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Stephen A Bustin Jean-François Beaulieu Jim Huggett Rolf Jaggi Frederick SB Kibenge Pål A Olsvik Louis C Penning Stefan Toegel 《BMC molecular biology》2010,11(1):74
The conclusions of thousands of peer-reviewed publications rely on data obtained using fluorescence-based quantitative real-time
PCR technology. However, the inadequate reporting of experimental detail, combined with the frequent use of flawed protocols
is leading to the publication of papers that may not be technically appropriate. We take the view that this problem requires
the delineation of a more transparent and comprehensive reporting policy from scientific journals. This editorial aims to
provide practical guidance for the incorporation of absolute minimum standards encompassing the key assay parameters for accurate
design, documentation and reporting of qPCR experiments (MIQE précis) and guidance on the publication of pure 'reference gene'
articles. 相似文献
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Phylogeny and biogeography of ratite birds inferred from DNA sequences of the mitochondrial ribosomal genes 总被引:7,自引:2,他引:5
The origin of the flightless ratite birds of the southern continents has
been debated for over a century. Whether dispersal or vicariance
(continental breakup) best explains their origin depends largely on their
phylogenetic relationships. No consensus has been reached on this issue
despite many morphological and molecular studies. To address this question
further we sequenced a 2.8-kb region of mitochondrial DNA containing the
ribosomal genes in representative ratites and a tinamou. Phylogenetic
analyses indicate that Struthio (Africa) is basal and Rhea (South America)
clusters with living Australasian ratites. This phylogeny agrees with
transferrin and DNA hybridization studies but not with sequence analyses of
some protein-coding genes. These results also require reevaluation of the
phylogenetic position of the extinct moas of New Zealand. We propose a new
hypothesis for the origin of ratites that combines elements of dispersal
and vicariance.
相似文献
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