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Virus Replication in Enucleate Cells: Vesicular Stomatitis Virus and Influenza Virus 总被引:28,自引:17,他引:11 下载免费PDF全文
The requirement of the presence of a nucleus for the replication of vesicular stomatitis virus and influenza virus has been examined by following the growth and development of these viruses in enucleate BS-C-1 cells. Vesicular stomatitis virus replicates normally in enucleate cells with the rate of production of infectious virus, the amount of virus-specific protein synthesis, and the type of proteins produced being essentially the same in nucleate and enucleate cells. Influenza virus does not replicate in enucleate cells, no virus gene products can be detected, and there is no inhibition of cellular protein synthesis. 相似文献
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Subcellular localization of DNA-binding protein BA by immunofluorescence and immunoelectron microscopy 总被引:3,自引:2,他引:1 下载免费PDF全文
Nonhistone protein BA has been shown to decrease in amount in the chromatin of growth- stimulated normal rat liver (Yeoman et al. 1975. Cancer Res. 35:1249-1255) and in mitogen-stimulated normal human lymphocytes (Yeoman et al. 1976. Exp. Cell Res. 100:47- 55.). Subsequently, protein BA was purified and was shown to prefer to bind to double- stranded A-T-rich DNAs (Catino et al. 1978. Biochemistry. 17:983-987.). Immunization of rabbits with highly purified protein BA has resulted in the production of a specific antibody. A specific immunoreactivity for chromosomal protein BA has been demonstrated by immunoelectrophoresis and double antibody immunoprecipitation analysis with rabbit anti-BA immunoglobulin and IgG fractions. Light microscope examination of normal rat liver crysections by the indirect immunofluorescence procedure has demonstrated a cytoplasmic as well as a nuclear localization for protein BA with a pronounced perinucleolar fluorescence. Immunoelectron microscopy employing the peroxidase antiperoxidase method of antigen localization has confirmed the immunofluorescence data and has show a heterochromatin localization for protein BA. The relationship of the localization of protein BA to gene control in quiescent cells or to configurations of heterochromatin as well as the marked reduction in the amounts of protein BA which occur in stimulated growth states remains to be defined. 相似文献
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Baković Jovana Yu Bess Yi Kun Silva Daniel Chew Sew Peak Kim Sangeun Ahn Sun-Hee Palmer Laura Aloum Lujain Stanzani Giacomo Malanchuk Oksana Duchen Michael R. Singer Mervyn Filonenko Valeriy Lee Tae-Hoon Skehel Mark Gout Ivan 《Molecular and cellular biochemistry》2019,455(1-2):91-97
Molecular and Cellular Biochemistry - Cisplatin has been extensively used as a chemotherapeutic agent since around 40 years, though its usage is limited due to severe adverse effects like... 相似文献