When CaCo-2 cells were cultured on porous membranes and the transferase activity assayed in medium collected from chambers corresponding to either the apical or basolateral face of highly differentiated CaCo-2 cells, a preferential release from the basolateral surface was found. Considering that Sda-GalNAc-transferase is mainly located in the large intestine, current results support the notion that colonic cells largely contribute to the presence of the enzyme in human plasma. 相似文献
88.
Human T cells recovered from human/Balb radiation chimeras are hypersensitive to human immunodeficiency virus type 1 infection.
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O Shapira-Nahor H Marcus H Segall I Lubin S Slavin A Panet Y Reisner 《Journal of virology》1997,71(6):4495-4501
Replication of human immunodeficiency virus type 1 (HIV-1) is regulated by virus-encoded regulatory proteins, as well as by a variety of cellular factors. Productive infection of human T lymphocytes by HIV-1 is dependent upon the activation status of the target cells. In general, short-term mitogenic stimulation of CD4 T cells is used to enhance infection of peripheral blood mononuclear cells (PBMC) in vitro. Recently, we demonstrated that adoptive transfer of human PBMC into lethally irradiated BALB/c mice, radioprotected with severe combined immunodeficiency (SCID) mouse bone marrow, leads to marked T-cell activation and proliferation. In the present study, we investigated the effect of such xenoactivation of human T cells on their susceptibility to HIV-1 infection. Human cells that were recovered from human/Balb radiation chimeras supported efficient replication of laboratory strains of HIV-1, as well as of HIV-1 clinical isolates. The multiplicity of infection required to attain effective virus replication in the recovered xenoactivated human cells was 10- to 100-fold lower than that needed for infection of short- or long-term phytohemagglutinin (PHA)-stimulated blasts or of various T-cell lines. Analysis of human cell surface activation markers has indicated that xenoactivation in the mouse, in contrast to in vitro stimulation with PHA, is associated with a marked downregulation of CD25 (interleukin 2 receptor). Our results demonstrate that human cells recovered from human/Balb radiation chimeras, which are hypersensitive to HIV-1 infection, differ from in vitro-stimulated cells in their activation status. Therefore, this system could be used to study host factors that participate in HIV-1 infection and replication in vitro and in vivo. 相似文献
89.
Detection of guanylate cyclases A and B stimulated by natriuretic peptides in gastrointestinal tract of rat 总被引:6,自引:0,他引:6
Maria Grazia Rambotti Ileana Giambanco Antonio Spreca 《Journal of molecular histology》1997,29(2):117-126
Summary The ultracytochemical localization of membrane-bound guanylate cyclases A and B has been studied after stimulation with atrial
natriuretic peptide, C-type natriuretic peptide and brain natriuretic peptide in the gastrointestinal tract of rat. The two
isoforms are stimulated differently by the three peptides. The results showed that the atrial and C-type natriuretic peptides
stimulated guanylate cyclase activity, whereas the brain peptide seemed not to activate enough of the enzyme to detect. The
guanylate cyclase activity had a wider distribution in stomach and small intestine than in large intestine; nevertheless,
the reaction product of guanylate cyclase A activity had a wider localization in the stomach, whereas the reaction product
of guanylate cyclase B activity had a wider distribution in the small intestine. In the small and large intestine, we detected
mostly similar localizations of guanylate cyclase activity irrespective of the peptide used; in the stomach the reaction products
of guanylate cyclase A and B were detected in different cell types or in different sites of the same cell. In all the gastrointestinal
tract, guanylate cyclase activity was detected mainly in three types of cells: exocrine and endocrine cells; undifferentiated
and mature epithelial cells; and smooth muscle cells. These localizations of guanylate cyclase activity suggest its role in
regulating glandular secretion, cellular proliferation and muscular activity.
This revised version was published online in November 2006 with corrections to the Cover Date. 相似文献
90.
Sara Faranda Annalisa Frattini Ileana Zucchi Cristina Patrosso Luciano Milanesi Cristina Montagna Paolo Vezzoni 《Genomics》1996,34(3):323
Two new genes were identified and mapped by searching the EST databases with genomic sequences obtained from putative CpG islands of the rodent–human hybrid X3000. Previous mapping of these CpG islands in the proximity of the host cell factor (HCFC1) and GdX genes automatically localized these two new genes to Xq28 in the interval between the L1 cell adhesion molecule (L1CAM) and the glucose-6-phosphate dehydrogenase (G6PD) loci. Both genes are relatively short, contain an ORF of 261 and 105 amino acids, respectively, and are ubiquitously expressed. Combining sequencing of selected CpG islands, derived from hybrids containing small portions of the human genome, with an EST database search is an easy method of identifying and mapping new genes to specific regions of the genome. 相似文献