Gas chromatographie determination of extracellular metabolites produced by baker’s yeast during glucose-induced acidification

Gas chromatographic separation of metabolites extruded during the medium acidification by resting baker’s yeast supplied with glucose is described. Silylation with bis(trimethylsilyl)trifluoroacetamide and trimethylchlorosilane and a direct chromatography on Chromosorb G-AW-DMCS with 2.5 % SE-52 in a programmed temperature interval of 80–220 °C (3 °C/min) made it possible to separate organic acids (lactic, pyruvic, succinic, glyceric, fumaric, malic), polyols (glycerol, arabinitol) and sugars (glucose). The yeast was found to extrude glycerol, lactic, malic, and succinic acid.     

Effect of H2O2-induced oxidative stress on some yeast membrane functions

This work was supported by the NATO Linkage Grant H TECH.LG 930 686 and by grant no. 204/93/2224 of the Grant Agency of the Czech Republic.