Seed preference and energy intake of Goldfinches Carduelis carduelis in the breeding season

The food of a population of Europeans Goldfinches breeding in Orchards was studied. More than 5% of the total foodstuff consumed was met by the seeds of seven food plants. Water, lipid, carbohydrate and protein content was determined from 20 different plants which Goldfinches used during the breeding season in order to calculate energy content. The energy of fresh seeds varied from 1.88 kJ/g in Tussilago farfara to 12.14 kJ/g in Dactylis glomerata. The dependence of the rate of energy intake (profitability) on the pecking rate and the size (weight) of the seeds was calculated. The profitability of various seeds is in agreement with the relative number of birds using them. Calculation of the feeding time that a Goldfinch needs during daylight hours, if it feeds only on a particular plant, indicates that there are several foods that cannot fill the energy demands of the bird during the breeding season. These are eaten only if others with a high energy content are not available. Thus food selection in Goldfinches depends, apart from availability, on seed profitability. The oxygen consumption of a male Goldfinch was significantly higher when the animal was feeding on seed heads of Tussilago farfara and Taraxacum officinale than when it fed on loose dry seeds, and so, different feeding techniques have to be taken into account in calculating net energy gain.     

Chicken major histocompatibility complex congenic lines differ in the percentages of lymphocytes bearing CD4 and CD8 antigens

In the experiments to be described two congenic inbred lines CB and CC and two recombinant lines CB.R1 and CC.R1 were used. All four lines differ only in regard to the major histocompatibility complex (MHC). To determine the percentage distributions of the two cell subsets in peripheral blood lymphocytes (PBL) in these lines, monoclonal antibodies to these two antigens were used. By FACScan there were more CD4+PBL in CB and CB.R1 lines (share B-F/B-L region, controlling class I/class II antigens with line CB) than CC and CC.R1, while the reverse was true with CD8+ subsets. There were more CD8+ PBL in the CC and CC.R1 lines and less in CB and CB.R1 lines. The ratio of CD4+ to CD8+ in CB chickens was 3.4 +/- 0.2 and in CC chickens 1.6 +/- 0.1.     

Microradioisotopic assay for indoleamine N-methyltransferase and analysis of products by liquid chromatography

A rapid method for the separation of tryptamine, 5-hydroxytryptamine, and their N-methylated derivatives is described. The method involves liquid chromatography using a cation exchange column with the eluant monitored either by ultraviolet or fluorescence spectroscopy. The latter technique permits the detection of picogram quantities of indoleamines. Using normal-phase liquid chromatography a complete separation of tryptamine, its N-methylated derivatives, and their β-carboline analogs was also achieved. A radioisotopic assay with the potential to detect indoleamine N-methyltransferase activity in milligram quantities of rabbit lung tissue was developed. The radioisotopically labeled products formed from a number of substrates in such assays were characterized by liquid chromatography.