排序方式: 共有94条查询结果,搜索用时 625 毫秒
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Bodo Brand Frieder Hadlich Bettina Brandt Nicolas Schauer Katharina L. Graunke Jan Langbein Dirk Repsilber Siriluk Ponsuksili Manfred Schwerin 《PloS one》2015,10(4)
In the past decade the number of studies investigating temperament in farm animals has increased greatly because temperament has been shown not only to affect handling but also reproduction, health and economically important production traits. However, molecular pathways underlying temperament and molecular pathways linking temperament to production traits, health and reproduction have yet to be studied in full detail. Here we report the results of metabolite profiling of the prefrontal cortex and serum of cattle with distinct temperament types that were performed to further explore their molecular divergence in the response to the slaughter procedure and to identify new targets for further research of cattle temperament. By performing an untargeted comprehensive metabolite profiling, 627 and 1097 metabolite features comprising 235 and 328 metabolites could be detected in the prefrontal cortex and serum, respectively. In total, 54 prefrontal cortex and 51 serum metabolite features were indicated to have a high relevance in the classification of temperament types by a sparse partial least square discriminant analysis. A clear discrimination between fearful/neophobic-alert, interested-stressed, subdued/uninterested-calm and outgoing/neophilic-alert temperament types could be observed based on the abundance of the identified relevant prefrontal cortex and serum metabolites. Metabolites with high relevance in the classification of temperament types revealed that the main differences between temperament types in the response to the slaughter procedure were related to the abundance of glycerophospholipids, fatty acyls and sterol lipids. Differences in the abundance of metabolites related to C21 steroid metabolism and oxidative stress indicated that the differences in the metabolite profiles of the four extreme temperament types could be the result of a temperament type specific regulation of molecular pathways that are known to be involved in the stress and fear response. 相似文献
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QTL for the heritable inverted teat defect in pigs 总被引:2,自引:0,他引:2
Elisabeth Jonas Heinz-Josef Schreinemachers Tina Kleinwächter Cemal Ün Ina Oltmanns Sylvio Tetzlaff Danyel Jennen Dawid Tesfaye Siriluck Ponsuksili Eduard Murani Heinz Juengst Ernst Tholen Karl Schellander Klaus Wimmers 《Mammalian genome》2008,19(2):127-138
The mothering ability of a sow largely depends on the shape and function of the mammary gland. The aim of this study was to
identify QTL for the heritable inverted teat defect, a condition characterized by disturbed development of functional teats.
A QTL analysis was conducted in a porcine experimental population based on Duroc and Berlin Miniature pigs (DUMI). The significant
QTL were confirmed by linkage analysis in commercial pigs according to the affected sib pair design and refined by family-based
association test (FBAT). Nonparametric linkage (NPL) analysis revealed five significant and seven suggestive QTL for the inverted
teat defect in the porcine experimental population. In commercial dam lines five significant NPL values were detected. QTL
regions in overlapping marker intervals or close proximity in both populations were found on SSC3, SSC4, SSC6, and SSC11.
SSC6 revealed QTL in both populations at different positions, indicating the segregation of at least two QTL. The results
confirm the previously proposed polygenic inheritance of the inverted teat defect and, for the first time, point to genomic
regions harboring relevant genes. The investigation revealed variation of the importance of QTL in the various populations
due to either differences in allele frequencies and statistical power or differences in the genetic background that modulates
the impact of the liability loci on the expression of the disease. The QTL study enabled us to name a number of plausible
positional candidate genes. The correspondence of QTL regions for the inverted teat defect and previously mapped QTL for teat
number are in line with the etiologic relationship of these traits. 相似文献
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Wimmers K Murani E Te Pas MF Chang KC Davoli R Merks JW Henne H Muraniova M da Costa N Harlizius B Schellander K Böll I Braglia S de Wit AA Cagnazzo M Fontanesi L Prins D Ponsuksili S 《Animal genetics》2007,38(5):474-484
Ten genes (ANK1, bR10D1, CA3, EPOR, HMGA2, MYPN, NME1, PDGFRA, ERC1, TTN), whose candidacy for meat-quality and carcass traits arises from their differential expression in prenatal muscle development, were examined for association in 1700 performance-tested fattening pigs of commercial purebred and crossbred herds of Duroc, Pietrain, Pietrain x (Landrace x Large White), Duroc x (Landrace x Large White) as well as in an experimental F(2) population based on a reciprocal cross of Duroc and Pietrain. Comparative sequencing revealed polymorphic sites segregating across commercial breeds. Genetic mapping results corresponded to pre-existing assignments to porcine chromosomes or current human-porcine comparative maps. Nine of these genes showed association with meat-quality and carcass traits at a nominal P-value of < or = 0.05; PDGFRA revealed no association reaching the P < or = 0.05 threshold. In particular, HMGA2, CA3, EPOR, NME1 and TTN were associated with meat colour, pH and conductivity of loin 24 h postmortem; CA3 and MYPN exhibited association with ham weight and lean content (FOM) respectively at P-values of < 0.003 that correspond to false discovery rates of < 0.05. However, none of the genes showed significant associations for a particular trait across all populations. The study revealed statistical-genetic evidence for association of the functional candidate genes with traits related to meat quality and muscle deposition. The polymorphisms detected are not likely causal, but markers were identified that are in linkage disequilibrium with causal genetic variation within particular populations. 相似文献
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Klaus Wimmers Eduard Murani Siriluck Ponsuksili Martine Yerle Karl Schellander 《Mammalian genome》2002,13(4):206-210
For evaluation of the suitability of Amplified Fragment Length Polymorphism (AFLP) for detection of quantitative trait loci
in farm animals, a combination of AFLP and selective genotyping has been applied as a rapid screening method for marker–QTL
associations. Focusing on loci affecting eye muscle area, six extreme discordant sib pairs were selected from a Duroc × Berlin
Miniature Pig F2 experimental cross and examined by using 48 AFLP primer combinations. Two prominent AFLP markers were converted into simple
codominant PCR markers (STS-Bo1 and STS-Bo3) and assigned to Sscr4 by physical and linkage mapping. Single marker analysis indicated association of the STS markers with
a putative QTL influencing eye muscle area. Interval mapping confirmed the presence of a significant QTL for eye muscle area
(Pgenomewide < 0.01) on the Sscr4, with STS-Bo1 being the closer marker. At the same location, significant effects (Pgenomewide < 0.01) on carcass length and backfat thickness were also detected. Our results demonstrate the capability of the combination
of AFLP analysis and selective genotyping as a method for detection of genome regions containing QTL in livestock. 相似文献
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