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51.
Journal of Plant Growth Regulation - Nitric oxide (NO) acts as a gaseous diffusible plant growth regulator. It plays an important role in growth and development of plants. Therefore, in present... 相似文献
52.
Qadir Sami Ullah Raja Vaseem Siddiqui Weqar A. Alyemeni Mohammed Nasser Ahmad Parvaiz 《Journal of Plant Growth Regulation》2021,40(4):1450-1465
Journal of Plant Growth Regulation - Increased dependence on thermal power has resulted in a significant increase in the generation of fly ash (FA), which exacerbates environmental... 相似文献
53.
运用植物基因工程手段构建琉璃苣BoD6D转化载体,为提高油料作物油份中γ-亚麻酸含量奠定基础。以琉璃苣基因组DNA为模板克隆BoD6D,构建酵母表达载体并转化酿酒酵母,对酵母进行诱导表达,提取脂肪酸后进行甲酯化反应,利用气相色谱分析脂肪酸含量;同时构建植物双元表达载体,经农杆菌介导通过蘸花法转化拟南芥,最后对转基因拟南芥通过抗性筛选及PCR进行鉴定。结果表明,BoD6D编码区不含有内含子,可以直接用于后续的功能研究;成功构建了酿酒酵母表达载体pYES2-BoD6D,气相色谱检测结果表明BoD6D在酵母中能够成功的将亚油酸催化生成γ-亚麻酸;成功构建了植物双元表达载体pBI121-BoD6D,卡那霉素抗性筛选和PCR鉴定结果表明已经成功获得BoD6D的拟南芥转化植株。通过以琉璃苣基因组DNA为模板比较方便的获得有功能的BoD6D用于转基因植物研究。 相似文献
54.
为了解黑龙江省植物花期对气温变化的响应趋势。利用1951~2007年采集于黑龙江省的188种植物标本信息,分析黑龙江省植物花期变化与年均温、上年均温和春季、夏季、秋季均温变化的关系。结果表明:黑龙江省42.0%以上植物的花期与气温间存在显著的相关性(P<0.05)。这些植物中,25%以上物种的相对采集时间指数与年均温、上年均温及各季节均温存在显著正相关(P<0.05)关系,且多为草本植物。其余草本植物及木本植物的相对采集时间指数与年均温、上年均温及各季节均温多呈显著负相关(P<0.05),如春季均温每升高1℃,木本植物花期平均提前12.65%。年均温、上年均温和春季、夏季、秋季均温每升高1℃,黑龙江省植物花期分别整体提前4.47%、7.08%、3.87%、5.84%和6.73%。花期对气温变化的响应,在短花期植物中相对明显。相较于春季、秋季及年均温,黑龙江省植物花期对夏季均温变化的响应更为敏感。 相似文献
55.
结肠癌(colon cancer,CC)是一种常见的恶性肿瘤,其发病率和死亡率均占癌症前列。根据解剖学位置,CC可分为左半结肠癌(left-sided colon cancer,LCC)和右半结肠癌(right-sided colon cancer,RCC),两者在临床特征上表现出较大的差异。近些年来,随着生物学技术和测序技术的发展,从多组学角度分析LCC和RCC分子特征和微环境差异的研究也越来越多,从而来揭示患者预后并指导其治疗。该文从基因突变、基因表达、miRNA表达、DNA甲基化、免疫微环境、共识分子亚型以及免疫治疗这几个方面来阐述LCC和RCC在分子特征和治疗差异上的研究进展。 相似文献
56.
Abbasi M. A. Nazir M. Aziz-ur-Rehman Siddiqui S. Z. Raza Hussain Zafar Ayesha Shah S. A. A. Shahid M. 《Russian Journal of Bioorganic Chemistry》2021,47(5):1086-1096
Russian Journal of Bioorganic Chemistry - In the present research work, a new series of N-(substituted-phenyl)-3-(4-phenyl-1-piperazinyl)propanamides were synthesized. The synthesis was initiated... 相似文献
57.
Govindan Nadar Rajivgandhi Chelliah Chenthis Kanisha Govindan Ramachandran Natesan Manoharan Ramzi A. Mothana Nasir A. Siddiqui Adnan J. Al-Rehaily Riaz Ullah Omer M. Almarfadi 《Saudi Journal of Biological Sciences》2021,28(3):1763-1769
In this study, the phytochemical, phenolic, flavonoid and bioactive compounds were successfully screened from crude extract of Sargassum wightii by LC-MS analysis after NIST interpretation. Bacterial growth inhibition study result was shown with 24 mm zone inhibition at 200 µg/mL concentration against P. aeruginosa. The increased phenolic content was much closed to gallic acid and the range was observed at 250 μg/mL concentration. In addition, flavonoid contents of the algae extract was indicated more significant with rutin at 200 μg/mL. In result, both the phenolic and flavonoid contents of the extract were more correlated with gallic acid and rutin. Further, the total anti-oxidant and DPPH radical scavenging activities were shown increased activity at 200 μg/mL concentrations. Furthermore, the excellent anti-bacterial alteration result was observed at 200 μg/mL concentration by minimum inhibition concentration. Therefore, the result was revealed that the marine algae Sargassum wightii has excellent phytochemical and anti-oxidant activities, and it has improved anti-bacterial activity against P. aeruginosa. 相似文献
58.
Mohammed Rafi Shaik Rabbani Syed Syed Farooq Adil Mufsir Kuniyil Mujeeb Khan Mohammed S. Alqahtani Jilani P. Shaik Mohammed Rafiq H. Siddiqui Abdulrahman Al-Warthan Mohammed A.F. Sharaf Abdelatty Abdelgawad Emad Mahrous Awwad 《Saudi Journal of Biological Sciences》2021,28(2):1196-1202
Due to their inexpensive and eco-friendly nature, and existence of manganese in various oxidation states and their natural abundance have attained significant attention for the formation of Mn3O4 nanoparticles (Mn3O4 NPs). Herein, we report the preparation of Mn3O4 nanoparticles using manganese nitrate as a precursor material by utilization of a precipitation technique. The as-prepared Mn3O4 nanoparticles (Mn3O4 NPs) were characterized by using X-ray powder diffraction (XRD), UV–Visible spectroscopy (UV–Vis), High-Resolution Transmission electron microscopy (HRTEM), Field emission scanning electron microscopy (FESEM), Thermal gravimetric analysis (TGA) and Fourier-transform infrared spectroscopy (FT-IR). The antimicrobial properties of the as-synthesized Mn3O4 nanoparticles were investigated against numerous bacterial and fungal strains including S. aureus, E. coli, B. subtilis, P. aeruginosa, A. flavus and C. albicans. The Mn3O4 NPs inhibited the growth of S. aureus with a minimum inhibitory concentration (MIC) of 40 μg/ml and C. albicans with a MIC of 15 μg/ml. Furthermore, the Mn3O4 NPs anti-cancer activity was examined using MTT essay against A549 lung and MCF-7 breast cancer cell lines. The Mn3O4 NPs revealed significant activity against the examined cancer cell lines A549 and MCF-7. The IC50 values of Mn3O4 NPs with A549 cell line was found at concentration of 98 µg/mL and MCF-7 cell line was found at concentration of 25 µg/mL. 相似文献
59.
实时定量PCR构建法夫酵母内参基因β-actin、 gpd、18S rRNA标准品质粒和标准曲线 总被引:1,自引:0,他引:1
为建立检测法夫酵母JMU-MVP14中虾青素合成相关基因在不同生长时期表达水平的实时定量PCR方法,构建法夫酵母JMU-MVP14的管家基因β-actin、gpd、18S rRNA的标准质粒,进行实时定量PCR,制作标准曲线及回归方程.β-actin基因标准曲线相关系数(R2)=0.9956,扩增效率(E) =96.93%;gpd基因标准曲线相关系数(R2) =0.9901,扩增效率(E) =93.78%;18S rRNA基因标准曲线相关系数(R2) =0.9981,扩增效率(E)=98.76%.3个基因片段的熔解曲线均呈单峰;扩增曲线呈典型的S型动力学曲线,指数期和平台期明显,为理想的熔解曲线和扩增曲线.用geNorm软件对三个管家基因的稳定性进行分析,三个基因的稳定性排序为β-actin> 18S rRNA> gpd,故β-actin和18S rRNA较适合作为研究法夫酵母JMU-MVP14定量实验的内参基因. 相似文献
60.
Abdullah R. Doaigey M.H. Al-Whaibi M.H. Siddiqui A.A. Al Sahli M.E. El-Zaidy 《Saudi Journal of Biological Sciences》2013,20(2):141-147
Two concentrations (10-5M and 10-3M) of both GA3 and 2,4-D were used as foliar spray to evaluate the response of date palm (Phoenix dactylifera L.) cv. Khedri seedlings. They affected some of the anatomical characteristics of the first leaf emerging after the beginning of the spray. The high concentration of GA3 increased the size of the midrib and its vascular bundle numbers. Both low and high concentrations of 2,4-D inhibited the formation of the midrib. 2,4-D in both low and high concentrations decreased the number of vessels in both protoxylem and metaxylem and also decreased their diameters, where as GA3 in low and high concentrations have less effect on the number of vessels and its diameters. GA3 in high concentration increased the number of vascular bundles in 1mm long of the leaf blade, while 2,4-D in low and high concentrations decreased their numbers. 10-3M of 2,4-D increased the size and layers of special hypodermal cells. 相似文献