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排序方式: 共有267条查询结果,搜索用时 15 毫秒
191.
Morten J. Bjerrum Danilo R. Casimiro I. -Jy. Chang Angel J. Di Bilio Harry B. Gray Michael G. Hill Ralf Langen Gary A. Mines Lars K. Skov Jay R. Winkler Deborah S. Wuttke 《Journal of bioenergetics and biomembranes》1995,27(3):295-302
Photochemical techniques have been used to measure the kinetics of intramolecular electron transfer in Ru(bpy)2(im)(His)2+-modified (bpy = 2,2-bipyridine; im = imidazole) cytochromec and azurin. A driving-force study with the His33 derivatives of cytochromec indicates that the reorganization energy () for Fe2+Ru3+ ET reactions is 0.8 eV. Reductions of the ferriheme by either an excited complex,*Ru2+, or a reduced complex, Ru+, are anomalously fast and may involve formation of an electronically excited ferroheme. The distance dependence of Fe2+Ru3+ and Cu+Ru3+ electron transfer in 12 different Ru-modified cytochromes and azurins has been analyzed using a tunneling-pathway model. The ET rates in 10 of the 12 systems exhibit an exponential dependence on metal-metal separation (decay constant of 1.06 å–1) that is consistent with predictions of the pathway model. 相似文献
192.
193.
The neotropical palm genus Hyospathe includes two species, H. elegans and H. macrorhachis. Based on morphological, anatomical and palynological evidence, most of the names previously published in the genus have been synonymized into H. elegans. A detailed treatment of the variation and distribution of this broadly defined and widespread species is given. 相似文献
194.
Potential impacts of climate change on the distributions and diversity patterns of European mammals 总被引:2,自引:1,他引:1
Irina Levinsky Flemming Skov Jens-Christian Svenning Carsten Rahbek 《Biodiversity and Conservation》2007,16(13):3803-3816
The Intergovernmental Panel on Climate Change (IPCC) predicts an increase in global temperatures of between 1.4°C and 5.8°C
during the 21st century, as a result of elevated CO2 levels. Using bioclimatic envelope models, we evaluate the potential impact of climate change on the distributions and species
richness of 120 native terrestrial non-volant European mammals under two of IPCC’s future climatic scenarios. Assuming unlimited
and no migration, respectively, our model predicts that 1% or 5–9% of European mammals risk extinction, while 32–46% or 70–78%
may be severely threatened (lose > 30% of their current distribution) under the two scenarios. Under the no migration assumption
endemic species were predicted to be strongly negatively affected by future climatic changes, while widely distributed species
would be more mildly affected. Finally, potential mammalian species richness is predicted to become dramatically reduced in
the Mediterranean region but increase towards the northeast and for higher elevations. Bioclimatic envelope models do not
account for non-climatic factors such as land-use, biotic interactions, human interference, dispersal or history, and our
results should therefore be seen as first approximations of the potential magnitude of future climatic changes. 相似文献
195.
Basaiawmoit RV Oliveira CL Runager K Sørensen CS Behrens MA Jonsson BH Kristensen T Klintworth GK Enghild JJ Pedersen JS Otzen DE 《Journal of molecular biology》2011,408(3):503-513
Human transforming growth factor β induced protein (TGFBIp) is composed of 683 residues, including an N-terminal cysteine-rich (EMI) domain, four homologous fasciclin domains, and an Arg-Gly-Asp (RGD) motif near the C-terminus. The protein is of interest because mutations in the TGFBI gene encoding TGFBIp lead to corneal dystrophy (CD), a condition where protein aggregates within the cornea compromise transparency. The complete three-dimensional structure of TGFBIp is not yet available, with the exception of a partial X-ray structure of the archetype FAS1 domain derived from Drosophila fasciclin-1. In this study, small-angle X-ray scattering (SAXS) models of intact wild-type (WT) human TGFBIp and a mutant (R124H) are presented. The mutation R124H leads to a variant of granular CD. The deduced structure of the TGFBIp monomer consists of four FAS1 domains in a simple “beads-on-a-string” arrangement, constructed by the superimposition of four consecutive Drosophila fasciclin domains. The SAXS-based model of the TGFBIp R124H mutant displayed no structural differences from WT. Both WT TGFBIp and the R124H mutant formed trimers at higher protein concentrations. The similar association properties and three-dimensional shape of the two proteins suggest that the mutation does not induce any major structural rearrangements, but points towards the role of other corneal-specific factors in the formation of corneal R124H deposits. 相似文献
196.
C cile Albenne A. Bart Van der Veen Gabrielle Potocki-v ron se Gilles Joucla Lars Skov Osman Mirza Michael Gajhede Pierre Monsan Magali Remaud-simeon 《Biocatalysis and Biotransformation》2003,21(4):271-276
Rational engineering of amylosucrase required detailed investigations of the molecular basis of catalysis. Biochemical characterization of the enzyme coupled to structural analyses enabled the polymerization mechanism to be elucidated. This provided key information for successfully changing amylosucrase to a hydrolase or an improved polymerase using site-directed mutagenesis. In parallel, a combinatorial approach was developed to further improve the catalyst. The method, based on random mutagenesis and recombination of parent sequences, has generated libraries of variants. These were searched for improved polymer formation using a first step of selection followed by a screening test including a double detection method. Several mutants with desired properties have been isolated, their sequences revealed that they could not have been designed following a rational approach. 相似文献
197.
Survival of 0+ year pike Esox lucius (20–31 mm L T ) was evaluated over the first week following stocking in ponds (16 m2 ) depending on water transparencies (low and high), habitat complexity (low and high) and food availability (zooplankton). A multiple regression analysis showed that survival, essentially as a result of cannibalism, decreased with increasing size heterogeneity of pike, and the decrease was steeper when no food was available. Survival was equal in clear water and water containing copper chlorophyllin (chlorophyll), and slightly higher in complex than in simple habitats, but only when alternative prey availability was low. Conversely, growth in water containing chlorophyll was slightly faster than in clear water. This suggests that initial size heterogeneity is the prevailing factor controlling mortality when stocking 0+ year pike almost irrespective of the stocking environment. 相似文献
198.
The aggregation behavior of zinc-free insulin has been studied by small-angle neutron scattering as a function of pH and ionic strength of the solution. The pair distance distribution functions for the 12 samples have been obtained by indirect Fourier transformation. The results show that the diameter of the aggregates is 40 Å at pH 11 and 10 mM NaCl, independent of the protein concentration. The largest diameter of about 120 Å is found for pH 8, 100 mM NaCl, and a protein concentration of 10 mg/ml. Estimates of the pair distance distribution functions, free of inter-particle correlation effects, were obtained by an indirect Fourier transformation, omitting the data at small scattering vectors, which are influenced by these effects. By this procedure the weight-averaged molecular mass and the average radius of gyration were determined. These parameters vary from 1.3 times the monomer mass and 14 Å, to 6.8 times the monomer mass and 31 Å, respectively. The mass distribution between the oligomers was determined by a model based on the crystal structure of zinc-free insulin. The results from this model and the Fourier transformations have been compared to an equilibrium model recently introduced by Kadima et al. (1993). The neutron scattering results agree well with the predictions of this model except that broader mass distributions are suggested by neutron scattering.
Correspondence to: J. Skov Pedersen 相似文献
199.
Marianne Sunde Gunnar Skov Simonsen Jannice Schau Sletteme?s Inger B?ckerman Madelaine Norstr?m 《PloS one》2015,10(6)
Antimicrobial resistant Escherichia coli (n=331) isolates from humans with bloodstream infections were investigated for the presence of class 1 and class 2 integrons. The integron cassettes arrays were characterized and the findings were compared with data from similar investigations on resistant E. coli from meat and meat products (n=241) produced during the same time period. All isolates were obtained from the Norwegian monitoring programs for antimicrobial resistance in human pathogens and in the veterinary sector. Methods used included PCR, sequencing, conjugation experiments, plasmid replicon typing and subtyping, pulsed-field-gel-electrophoresis and serotyping. Integrons of class 1 and 2 occurred significantly more frequently among human isolates; 45.4% (95% CI: 39.9-50.9) than among isolates from meat; 18% (95% CI: 13.2 -23.3), (p<0.01, Chi-square test). Identical cassette arrays including dfrA1-aadA1, aadA1, dfrA12-orfF-aadA2, oxa-30-aadA1 (class 1 integrons) and dfrA1-sat1-aadA1 (class 2 integrons) were detected from both humans and meat. However, the most prevalent cassette array in human isolates, dfrA17-aadA5, did not occur in isolates from meat, suggesting a possible linkage between this class 1 integron and a subpopulation of E. coli adapted to a human host. The drfA1-aadA1 and aadA1 class 1 integrons were found frequently in both human and meat isolates. These isolates were subjected to further studies to investigate similarities with regard to transferability, plasmid and host strain characteristics. We detected incF plasmids with pMLST profile F24:A-:B1 carrying drfA1-aadA1 integrons in isolates from pork and in a more distantly related E. coli strain from a human with septicaemia. Furthermore, we showed that most of the class 1 integrons with aadA1 were located on incF plasmids with pMLST profile F51:A-:B10 in human isolates. The plasmid was present in unrelated as well as closely related host strains, demonstrating that dissemination of this integron also could be attributed to clonal spread. In conclusion, among the systematically collected isolates from two different sources, some significant differences concerning integron prevalence and integron variants were observed. However, closely related plasmids as vehicles for specific class 1 integrons in isolates from meat and from a human with bloodstream infection were found. The occurrence of similar multi-resistance plasmids in bacteria from a food source and from a human clinical sample highlights the possible role of meat as a source of resistance elements for pathogenic bacteria. 相似文献
200.
A novel method for the initiation of intramolecular electron transfer reactions in azurin is reported. The method is based on laser photoexcitation of covalently attached thiouredopyrenetrisulfonate (TUPS), the reaction that generates the low potential triplet state of the dye with high quantum efficiency. TUPS derivatives of azurin, singly labeled at specific lysine residues, were prepared and purified to homogeneity by ion exchange HPLC. Transient absorption spectroscopy was used to directly monitor the rates of the electron transfer reaction from the photoexcited triplet state of TUPS to Cu(II) and the back reaction from Cu(I) to the oxidized dye. For all singly labeled derivatives, the rate constants of copper ion reduction were one or two orders of magnitude larger than for its reoxidation, consistent with the larger thermodynamic driving force for the former process. Using 3-D coordinates of the crystal structure of Pseudomonas aeruginosa azurin and molecular structure calculation of the TUPS modified proteins, electron transfer pathways were calculated. Analysis of the results revealed a good correlation between separation distance from donor to Cu ligating atom (His-N or Cys-S) and the observed rate constants of Cu(II) reduction. 相似文献