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121.
Franc-Christophe Baurens Stéphanie Bocs Mathieu Rouard Takashi Matsumoto Robert NG Miller Marguerite Rodier-Goud Didier MBéguié-A-MBéguié Nabila Yahiaoui 《BMC plant biology》2010,10(1):149
Background
Comparative sequence analysis of complex loci such as resistance gene analog clusters allows estimating the degree of sequence conservation and mechanisms of divergence at the intraspecies level. In banana (Musa sp.), two diploid wild species Musa acuminata (A genome) and Musa balbisiana (B genome) contribute to the polyploid genome of many cultivars. The M. balbisiana species is associated with vigour and tolerance to pests and disease and little is known on the genome structure and haplotype diversity within this species. Here, we compare two genomic sequences of 253 and 223 kb corresponding to two haplotypes of the RGA08 resistance gene analog locus in M. balbisiana "Pisang Klutuk Wulung" (PKW). 相似文献122.
O. Yu. Portnyagina O. V. Sidorova O. D. Novikova O. P. Vostrikova V. A. Khomenko T. F. Solov’eva 《Russian Journal of Bioorganic Chemistry》2010,36(6):713-721
Multiple antigenic peptides (MAPs) that included the common antigenic epitopes of porins from the outer membranes (OM) of
bacteria from the Yersinia genus (Y. pseudotuberculosis, Y. enterocolitica, and Y. pestis that are pathogenic for humans) were synthesized. Mice of the BALB/c line were immunized with these peptides, and antisera
to the peptides were obtained. It was demonstrated by EIA that these sera interacted with the porins that were isolated from
the OM of pathogenic Yersinia. MAPs were shown to be bound to the antibodies in the blood sera of rabbits immunized with the individual porins and to the
antibodies in the blood sera of humans suffering from intestinal yersiniosis and pseudotuberculosis. 相似文献
123.
Sidorova MV Molokoedov AS Kudriavtseva EV Baldin MI Frid DA Ovchinnikov MV Bespalova ZhD Bushuev VN 《Bioorganicheskaia khimiia》2006,32(2):151-160
Two variants of the synthesis of tridecapeptide alloferon, the active principle of antiviral preparation allokine-alpha, were developed on the basis of fragment condensation in solution or on the Merrifield resin. The solid phase variant of the synthesis was shown to be more technological; it allows the preparation of the product at a higher total yield (40% vs. 17% for conventional synthesis in solution from the starting derivatives of the C-terminal dipeptide). The by-products formed during the synthesis of alloferon were identified. 相似文献
124.
Morphological (plant height and vegetative biomass amount) and symbiotic (number of nodules and nitrogenase activity) traits of six symbiotic pea mutants and the original cultivar Rondo were studied at different vegetation periods. Of the mutants studied, one (K10a) was supemodular and the remaining five (K1a, K2a, K5a, K7a, and K27a) were hypemodular. Essential distinctions in the absolute values and time course of the changes in individual morphological and symbiotic traits of different pea mutants were demonstrated. The supemodular type is inferior to the original cultivar in plant height and production of vegetative biomass, but exceeds it in nodulation and nitrogen fixation. The hypemodular mutants either surpass the original cultivar with respect to the production capacity or display similar results. The symbiotic traits-number of nodules and nitrogen fixation activity--of these mutants are higher compared with the Rondo cultivar. The mutants K1a, K2a, and K27a were demonstrated to be useful in breeding pea for an increase in nitrogen fixation. 相似文献
125.
126.
Sidorova M. V. Dudkina U. S. Avdeev D. V. Palkeeva M. E. Molokoedov A. S. Ovchinnikov M. V. Azmuko A. A. Grechishnikov S. B. Kudryavtseva E. V. Bushuev V. N. Arefieva T. I. 《Russian Journal of Bioorganic Chemistry》2020,46(4):520-529
Russian Journal of Bioorganic Chemistry - The solid phase synthesis (SPS) of the ingramon peptide antagonist of the human monocyte chemoattractant protein-1... 相似文献
127.
Sidorova NN Kurchashova SY Yarahmedov TY Ziganshin RH Kuimov AN 《Molecular and cellular biochemistry》2011,352(1-2):231-238
Mannose-binding lectin was identified as a substrate of tankyrase 2, an enzyme that catalyzes poly(ADP-ribosyl)ation. The endogenous tankyrase 2 was isolated out of cytoplasm of human embryonic kidney cells. It was bound to a soluble complex of at least two other proteins; they were identified using specific antibodies and other approaches as keratin 1 and mannose-binding lectin. Using immunoblot analysis and radioactive labeling, we detected tankyrase-2-dependent poly(ADP-ribosyl)ation of mannose-binding lectin. In the presence of NAD(+), the complex of keratin 1 and lectin was dissociated, what was recorded during elution of its separate components out of affinity columns and by decrease of their apparent molecular masses during gel-filtration. Tankyrase 2 also inhibited the carbohydrate-binding function of the lectin. The latter effect was observed using mannose-binding lectin out of human serum, which is free from keratin 1. As a result of tankyrase-2 activity, the lectin lost its affinity to mannan-agarose. The discovery of this new biochemical mechanism justifies further analysis of its physiological and medical significance. 相似文献
128.
Chandok GS Kapoor KK Brick RM Sidorova JM Krasilnikova MM 《Nucleic acids research》2011,39(6):2103-2115
Many mutation events in microsatellite DNA sequences were traced to the first embryonic divisions. It was not known what makes the first replication cycles of embryonic DNA different from subsequent replication cycles. Here we demonstrate that an unusual replication mode is involved in the first cycle of replication of DNA introduced in mammalian cells. This alternative replication starts at random positions, and occurs before the chromatin is fully assembled. It is detected in various cell lines and primary cells. The presence of single-stranded regions increases the efficiency of this alternative replication mode. The alternative replication cannot progress through the A/T-rich FRA16B fragile site, while the regular replication mode is not affected by it. A/T-rich microsatellites are associated with the majority of chromosomal breakpoints in cancer. We suggest that the alternative replication mode may be initiated at the regions with immature chromatin structure in embryonic and cancer cells resulting in increased genomic instability. This work demonstrates, for the first time, differences in the replication progression during the first and subsequent replication cycles in mammalian cells. 相似文献
129.
Vibeke Secher Dam Donna MB Boedtkjer Christian Aalkjaer Vladimir Matchkov 《Channels (Austin, Tex.)》2014,8(4):361-369
The presence of Ca2+-activated Cl– currents (ICl(Ca)) in vascular smooth muscle cells (VSMCs) is well established. ICl(Ca) are supposedly important for arterial contraction by linking changes in [Ca2+]i and membrane depolarization. Bestrophins and some members of the TMEM16 protein family were recently associated with ICl(Ca). Two distinct ICl(Ca) are characterized in VSMCs; the cGMP-dependent ICl(Ca) dependent upon bestrophin expression and the ‘classical’ Ca2+-activated Cl– current, which is bestrophin-independent. Interestingly, TMEM16A is essential for both the cGMP-dependent and the classical ICl(Ca). Furthermore, TMEM16A has a role in arterial contraction while bestrophins do not. TMEM16A’s role in the contractile response cannot be explained however only by a simple suppression of the depolarization by Cl– channels. It is suggested that TMEM16A expression modulates voltage-gated Ca2+ influx in a voltage-independent manner and recent studies also demonstrate a complex role of TMEM16A in modulating other membrane proteins. 相似文献
130.
Sung Aeong Oh Trudie Allen Gyun Jang Kim Anna Sidorova Michael Borg Soon Ki Park David Twell 《The Plant journal : for cell and molecular biology》2012,72(2):308-319
The conserved Fused kinase plays vital but divergent roles in many organisms from Hedgehog signalling in Drosophila to polarization and chemotaxis in Dictyostelium. Previously we have shown that Arabidopsis Fused kinase termed TWO‐IN‐ONE (TIO) is essential for cytokinesis in both sporophytic and gametophytic cell types. Here using in vivo imaging of GFP‐tagged microtubules in dividing microspores we show that TIO is required for expansion of the phragmoplast. We identify the phragmoplast‐associated kinesins, PAKRP1/Kinesin‐12A and PAKRP1L/Kinesin‐12B, as TIO‐interacting proteins and determine TIO‐Kinesin‐12 interaction domains and their requirement in male gametophytic cytokinesis. Our results support the role of TIO as a functional protein kinase that interacts with Kinesin‐12 subfamily members mainly through the C‐terminal ARM repeat domain, but with a contribution from the N‐terminal kinase domain. The interaction of TIO with Kinesin proteins and the functional requirement of their interaction domains support the operation of a Fused kinase signalling module in phragmoplast expansion that depends upon conserved structural features in diverse Fused kinases. 相似文献