Mitochondrial and Oxidative Stress Aspects in Hippocampus of Rats Submitted to Dietary n-3 Polyunsaturated Fatty Acid Deficiency After Exposure to Early Stress

Neurochemical Research - Chronic dietary long-chain polyunsaturated fatty acids (PUFAs) deficiency may lead to changes in cortex and hippocampus neuronal membrane phospholipids, and may be linked...     

The galactose‐binding lectin isolated from Bauhinia bauhinioides Mart seeds inhibits neutrophil rolling and adhesion via primary cytokines

In this study, the amino acid sequence and anti‐inflammatory effect of Bauhinia bauhinioides (BBL) lectin were evaluated. Tandem mass spectrometry revealed that BBL possesses 86 amino acid residues. BBL (1 mg/kg) intravenously injected in rats 30 min prior to inflammatory stimuli inhibited the cellular edema induced by carrageenan in only the second phase (21% – 3 h, 19% – 4 h) and did not alter the osmotic edema induced by dextran. BBL also inhibited carrageenan peritoneal neutrophil migration (51%), leukocyte rolling (58%) and adhesion (68%) and the neutrophil migration induced by TNF‐α (64%). These effects were reversed by the association of BBL with galactose, demonstrating that the carbohydrate‐binding domain is essential for lectin activity. In addition, BBL reduced myeloperoxidase activity (84%) and TNF‐α (68%) and IL1‐β (47%) levels. In conclusion, the present investigation demonstrated that BBL contains highly homologous isolectins, resulting in a total of 86 amino acid residues, and exhibits anti‐inflammatory activity by inhibiting neutrophil migration by reducing TNF‐α and IL1‐β levels via the lectin domain. Copyright © 2015 John Wiley & Sons, Ltd.     

Establishing the evolutionary compatibility of potential sources of colonizers for overfished stocks: a population genomics approach

Identifying fish stock structure is fundamental to pinpoint stocks that might contribute colonizers to overfished stocks. However, a stock's potential to contribute to rebuilding hinges on demographic connectivity, a challenging parameter to measure. With genomics as a new tool, fisheries managers can detect signatures of natural selection and thus identify fishing areas likely to contribute evolutionarily compatible colonizers to an overfished area (i.e. colonizers that are not at a fitness disadvantage in the overfished area and able to reproduce at optimal rates). Identifying evolutionarily compatible stocks would help narrow the focus on establishing demographic connectivity where it matters. Here, we genotype 4723 SNPs in 616 orange roughy (Hoplostethus atlanticus) across five fishing areas off the Tasmanian coast in Australia. We ask whether these areas form a single genetic unit, and test for signatures of local adaptation. Results from amova , structure , discriminant analysis of principal components, bayesass and isolation by distance suggest that sampled locations are subjected to geneflow amounts that are above what is needed to establish ‘drift connectivity’. However, it remains unclear whether there is a single panmictic population or several highly connected populations. Most importantly, we did not find any evidence of local adaptation, suggesting that the examined orange roughy stocks are evolutionarily compatible. The data have helped test an assumption of the orange roughy management programme and to formulate hypotheses regarding stock demographic connectivity. Overall, our results demonstrate the potential of genomics to inform fisheries management, even when evidence for stock structure is sparse.     

Migrasomes: a new organelle of migrating cells

Cell migration is a multi-step process that involves the coordinated action of signaling networks, cytoskeletal dynamics and vesicular trafficking, leading to protrusion and adhesion at the leading edge of cells and contraction and detachment at their rear. In a recent paper in Cell Research, Ma et al. describe the biogenesis of a new exosome-like organelle — named migrasomes — that derive from retraction fibers at the rear of migrating cells and their potential roles in inter-cellular signaling.Cell migration is a complex process of fundamental importance in numerous physiological or pathological processes, including tissue development, repair and growth, as well as during cancer cell metastasis^1,2,3. Cells use multiple modes of migration depending on the properties of the extracellular matrix (ECM) upon which they move, for example its architecture (two or three dimensional), stiffness and composition, and also on cellular determinants, such as cell-cell and cell-ECM adhesiveness, traction forces and proteolysis⁴. However, in general cell migration is a cyclical process involving: 1) lamellipodial protrusion at the leading edge, 2) cell adhesion by integrin receptors, 3) creation of cell tension, 4) cell contraction, and 5) retraction of the cell rear end. Accomplishing these steps requires integration of signaling, cytoskeletal dynamics, adhesion and membrane trafficking^1,2,3,4.Although most research on migration focuses on events at the cell''s leading edge, the rear end (trailing edge) is equally important because without the coordinated disassembly of cell adhesions and the recycling of ECM receptors, cells would not move⁵. Long tubular cytoplasmic expansions called retraction fibers (fibrils) are observed at the rear end of forward migrating cells⁶, and also during abrupt detachment from substrates⁷. At the tips of these thin extensions, membrane shedding can occur, leaving integrin-containing vesicles attached to the substrate along the migratory path^7,8. Previously thought to be passively deposited cell remnants, Ma et al.⁹ provide evidence for the active transport of materials from the cell body to a specialized subcompartment within these fibers, called migrasomes. As the cell moves and the fibers retract, the migrasomes are released to function as a potentially new and structurally distinct category of exosome-like vesicles that transmit signals between migrating cells (Figure 1).Open in a separate windowFigure 1Migrasomes form within retraction fibers emerging from the trailing edge of migrating cells. They are released and deposited on the substrate as the fibers collapse. Migrasomes may represent a vector for inter-cellular communication as they can be engulfed by trailing cells, providing guidance cues or other information.Exosomes are micro-vesicles of endosomal origin thought to be released after fusion of multivesicular endosomes (MVBs) with the plasma membrane¹⁰. Exosomes, which contain cytosolic contents including miRNAs, as well as lipids and membrane receptors, are released from one cell and believed to bind and/or be taken up by another, providing a means of inter-cellular communication. Interestingly, exosomes have been shown to induce cell migration and invasion in cancer models¹¹. Proteomic analysis of purified migrasomes suggests that they are compositionally related to exosomes⁹, and in particular are enriched in tetraspanins, membrane protein markers of MVBs. However, unlike exosomes, the released migrasomes are large in diameter (0.5-1.2 μm) and contain variable numbers of small internal vesicles.To gain more insights into migrasome biogenesis, Ma et al. expressed and tracked GFP-tagged tetraspanin-4 (TSPAN4-GFP), a protein previously localized to retraction fibers⁷ and shown to be enriched in isolated migrasomes. By time-lapse microscopy of migrating cells⁹, migrasomes form as bulb-like structures at the tips of, or at the intersections between, retraction fibers. Importantly, these bulbs continue to grow in diameter, receiving cytosolic input continuously over an ∼2 h period as the migrating cell advances. When the retraction fibers break, migrasomes are released as a package of vesicles enclosed within a single limiting membrane. Eventually the migrasome dissociates from the substrate and is released into the medium, unless it is engulfed by an oncoming cell (see below).Not surprisingly, given their relationship with retraction fibers, migrasome formation is dependent on cell migration. Thus, the number of migrasomes formed per cell is increased when migration is enhanced (e.g., by coating surfaces with fibronectin, which stimulates cell adhesion and migration¹² or by knocking down SHARPIN, an endogenous inhibitor of β1 integrin activation¹³) and decreased when migration is inhibited (e.g., by treating cells with blebbistatin, a myosin II blocker¹⁴). Importantly, given cell type differences in migration and differences in migration on 2D vs 3D matrices and in vivo, the authors used TSPAN4-GFP transfection and scanning electron microscopy to identify migrasomes formed by multiple cell lines and under multiple conditions. They also identified externally deposited MVBs reminiscent of migrasomes by transmission electron microscopy in various mouse tissues, providing strong evidence for migrasome deposition under physiologically relevant conditions. Although morphologically reminiscent of MVBs, electron micrographs of migrasomes growing within retraction fibers show a collection of free vesicles and cytosolic content and not the deposition of intact MVBs. When the retraction fiber breaks, the released migrasome is surrounded by a single membrane, presumably derived from the surface membrane of the retraction fiber. This is important because the outer membrane of an MVB, unlike the retraction fiber membrane, would be topologically reversed exposing the cytoplasmic surface to the extracellular media.Migrasomes represent a distinct type of extracellular vesicle deposited through a unique mechanism and left in the tracks of migrating cells. Clearly, further work is necessary both to define the cellular components and potential signaling molecules that accumulate in growing bulbs along retraction fibers for release in migrasomes, and to identify the intracellular targeting signals and mechanisms that deposit them there. Most important is to understand the function of migrasomes. One clue comes from striking time-lapse videos showing that other cells following in the path of their depositors can take up migrasomes⁹. Which signals are activated upon contact with and/or internalization of deposited migrasomes? What inter-cellular messages might they be delivering? Could they serve as migratory benchmarks or guidance cues? The discovery of these extracellular vesicles raises many new questions and opens many avenues for future research.