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51.
R. S. Singh S. K. Jain 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1971,41(2):79-84
Summary Surveys for polymorphisms in natural populations of A. barbata sampled in California grasslands had provided evidence for widespread monomorphism and rather localized polymorphic areas in the north coastal and San Francisco regions, based on a set of morphological and isoenzymatic marker loci. Since this species, like many other annuals, was introduced from the Mediterranean region during the Spanish mission period, a comparative study of the Canadian-Welsh collections of Avena species from the Mediterranean region was undertaken using various plant characters and starch gel electrophoresis to analyze variants for esterase, phosphatase and peroxidase systems. A total of 96 samples including 73 of A. barbata and 23 of A. hirtula were studied and the results were scored to compute the polymorphism indices. In both species, only 10 to 15 percent sites showed any significant degree of polymorphism of which a majority seemed to originate from localized regions in Italy and Turkey; a part of this observed lack of within-sample variation might be the result of small sample size. In general, the patterns of variation in A. barbata from the California surveys and the present analyses seemed to be very similar and raised some interesting questions on (a) the colonizing history of introduced materials (b) the factors underlying such marked patterns of geographical variation, and (c) the current evolutionary changes occurring in these two broad, disjunct areas of species distribution.
This work was supported in part by a grant from the National Science Foundation (GB 8627). 相似文献
Zusammenfassung Untersuchungen der Polymorphismen in natürlichen Populationen von A. barbata im kalifornischen Weideland hatten einerseits zum Nachweis eines weit verbreiteten Monomorphismus und andererseits streng lokalisierter polymorpher Bereiche in der nördlichen Küsten- und San Francisco-Region geführt, wobei eine Anzahl morphologischer und isoenzymatischer Markerloci zugrunde gelegt wurde. Da diese Art, wie viele andere Annuelle auch, während der spanischen Missionsperiode aus der Mittelmeerregion eingeführt wurde, wurde eine vergleichende Untersuchung der Canadian-Welsh-Sammlungen von Avena-Arten aus der Mittelmeerregion anhand verschiedener Merkmale der Pflanzen und der Stärkegelelektrophorese-Untersuchung auf Esterase-, Phosphatase- und Peroxydase-Systeme durchgeführt. Es wurde eine Gesamtheit von 96 Stichproben, bestehend aus 73 A. barbata und 23 A. hirtula, untersucht und die Ergebnisse zur Berechnung von Polymorphismus-Indices verwendet. In beiden Arten zeigten nur 10 bis 15% der Herkünfte einen signifikanten Polymorphismusgrad. Von ihnen scheint die Mehrzahl von lokalisierten Regionen in Italien und Griechenland abzustammen. Ein Teil des beobachteten Fehlens einer Variation innerhalb der Stichproben könnte eine Folge des geringen Stichprobenumfangs sein. Im allgemeinen scheinen die Variationsmuster der kalifornischen Untersuchungen und die der vorliegenden Analysen von A. barbata sehr ähnlich zu sein. Das führt zu einigen interessanten Fragen nach a) der Besiedelungsgeschichte des eingeführten Materials, b) den Faktoren, die derart auffallenden Mustern der geographischen Variation unterliegen und c) den laufenden evolutionären Änderungen, die in diesen beiden großen, voneinander getrennten Gebieten der Artverteilung auftreten.
This work was supported in part by a grant from the National Science Foundation (GB 8627). 相似文献
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Summary Laboratory studies to determine the tolerance of an isolate ofAlternaria tenuis causing a black core rot of mandarin oranges (Citrus reticulata) have shown that presence of low amounts of manganese, zinc, iron, copper, boron, and molybdenum in a synthetic medium stimulated growth and sporulation of the fungus. Concentrations of 20 ppm or more of copper sulphate, boric acid, and ammonium molybdate, however, caused significant reduction of growth. Conidia of the fungus were very sensitive to even low amounts of copper, zinc, and iron. Copper sulphate was most toxic to spore germination followed, in order of inhibitory effect, by zinc sulphate, ferrous sulphate, and boric acid. Ammonium molybdate and manganese sulphate were stimulatory to spore germination at 5 ppm. There was a positive correlation between percentage of spore germination and average length of germ tubes in different concentrations of various chemicals. The possibility of using copper sulphate, zinc sulphate, and ferrous sulphate as spray materials for trees and as dip for ripe fruits to prevent black core rot are discussed.Assoc. Professor and Research Assistant, respectively. Publication under journal series No. 27 from Experiment Station, U.P. Agricultural University. 相似文献
54.
Bacterial and animal viruses were inactivated by high voltage electrical discharges in water. The sensitivity of phages to the immediate component of this effect was correlated to the sensitivity to ultraviolet radiation. Transient electrical arcs in weak electrolytes also generated chemical compounds which were virucidal against phages T3, T5, and varphiX174 but were only slightly virucidal against phages T2 and T4. 相似文献
55.
T. P. Singh 《Cell and tissue research》1968,87(3):422-428
Summary The thyroid gland of Mystus vittatus and Esomus danricus showed seasonal activity in its epithelial cell height and radioiodine uptake. These two indices of activity agree in E. danricus. Under normal photoperiods both species exhibited two phases of optimum activity, one in April through May, the other in September. The active periods were characterized by high iodine uptake and increased follicular cell height. These peaks of activity alternated with more quiescent phases, minimal iodine uptake and low follicular epithelium. In specimens exposed to constant illumination the active and quiescent phases were advanced by two months. But for this change the general pattern of activity was the same as that in animals kept under normal photoperiodic conditions. Specimens subjected to total darkness showed a higher level of activity throughout the year in comparison to the other two groups. However, a distinct seasonal cycle, which was evident with normal photoperiods and continuous illumination, was absent in total darkness.I am greatly indebted to Dr. A. G. Sathyanesan, Banaras Hindu University, for his encouragement, and to Professor S. P. Ray-Chaudhuri, Banaras Hindu University, for providing laboratory facilities. 相似文献
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A bovine strain of myxovirus parainfluenza-3 (MP3) virus, designated S virus, was isolated from lung tissue collected from cattle with respiratory illness in 1963. The virus agglutinates mammalian and avian erythrocytes, and is sensitive to ether, sodium desoxycholate and trypsin. It grows in primary calf kidney, buffalo kidney, dog kidney, camel kidney and MS cell cultures. The S virus forms well-defined plaques in buffalo and calf kidney cells on the 5th or 6th day after inoculation. Examination of cell cultures following inoculation with S virus revealed giant cell formation, and introcytoplasmic and intranuclear inclusions. At 37 degrees C the virus titer dropped from 10(10.4) to 10(2.6) in 3 days. Virus was completely inactivated at 56 degrees C within 15 minutes. Growth-curve studies in tissue culture monolayer cells revealed a latent period of 10 hours. The intracellular virus titer was slightly lower than that of extracellular virus. The isolate was identified as MP3 virus by serum neutralization and hemagglutination-inhibition tests. Antibodies (HI) to S virus were shown to be present in a significant proportion of Egyptian cattle. The epidemiological significance of MP3 (bovine strain) virus in U.A.R. is discussed. 相似文献
60.
Inderbir Singh 《Cell and tissue research》1967,81(4):501-510
Summary The individual granules of enterochromaffin cells of normal and reserpine treated guinea pigs have been studied by staining slides of the duodenum first by an argentaffin method and subsequently by an argyrophile method. Some argentaffin cells can be shown to contain not only argentaffin granules, but also granules that are purely argyrophile. The relative number of such argentaffin cells is greatly increased following administration of reserpine, as depletion of their 5-hydroxytryptamine content converts argentaffin granules into purely argyrophile ones. On the basis of this finding it is confirmed that the argyrophile granule is merely an argentaffin granule depleted of its 5-HT content, and that the argyrophile (nonargentaffin) and the argentaffin cells represent different phases of a secretory cycle.This investigation was supported by a grant from the Indian Council of Medical Research. I am grateful to Ms. Ciba of India Ltd. for making available reserpine (Serpasil) and solvent for reserpine. It is a pleasure to thank Mr. Anand Parkash for technical assistance and Mr. M. L. Sharma for the photographs. 相似文献