全文获取类型
收费全文 | 1129篇 |
免费 | 158篇 |
专业分类
1287篇 |
出版年
2018年 | 13篇 |
2017年 | 9篇 |
2016年 | 20篇 |
2015年 | 29篇 |
2014年 | 36篇 |
2013年 | 40篇 |
2012年 | 51篇 |
2011年 | 49篇 |
2010年 | 29篇 |
2009年 | 36篇 |
2008年 | 40篇 |
2007年 | 37篇 |
2006年 | 33篇 |
2005年 | 35篇 |
2004年 | 35篇 |
2003年 | 31篇 |
2002年 | 28篇 |
2001年 | 27篇 |
2000年 | 35篇 |
1999年 | 31篇 |
1998年 | 23篇 |
1997年 | 12篇 |
1996年 | 9篇 |
1995年 | 18篇 |
1994年 | 20篇 |
1993年 | 11篇 |
1992年 | 24篇 |
1991年 | 23篇 |
1990年 | 26篇 |
1989年 | 18篇 |
1988年 | 24篇 |
1987年 | 21篇 |
1986年 | 18篇 |
1985年 | 24篇 |
1984年 | 18篇 |
1983年 | 9篇 |
1982年 | 11篇 |
1981年 | 13篇 |
1980年 | 11篇 |
1979年 | 20篇 |
1978年 | 16篇 |
1976年 | 11篇 |
1975年 | 18篇 |
1974年 | 19篇 |
1973年 | 20篇 |
1972年 | 16篇 |
1971年 | 9篇 |
1970年 | 10篇 |
1969年 | 13篇 |
1968年 | 9篇 |
排序方式: 共有1287条查询结果,搜索用时 0 毫秒
991.
992.
Resource selection,utilization and seasons influence spatial distribution of ungulates in the western Serengeti National Park 下载免费PDF全文
John Bukombe Andrew Kittle Ramadhan Senzota Simon Mduma John Fryxell Anthony R.E. Sinclair 《African Journal of Ecology》2018,56(1):3-11
Understanding herbivore selection and utilization of vegetation types is fundamental to conservation of multispecies communities. We tested three hypotheses for how ungulate species select their habitats and how this changes with season: first, resources are distributed as a mosaic of patches so that ungulates are also distributed patchily; this distribution reflects habitat selection, which changes with season, the different ungulates behaving differently. Second, resources become scarcer in the dry season relative to those in the wet season. If interspecific competition prevails, then all species should show a contraction of habitats chosen. Third, if predation is limiting, competition will be minimal, and hence, habitat selection by herbivores will not differ between seasons. We used frequencies of occurrence in four common vegetation types in western Serengeti National Park to determine selection coefficients and utilization patterns and Chi‐square analysis to test the hypotheses. The results showed that selection changes differently in each species, agreeing with the first hypothesis. Herbivores did not all become more selective, as predicted by the competition hypothesis, nor did selection remain the same across seasons, as predicted by the predation hypothesis. These results can be useful in constructing habitat suitability maps for ungulate species with special conservation needs. 相似文献
993.
Acetylation of the C terminus of Ku70 by CBP and PCAF controls Bax-mediated apoptosis 总被引:9,自引:0,他引:9
Cohen HY Lavu S Bitterman KJ Hekking B Imahiyerobo TA Miller C Frye R Ploegh H Kessler BM Sinclair DA 《Molecular cell》2004,13(5):627-638
Apoptosis is a key tumor suppression mechanism that can be initiated by activation of the proapoptotic factor Bax. The Ku70 DNA end-joining protein has recently been shown to suppress apoptosis by sequestering Bax from mitochondria. The mechanism by which Bax is regulated remains unknown. Here, we identify eight lysines in Ku70 that are targets for acetylation in vivo. Five of these, K539, K542, K544, K533, and K556, lie in the C-terminal linker domain of Ku70 adjacent to the Bax interaction domain. We show that CBP and PCAF efficiently acetylate K542 in vitro and associate with Ku70 in vivo. Mimicking acetylation of K539 or K542 or treating cells with deacetylase inhibitors abolishes the ability of Ku70 to suppress Bax-mediated apoptosis. We demonstrate that increased acetylation of Ku70 disrupts the Ku70-Bax interaction and coincides with cytoplasmic accumulation of CBP. These results shed light on the role of acetyltransferases as tumor suppressors. 相似文献
994.
Mason HY Lloyd C Dice M Sinclair R Ellis W Powers L 《Biosensors & bioelectronics》2003,18(5-6):521-527
Quick and accurate detection of microbial contamination is accomplished by a unique combination of leading edge technologies described in this and the accompanying article. Microbe capture chips, used with a prototype fluorescence detector, are capable of statistically sampling the environment for pathogens (including spores), identifying the specific pathogens/exotoxins, and determining cell viability where appropriate. 相似文献
995.
Kasapidou E Enser M Wood JD Richardson RI Wilkinson RG Sinclair LA 《Animal : an international journal of animal bioscience》2009,3(4):516-526
In order to determine the effect of dietary vitamin E level and basal diet on vitamin E status, performance and tissue fatty acid content, five groups of eight Suffolk × Charollais wether lambs with an initial live weight of 28.4 (s.d. 1.6) kg were allocated to one of five concentrate-based diets supplemented with all-rac-α-tocopheryl acetate to contain 30 mg (C-30), 60 mg (C-60), 120 mg (C-120), 250 mg (C-250) or 500 mg (C-500) α-tocopheryl acetate/kg dry matter (DM), for 63 days. Two additional groups of eight lambs entered the study at 31.2 (s.d. 3.3) kg and were fed grass silage and 400 g/day concentrate for 56 days, with the whole diet providing the equivalent of 60 mg (S-60) or 500 mg (S-500) α-tocopheryl acetate/kg DM. Lambs were weighed and blood samples obtained by venipuncture weekly. Dietary vitamin E level did not affect performance (P > 0.05), but lambs fed grass silage grew more slowly (P < 0.001) and had a higher (P < 0.001) feed conversion ratio (kg feed/kg gain) than those fed concentrates. At day 0 plasma α-tocopherol concentrations were 0.8 μg/ml and did not differ between treatments (P > 0.05). Plasma α-tocopherol concentrations then decreased in all lambs except for those fed S-500, which increased, and at slaughter were (μg/ml) 0.07, 0.23, 0.39, 0.76 and 1.57 in C-30, C-60, C-120, C-250 and C-500 and 1.18 and 1.93 in S-60 and S-500, respectively. At slaughter, muscle and liver α-tocopherol concentrations were in the deficiency range for lambs fed C-30, C-60 or C-120, whereas plasma creatine kinase and tissue polyunsaturated fatty acids were unaffected by dietary vitamin E level, but creatine kinase levels were higher (P < 0.05) and glutathione peroxidise levels lower (P < 0.001) in lambs fed grass silage than concentrates alone. Muscle and liver α-tocopherol concentrations were 1.8- and 4.1-fold higher in lambs fed S-60 than C-60, but there was less of a difference between lambs fed S-500 or C-500 with muscle and liver differences of 0.4- and 0.7-fold, respectively. Tissue n-3 polyunsaturated fatty acid concentrations were higher (P < 0.05) and n-6 fatty acids lower in lambs receiving the grass silage compared to concentrate-based diets, but were not affected by dietary vitamin E level. It is concluded that lower plasma and tissue levels of α-tocopherol are present in lambs supplemented with all-rac-α-tocopheryl acetate on a concentrate compared to a mixed diet of silage and concentrates, and that normal growth can be achieved at tissue levels previously considered to represent deficiency. 相似文献
996.
Haem synthesis from exogenous 5-aminolaevulinate in cultured chick-embryo hepatocytes. Effects of inducers of cytochromes P-450. 下载免费PDF全文
S I Shedlofsky P R Sinclair H L Bonkovsky J F Healey A T Swim J M Robinson 《The Biochemical journal》1987,248(1):229-236
The effects of inducers of cytochrome P-450 on haem biosynthesis from 5-aminolaevulinate were examined by using cultured chick-embryo hepatocytes. Cultures treated with either 2-propyl-2-isopropylacetamide or 3-methylcholanthrene contained increased amounts of cytochrome P-450 and haem. After treatment for 3 h with 5-amino[4-14C]laevulinate, the relative amounts of radioactivity accumulating as haem corresponded to the relative amounts of total cellular haem, but not to increases in the amounts of cytochrome P-450. Treatment with 5-aminolaevulinate did not alter cellular haem or cytochrome P-450 concentrations in either control or drug-treated cultures. The mechanism of the enhanced accumulation of radioactivity in haem was investigated. Although 2-propyl-2-isopropylacetamide enhanced the uptake of 5-aminolaevulinate and increased the cellular concentration of porphobilinogen 1.5-fold, these changes did not account for the increases in haem radioactivity. The inducing drugs had no effect on the rates of degradation of radioactive haem, but appeared to enhance conversion of protoporphyrin into haem. This latter effect was shown by: (1) a decreased accumulation of protoporphyrin from 5-aminolaevulinate in cells treated with inducers, and (2) complete prevention of this decrease if the iron chelator desferrioxamine was present. We conclude that inducers of cytochrome P-450 may increase haem synthesis not only by increasing activity of 5-aminolaevulinate synthase, but also by increasing conversion of protoporphyrin into haem. 相似文献
997.
998.
Hélène Marrou Jaumer J. Ricaurte Michel E. Ghanem José A. Clavijo Michelangeli Lamiae Ghaouti Idupulapati M. Rao Thomas R. Sinclair 《Physiologia plantarum》2018,162(1):109-122
Nitrogen (N) accumulation in legumes is one of the main determinants of crop yield. Although N accumulation from symbiotic nitrogen fixation or N absorption from the soil has been widely investigated, there is no clear consensus on timing of the beginning of N accumulation and the termination of N accumulation and the physiological events that may be associated with these two events. The analyses conducted in this study aimed at identifying the determinant of N accumulation in two grain legume species. Nitrogen accumulation dynamics and mass accumulation and development stages were recorded in the field for several genotypes of common bean (Phaseolus vulgaris) and faba bean (Vicia faba) under different growing conditions. This study showed that during the vegetative stages, N accumulation rate was correlated with mass accumulation rate. However, the maximum accumulation of N did not correspond to the time of the maximum mass accumulation. In fact, for both species, N accumulation was found to persist in seed growth. This challenges a common hypothesis that seed growth causes a decrease in N accumulation because of a shift of the photosynthate supply to support the seed growth. Even more surprising was the shift of the active accumulation of N in faba bean to late in the growing season as compared with common bean. N accumulation by faba bean only was initiated at high rates very late in vegetative growth and persisted at high rates well into seed fill. 相似文献
999.
We determined the complete nucleotide sequence of the chloroplast genome of the leptosporangiate fern, Adiantum capillus-veneris L. (Pteridaceae). The circular genome is 150,568 bp, with a large single-copy region (LSC) of 82,282 bp, a small-single copy region (SSC) of 21,392 bp and inverted repeats (IR) of 23,447 bp each. We compared the sequence to other published chloroplast genomes to infer the location of putative genes. When the IR is considered only once, we assigned 118 genes, of which 85 encode proteins, 29 encode tRNAs and 4 encode rRNAs. Four protein-coding genes, all four rRNA genes and six tRNA genes occur in the IR. Most (57) putative protein-coding genes appear to start with an ATG codon, but we also detected five other possible start codons, some of which suggest tRNA editing. We also found 26 apparent stop codons in 18 putative genes, also suggestive of RNA editing. We found all but one of the tRNA genes necessary to encode the complete repertoire required for translation. The missing trnK gene appears to have been disrupted by a large inversion, relative to other published chloroplast genomes. We detected several structural rearrangements that may provide useful information for phylogenetic studies. 相似文献
1000.
The modulated polarographic technique of O2 detection was applied to Chlorella to study the rate-limiting thermal reaction between Photosystem II and O2 evolution. From an analysis of the operation of the polarograph at different frequencies, it was concluded that a first order thermal reaction of rate constant 305±20 (S.E.) s−1 was consistent with the results of 22 °C. When the algae were successively studied in solutions made up with 2H2O and H2O, a kinetic isotopic effect for the rate constant of 1.29±0.05 (S.E.) was found. This suggests that the rate limiting step does not involve the breaking of the O-H bond in water. A temperature study of the rate constant indicated an activation energy of 5.9±0.5 (S.E.) kcal·mole−1 and an entropy of activation of −25 cal·degree−1·mole−1. The linearity of the Arrhenius plot between 8 and 42 °C demonstrated that only one reaction was rate-limiting over this temperature range. 相似文献