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991.
992.
Michael J. Thomas Matthew L. Peterson Eric D. Chapman Alex R. Hearn Gabriel P. Singer Ryan D. Battleson A. Peter Klimley 《Environmental Biology of Fishes》2014,97(2):133-146
We conducted the first continuous shipboard tracking of southern Distinct Population Segment green sturgeon, Acipenser medirostris, in the Sacramento River. Tracking of adult green sturgeon occurred between river kilometer (rkm) 434.8 and 511.6, a section of the putative spawning grounds located near Red Bluff, California. The recorded positions of acoustically tagged green sturgeon were analyzed using First Passage Time analysis to determine differences in habitat use between suitable and non-suitable habitats. Classification and Regression Tree modeling was used to determine explanatory inputs attributable to above average habitat use. Green sturgeon exhibited above average habitat use at five sites, identified as potential spawning aggregate sites. Three types of movements (holding, milling, and directed) could be categorized from tracks. Lastly, we show that green sturgeon while on the spawning grounds exhibit a high degree of mobility throughout the spawning grounds, often making large movements between specific habitat units. Our study illustrates how the application of shipboard tracking can be useful for describing movement, behavior and habitat utilization at a spatial scale not achieved by stationary acoustic monitors. 相似文献
993.
Proteasomal turnover of p21Cip1 does not require p21Cip1 ubiquitination 总被引:12,自引:0,他引:12
The Cdk inhibitor p21Cip1 is an unstable protein. Pharmacologic inhibition of the proteasome increases the half-life of p21 from less than 30 min to more than 2 hr and results in the accumulation of p21-ubiquitin conjugates. To determine whether ubiquitination was required for proteasomal degradation of p21, we constructed mutant versions of p21 that were not ubiquitinated in vivo. Remarkably, these mutants remained unstable and increased in abundance upon proteasome inhibition, indicating that direct ubiquitination of p21 is not necessary for its turnover by the proteasome. The frequently observed correlation between protein ubiquitination and proteasomal degradation is insufficient to conclude that ubiquitination is a prerequisite for degradation. 相似文献
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Daniela S. Aliaga Goltsman Vincent J. Denef Steven W. Singer Nathan C. VerBerkmoes Mark Lefsrud Ryan S. Mueller Gregory J. Dick Christine L. Sun Korin E. Wheeler Adam Zemla Brett J. Baker Loren Hauser Miriam Land Manesh B. Shah Michael P. Thelen Robert L. Hettich Jillian F. Banfield 《Applied and environmental microbiology》2009,75(13):4599-4615
We analyzed near-complete population (composite) genomic sequences for coexisting acidophilic iron-oxidizing Leptospirillum group II and III bacteria (phylum Nitrospirae) and an extrachromosomal plasmid from a Richmond Mine, Iron Mountain, CA, acid mine drainage biofilm. Community proteomic analysis of the genomically characterized sample and two other biofilms identified 64.6% and 44.9% of the predicted proteins of Leptospirillum groups II and III, respectively, and 20% of the predicted plasmid proteins. The bacteria share 92% 16S rRNA gene sequence identity and >60% of their genes, including integrated plasmid-like regions. The extrachromosomal plasmid carries conjugation genes with detectable sequence similarity to genes in the integrated conjugative plasmid, but only those on the extrachromosomal element were identified by proteomics. Both bacterial groups have genes for community-essential functions, including carbon fixation and biosynthesis of vitamins, fatty acids, and biopolymers (including cellulose); proteomic analyses reveal these activities. Both Leptospirillum types have multiple pathways for osmotic protection. Although both are motile, signal transduction and methyl-accepting chemotaxis proteins are more abundant in Leptospirillum group III, consistent with its distribution in gradients within biofilms. Interestingly, Leptospirillum group II uses a methyl-dependent and Leptospirillum group III a methyl-independent response pathway. Although only Leptospirillum group III can fix nitrogen, these proteins were not identified by proteomics. The abundances of core proteins are similar in all communities, but the abundance levels of unique and shared proteins of unknown function vary. Some proteins unique to one organism were highly expressed and may be key to the functional and ecological differentiation of Leptospirillum groups II and III.To understand how microorganisms contribute to biogeochemical cycling, it is necessary to determine the roles of uncultivated as well as cultivated groups and to establish how these roles vary during ecological succession and when environmental conditions change. Shotgun genomic sequencing (metagenomics) has opened new opportunities for culture-independent studies of microbial communities. Examples include investigations of acid mine drainage (AMD) biofilm communities (4, 43, 75), symbiosis in a marine worm involving sulfur-oxidizing and sulfate-reducing bacteria (85), and enhanced biological phosphorous removal by sludge communities (32). From these genomic data sets, it has been possible to reconstruct aspects of the metabolism of individual organisms (32) and coexisting community members (29, 75) and to identify which organisms contribute community-essential functions (75). An interesting question relates to how differences in metabolic potential between organisms from the same lineage allow them to occupy distinct niches. Identification of potentially adaptive traits in closely related organisms is also important from an evolutionary perspective.Genomic data do not reveal how organisms alter their metabolisms in response to the presence of other organisms or environmental conditions. Proteomics methods for analysis of metabolic responses of isolates (16, 17, 42, 80, 81) have been extended to analyze the functioning of the dominant members of natural consortia (56, 69), with strain-level resolution (43, 82). In these studies, peptides are separated by liquid chromatography (LC) and identified by tandem mass spectrometry (MS-MS) through reference to appropriate genomic databases. Proteomic analysis is possible even if the genome sequences are not identical to those of the organisms present (24); however, missing sequence information reduces the resolution of such proteogenomic studies.Due to dominance by a few organism types, chemoautotrophic microbial AMD biofilms from Richmond Mine, Iron Mountain, CA, are tractable model systems used to develop cultivation-independent metagenomic and proteogenomic methods for analysis of community structure, function, and ecology (13). Acidophilic Leptospirillum bacteria dominate this AMD system (15), other AMD systems (54), and bioleaching systems used for recovery of metals (19, 53, 86). These bacteria play pivotal roles in sulfide mineral dissolution because they are iron oxidizers (53, 75), and ferric iron drives sulfide oxidation, leading to formation of metal-rich sulfuric acid solutions. According to a recent microscopy-based study (83), Leptospirillum group II are the first colonists in AMD biofilm communities whereas Leptospirillum group III generally appear later, sometimes partitioned within biofilm interiors. Because only Leptospirillum group III appear to be able to fix nitrogen, they may be keystone species in AMD ecosystems (75). This observation enabled the isolation of one representative, “Leptospirillum ferrodiazotrophum” (76). In prior work, we reported near-complete genome sequences of two Leptospirillum group II types (43, 65), but detailed functional annotations and metabolic analyses have not been published. Genomic data have been used to explore the metabolism of Leptospirillum bacteria in one biofilm community (56), but proteomic and genomic analyses of the same biofilm community have not been performed.Here, we report a near-complete genomic sequence for Leptospirillum group III, derived from a biofilm obtained from the UBA site within the Richmond Mine, Iron Mountain, CA; a detailed functional annotation of the genomes of Leptospirillum groups II and III; and a genomic and proteomic comparison of them. In addition, we report the sequence of an extrachromosomal plasmid associated with these organisms. This study represents the first comprehensive genomics-based analysis of the metabolism of bacteria in the Nitrospirae phylum and the first environmental community proteogenomic study where the genomic and proteomic data were derived from the same sample. We compared the proteomic profiles of three different biofilm communities to evaluate the importance of shared and unique genes and pathways in environmental adaptation. 相似文献
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998.
BRUNO FRÉDÉRICH H. DAVID SHEETS 《Biological journal of the Linnean Society. Linnean Society of London》2010,99(1):99-117
The evolution of body size, the paired phenomena of giantism and dwarfism, has long been studied by biologists and paleontologists. However, detailed investigations devoted to the study of the evolution of ontogenetic patterns shaping giant species are scarce. The damselfishes of the genus Dascyllus appear as an excellent model for such a study. Their well understood phylogeny reveals that large‐bodied species have evolved in two different clades. Geometric morphometric methods were used to compare the ontogenetic trajectories of the neurocranium and the mandible in both small‐bodied (Dascyllus aruanus and Dascyllus carneus; maximum size: 50–65 mm standard length) and giant (Dascyllus trimaculatus and Dascyllus flavicaudus; maximum size: 90–110 mm standard length) Dascyllus species. At their respective maximum body size, the neurocranium of the giant species is significantly shorter and have a higher supraoccipital crest relative to the small‐bodied species, whereas mandible shape variation is more limited and is not related to the ‘giant’ trait. The hypothesis of ontogenetic scaling whereby the giant species evolved by extending the allometric trajectory of the small‐bodied ones (i.e. hypermorphosis) is rejected. Instead, the allometric trajectories vary among species by lateral transpositions. The rate of shape changes and the type of lateral transposition also differ according to the skeletal unit among Dascyllus species. Differences seen between the two giant species in the present study demonstrate that giant species may appear by varied alterations of the ancestor allometric pattern. © 2010 The Linnean Society of London, Biological Journal of the Linnean Society, 2010, 99 , 99–117. 相似文献
999.
Virginia Devonshire Txomin Arbizu Björn Borre Michael Lang Alessandra Lugaresi Barry Singer Elisabetta Verdun di Cantogno Peter Cornelisse 《BMC neurology》2010,10(1):28
Background
Multiple sclerosis (MS) currently requires long-term treatment with disease-modifying drugs, administered parenterally up to once daily. The need for regular self-injection can be a barrier to treatment for many patients. Autoinjectors can help patients overcome problems or concerns with self-injection and could, therefore, improve treatment adherence. This study was performed to assess the suitability of a new electronic device for the subcutaneous (sc) administration of interferon (IFN) beta-1a, 44 mcg three times weekly, for relapsing MS. 相似文献1000.
Chantal A Rivera LaTausha Gaskin Georg Singer Jeff Houghton Monique Allman 《BMC physiology》2010,10(1):20