全文获取类型
收费全文 | 43504篇 |
免费 | 3305篇 |
国内免费 | 12篇 |
出版年
2023年 | 265篇 |
2022年 | 336篇 |
2021年 | 909篇 |
2020年 | 673篇 |
2019年 | 854篇 |
2018年 | 1138篇 |
2017年 | 1044篇 |
2016年 | 1405篇 |
2015年 | 1850篇 |
2014年 | 2054篇 |
2013年 | 2615篇 |
2012年 | 3059篇 |
2011年 | 2873篇 |
2010年 | 1949篇 |
2009年 | 1836篇 |
2008年 | 2283篇 |
2007年 | 2222篇 |
2006年 | 1993篇 |
2005年 | 1710篇 |
2004年 | 1756篇 |
2003年 | 1520篇 |
2002年 | 1440篇 |
2001年 | 1105篇 |
2000年 | 1006篇 |
1999年 | 870篇 |
1998年 | 506篇 |
1997年 | 392篇 |
1996年 | 366篇 |
1995年 | 356篇 |
1994年 | 300篇 |
1993年 | 281篇 |
1992年 | 456篇 |
1991年 | 450篇 |
1990年 | 398篇 |
1989年 | 384篇 |
1988年 | 333篇 |
1987年 | 343篇 |
1986年 | 292篇 |
1985年 | 285篇 |
1984年 | 223篇 |
1983年 | 219篇 |
1982年 | 177篇 |
1981年 | 141篇 |
1980年 | 136篇 |
1979年 | 189篇 |
1978年 | 141篇 |
1975年 | 141篇 |
1974年 | 167篇 |
1973年 | 157篇 |
1972年 | 129篇 |
排序方式: 共有10000条查询结果,搜索用时 140 毫秒
91.
Structure and growth pattern of the bizarre hemispheric prominence on the rostrum of the fossil beaked whale Globicetus hiberus (Mammalia,Cetacea, Ziphiidae)
下载免费PDF全文
![点击此处可从《Journal of morphology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Maïtena Dumont Vivian de Buffrénil Ismael Miján Olivier Lambert 《Journal of morphology》2016,277(10):1292-1308
The rostrum of most ziphiids (beaked whales) displays bizarre swollen regions, accompanied with extreme hypermineralisation and an alteration of the collagenous mesh of the bone. The functional significance of this specialization remains obscure. With the voluminous and dense hemispheric excrescence protruding from the premaxillae, the recently described fossil ziphiid Globicetus hiberus is the most spectacular case. This study describes the histological structure and interprets the growth pattern of this unique feature. Histologically, the prominence in Globicetus is made up of an atypical fibro‐lamellar complex displaying an irregular laminar organization and extreme compactness (osteosclerosis). Its development is suggested to have resulted from a protraction of periosteal accretion over the premaxillae, long after the end of somatic growth. Complex shifts in the geometry of this tissue are likely to have occurred during its accretion and no indication of Haversian remodeling could be found. X‐ray diffraction and Raman spectroscopy indicate that the bone matrix in the premaxillary prominence of Globicetus closely resembles that of the rostrum of the extant beaked whale Mesoplodon densirostris: apatite crystals are of common size and strongly oriented, but the collagenous meshwork within bone matrix seems to be extremely sparse. These morphological and structural data are discussed in the light of functional interpretations proposed for the highly unusual and diverse ziphiid rostrum. J. Morphol. 277:1292–1308, 2016. © 2016 Wiley Periodicals, Inc. 相似文献
92.
Sara Basconsuelo Herminda Reinoso Eugenia Lorenzo Rubén Bottini 《Plant Growth Regulation》1995,16(2):113-119
Morphological studies were carried out with peach flower buds collected monthly in 1989 and 1990, from two months before leaf fall (7 March) until two to three weeks before bloom (7/8 August). Chilled (2–4°C for 30 days) and unchilled buds were exposed to 20 to 25°C, 100% RH and continuous light. Gibberellin A3 (3 ng or 30 ng) was applied to some of the non-chilled cuttings at three days intervals. Then, 12, 19, and 26 days after they were planted, the buds were sampled and processed for histological studies. Cultured flower buds (chilled or unchilled) had accelerated anther and gynoecium morphogenesis after 12 days under controlled conditions, compared to buds processed immediately after collection from the field. Chilling treatment augmented the bud culture effect, while Gibberellin A3 applications to the excised buds retarded bud morphogenesis to a stage comparable to that of buds collected directly from the field. This, suggests that the comparatively high levels of Gibberellin A1/3 we previously found in mid winter [15, 18] could be at least one of the factors that controls floral bud dormancy by retarding anther and gynoecium development. 相似文献
93.
94.
Victor M. Luque-Almagro Verity J. Lyall Stuart J. Ferguson M. Dolores Roldán David J. Richardson Andrew J. Gates 《The Journal of biological chemistry》2013,288(41):29692-29702
Nitrogen is an essential nutrient for growth and is readily available to microbes in many environments in the form of ammonium and nitrate. Both ions are of environmental significance due to sustained use of inorganic fertilizers on agricultural soils. Diverse species of bacteria that have an assimilatory nitrate/nitrite reductase system (NAS) can use nitrate or nitrite as the sole nitrogen source for growth when ammonium is limited. In Paracoccus denitrificans, the pathway-specific two-component regulator for NAS expression is encoded by the nasT and nasS genes. Here, we show that the putative RNA-binding protein NasT is a positive regulator essential for expression of the nas gene cluster (i.e. nasABGHC). By contrast, a nitrogen oxyanion-binding sensor (NasS) is required for nitrate/nitrite-responsive control of nas gene expression. The NasS and NasT proteins co-purify as a stable heterotetrameric regulatory complex, NasS-NasT. This protein-protein interaction is sensitive to nitrate and nitrite, which cause dissociation of the NasS-NasT complex into monomeric NasS and an oligomeric form of NasT. NasT has been shown to bind the leader RNA for nasA. Thus, upon liberation from the complex, the positive regulator NasT is free to up-regulate nas gene expression. 相似文献
95.
Demet Tademir Ayegül Karaküük‐yidoan Mustafa Ulali Tuba Takin‐Tok Emne Eln Oru‐Emre Hasan Bayram 《Chirality》2015,27(2):177-188
A series of new chiral thiosemicarbazones derived from homochiral amines in both enantiomeric forms were synthesized and evaluated for their in vitro antiproliferative activity against A549 (human alveolar adenocarcinoma), MCF‐7 (human breast adenocarcinoma), HeLa (human cervical adenocarcinoma), and HGC‐27 (human stomach carcinoma) cell lines. Some of compounds showed inhibitory activities on the growth of cancer cell lines. Especially, compound 17b exhibited the most potent activity (IC50 4.6 μM) against HGC‐27 as compared with the reference compound, sindaxel (IC50 10.3 μM), and could be used as a lead compound to search new chiral thiosemicarbazone derivatives as antiproliferative agents. Chirality 27:177–188, 2015. © 2014 Wiley Periodicals, Inc. 相似文献
96.
97.
Eduardo León-Ruiz Purificación Alcázar Eugenio Domínguez-Vilches Carmen Galán 《Aerobiologia》2011,27(1):37-50
The present study sought to determine which of the common Poaceae species in the study area contribute most to the Poaceae
pollen season curve, and to determine the phenological behaviour of the species studied. The different floral phenophases
in thirty-three Poaceae species common in and around the city of Córdoba (SW Iberian Peninsula) were checked periodically
over the period 2004–2006. Results showed that longer phenological ranges were recorded in the coolest and wettest year, and
shorter ranges in the warmest and driest year. Moreover, ranges varied as a function of altitude: populations in lower-lying
areas flowered earlier than those at higher altitudes. The results, taken in conjunction with the findings of preliminary
research into potential pollen production, showed that probably only four of the Poaceae species studied—Dactylis glomerata, Lolium rigidum, Trisetaria panicea and Vulpia geniculata—were major contributors to the Poaceae airborne pollen curve. 相似文献
98.
Changes in quality of blood units containing one and a half or double amounts of glucose, stored at +4 degrees C for three weeks were analysed. An experimental preservative containing glucose and fructose (1 : 1) was also used. No other additives (purine or purine-nucleoside) were applied. A standard CPD preservative of the National Inst. of Haematology and Blood Transfusion was used as control. The pH, plasma free haemoglobin, K+ content, red blood cell (RBC) ATP and 2,3-DPG content, and RBC fragility index were determined in each sample. Increase of glucose concentration, the addition of fructose had a beneficial effect on blood pH, and on plasma free haemoglobin and K+ concentration. 150% glucose improved the 2,3-DPG maintenance in stored blood. 相似文献
99.
Using reversed-phase high-performance liquid chromatography with electrochemical detection we have demonstrated the occurrence of 5,6,7,8-tetrahydropterin and 5,6,7,8-tetrahydrobiopterin in Drosophila melanogaster. The former is the first time that has been detected in vivo. The identification has been based on the retention times, hydrodinamic voltagrams and the differential concentration in three strains of Drosophila melanogaster. Compared to the wild type, the Punch2 mutant has diminished levels of both pteridines, whereas Henna-recessive3 lacks completely tetrahydropterin and has increased levels of tetrahydrobiopterin, as expected according to their biochemical lesions. 相似文献
100.
Mónica López Fanarraga Javier Bellido Cristina Jaén Juan Carlos Villegas Juan Carlos Zabala 《PloS one》2010,5(1)
Microtubule-organizing centers recruit α- and β-tubulin polypeptides for microtubule nucleation. Tubulin synthesis is complex, requiring five specific cofactors, designated tubulin cofactors (TBCs) A–E, which contribute to various aspects of microtubule dynamics in vivo. Here, we show that tubulin cofactor D (TBCD) is concentrated at the centrosome and midbody, where it participates in centriologenesis, spindle organization, and cell abscission. TBCD exhibits a cell-cycle-specific pattern, localizing on the daughter centriole at G1 and on procentrioles by S, and disappearing from older centrioles at telophase as the protein is recruited to the midbody. Our data show that TBCD overexpression results in microtubule release from the centrosome and G1 arrest, whereas its depletion produces mitotic aberrations and incomplete microtubule retraction at the midbody during cytokinesis. TBCD is recruited to the centriole replication site at the onset of the centrosome duplication cycle. A role in centriologenesis is further supported in differentiating ciliated cells, where TBCD is organized into “centriolar rosettes”. These data suggest that TBCD participates in both canonical and de novo centriolar assembly pathways. 相似文献