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991.
Purification and analysis of murine 2-5A-dependent RNase 总被引:6,自引:0,他引:6
R H Silverman D D Jung N L Nolan-Sorden C W Dieffenbach V P Kedar D N SenGupta 《The Journal of biological chemistry》1988,263(15):7336-7341
2-5A-dependent RNase (RNase L, RNase F) is an enzyme which mediates effects of 2-5A (px(A2'p)nA; x = 2 or 3, n greater than or equal to 2) in cells. 2-5A binding activity present in mouse liver extracts was measured using a 32P-labeled 2-5A derivative. Analysis of Scatchard plots was consistent with a single noninteracting 2-5A binding site with a Ka of 2.5 X 10(10) M-1. Similarly, affinity labeling of proteins with a 32P-labeled 2-5A derivative revealed a single, high-affinity 2-5A-binding protein of Mr 80,000. This 2-5A-binding protein was the only mouse liver protein specifically and consistently eluted by 2-5A from an affinity resin consisting of core(2-5A) covalently attached to cellulose. The 2-5A-eluted protein could degrade polyuridylic acid but not polycytidylic acid. Furthermore, when the 2-5A-eluted protein was electrophoresed into a polyuridylic acid-containing, nondenaturing gel, a band of degraded polyuridylic acid was demonstrated after incubation with 2-5A. There was no band of degraded polyuridylic acid when the elution was performed either in the absence of oligonucleotide or in the presence of low amounts of a closely related analog of 2-5A, p3I2'pA2'pA. Therefore, the Mr 80,000 2-5A-binding protein and the 2-5A-dependent RNase were almost certainly the same protein. Finally, the Mr 80,000 2-5A-binding protein was purified to homogeneity by electroelution from a polyacrylamide gel. 相似文献
992.
M Silverman 《Stain technology》1986,61(3):135-137
Currently available mounting media require solvent evaporation for hardening. This process is slow and often is complicated by the formation of air spaces under the coverslip. Light-polymerizing plastics are introduced as histologic mounting media that eliminate these problems because they harden quickly when exposed to ultraviolet light and, therefore, do not depend upon solvent evaporation. These light-polymerizing plastics are easy to use, permit rapid and controlled hardening, and eliminate the formation of air spaces under the coverslip. The optical characteristics are satisfactory for light and fluorescence microscopy. 相似文献
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M Silverman 《Biochimica et biophysica acta》1976,457(3-4):303-351
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997.
Silverman GJ 《Arthritis research & therapy》2010,12(5):144
In systemic lupus erythematosus, the forces responsible for disease initiation and self-perpetuation in these clinically heterogeneous
populations remain poorly understood. Recent studies of the TAM (Tyro3, Axl and MerTK) family of receptor tyrosine kinases
may lead to a better understanding of the fundamental control system responsible for the clearance of apoptotic cells and
the regulation of inflammation. In a recent report, serum levels of the TAM ligand, Protein S, was found to correlate with
certain disease manifestations and with C3 and C4 levels. Protein S levels could provide a quantitative clinical biomarker
but it remains to be determined whether this factor directly affects disease activity. 相似文献
998.
Georgette Stern Julia Beel Béla Suki Mike Silverman Jenny Westaway Mateja Cernelc David Baldwin Urs Frey 《PloS one》2009,4(7)
Background
Control of breathing, heart rate, and body temperature are interdependent in infants, where instabilities in thermoregulation can contribute to apneas or even life-threatening events. Identifying abnormalities in thermoregulation is particularly important in the first 6 months of life, where autonomic regulation undergoes critical development. Fluctuations in body temperature have been shown to be sensitive to maturational stage as well as system failure in critically ill patients. We thus aimed to investigate the existence of fractal-like long-range correlations, indicative of temperature control, in night time rectal temperature (Trec) patterns in maturing infants.Methodology/Principal Findings
We measured Trec fluctuations in infants every 4 weeks from 4 to 20 weeks of age and before and after immunization. Long-range correlations in the temperature series were quantified by the correlation exponent, α using detrended fluctuation analysis. The effects of maturation, room temperature, and immunization on the strength of correlation were investigated. We found that Trec fluctuations exhibit fractal long-range correlations with a mean (SD) α of 1.51 (0.11), indicating that Trec is regulated in a highly correlated and hence deterministic manner. A significant increase in α with age from 1.42 (0.07) at 4 weeks to 1.58 (0.04) at 20 weeks reflects a change in long-range correlation behavior with maturation towards a smoother and more deterministic temperature regulation, potentially due to the decrease in surface area to body weight ratio in the maturing infant. α was not associated with mean room temperature or influenced by immunizationConclusions
This study shows that the quantification of long-range correlations using α derived from detrended fluctuation analysis is an observer-independent tool which can distinguish developmental stages of night time Trec pattern in young infants, reflective of maturation of the autonomic system. Detrended fluctuation analysis may prove useful for characterizing thermoregulation in premature and other infants at risk for life-threatening events. 相似文献999.
Genomewide linkage scan of 409 European-ancestry and African American families with schizophrenia: suggestive evidence of linkage at 8p23.3-p21.2 and 11p13.1-q14.1 in the combined sample 下载免费PDF全文
Suarez BK Duan J Sanders AR Hinrichs AL Jin CH Hou C Buccola NG Hale N Weilbaecher AN Nertney DA Olincy A Green S Schaffer AW Smith CJ Hannah DE Rice JP Cox NJ Martinez M Mowry BJ Amin F Silverman JM Black DW Byerley WF Crowe RR Freedman R Cloninger CR Levinson DF Gejman PV 《American journal of human genetics》2006,78(2):315-333
We report the clinical characteristics of a schizophrenia sample of 409 pedigrees--263 of European ancestry (EA) and 146 of African American ancestry (AA)--together with the results of a genome scan (with a simple tandem repeat polymorphism interval of 9 cM) and follow-up fine mapping. A family was required to have a proband with schizophrenia (SZ) and one or more siblings of the proband with SZ or schizoaffective disorder. Linkage analyses included 403 independent full-sibling affected sibling pairs (ASPs) (279 EA and 124 AA) and 100 all-possible half-sibling ASPs (15 EA and 85 AA). Nonparametric multipoint linkage analysis of all families detected two regions with suggestive evidence of linkage at 8p23.3-q12 and 11p11.2-q22.3 (empirical Z likelihood-ratio score [Z(lr)] threshold >/=2.65) and, in exploratory analyses, two other regions at 4p16.1-p15.32 in AA families and at 5p14.3-q11.2 in EA families. The most significant linkage peak was in chromosome 8p; its signal was mainly driven by the EA families. Z(lr) scores >2.0 in 8p were observed from 30.7 cM to 61.7 cM (Center for Inherited Disease Research map locations). The maximum evidence in the full sample was a multipoint Z(lr) of 3.25 (equivalent Kong-Cox LOD of 2.30) near D8S1771 (at 52 cM); there appeared to be two peaks, both telomeric to neuregulin 1 (NRG1). There is a paracentric inversion common in EA individuals within this region, the effect of which on the linkage evidence remains unknown in this and in other previously analyzed samples. Fine mapping of 8p did not significantly alter the significance or length of the peak. We also performed fine mapping of 4p16.3-p15.2, 5p15.2-q13.3, 10p15.3-p14, 10q25.3-q26.3, and 11p13-q23.3. The highest increase in Z(lr) scores was observed for 5p14.1-q12.1, where the maximum Z(lr) increased from 2.77 initially to 3.80 after fine mapping in the EA families. 相似文献
1000.
Natalya Karol Claude Brodski Yuval Bibi Tehila Kaisman Michal Forberg Michal Hershfinkel Israel Sekler William F. Silverman 《Journal of cellular physiology》2010,224(3):567-574
Release of Zn2+ from presynaptic glutamatergic terminals has long been considered the principle challenge necessitating the existence of zinc homeostatic proteins (ZHP) in the mammalian nervous system. It is now known that neural cells also possess an intracellular zinc pool, termed here [Zn2+]i, which functions in a cell signaling context. A major challenge is characterizing the interaction of these two populations of zinc ions. To assess the relationship of this Zn2+ pool to cellular ZHP production, we employed immunofluorescence and immunoblot analysis to compare the expression of ZHP's ZnT‐1 and MT‐I/II in olfactory bulb and hippocampus of wild‐type and ZnT‐3 KO mice, which lack synaptic Zn2+. In both areas, the respective distribution and concentration of ZnT‐1 and MT‐I/II were identical in ZnT‐3 KO and control animals. We subsequently examined ZHP content in ZnT‐3 KO and WT mice treated with a membrane‐permeable Zn2+ chelator. In both olfactory bulb and hippocampus of the KO mice, the ZHP content was significantly reduced 15 h after chelation of [Zn2+]i compared to WT controls. Our findings support the conclusion that ZHP expression is regulated by crosstalk between synaptic and intracellular pools of Zn2+. J. Cell. Physiol. 224: 567–574, 2010. © 2010 Wiley‐Liss, Inc. 相似文献