全文获取类型
收费全文 | 174篇 |
免费 | 9篇 |
国内免费 | 4篇 |
出版年
2022年 | 2篇 |
2021年 | 3篇 |
2020年 | 4篇 |
2018年 | 4篇 |
2017年 | 1篇 |
2016年 | 9篇 |
2015年 | 9篇 |
2014年 | 14篇 |
2013年 | 8篇 |
2012年 | 6篇 |
2011年 | 13篇 |
2010年 | 6篇 |
2009年 | 10篇 |
2008年 | 9篇 |
2007年 | 13篇 |
2006年 | 7篇 |
2005年 | 10篇 |
2004年 | 4篇 |
2003年 | 5篇 |
2002年 | 5篇 |
2001年 | 2篇 |
2000年 | 5篇 |
1999年 | 2篇 |
1998年 | 5篇 |
1997年 | 1篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 3篇 |
1988年 | 2篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1983年 | 4篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1977年 | 1篇 |
1973年 | 2篇 |
1972年 | 1篇 |
排序方式: 共有187条查询结果,搜索用时 109 毫秒
81.
82.
83.
E. S. Naumova K. V. Dmitruk B. V. Kshanovskaya A. A. Sibirny G. I. Naumov 《Microbiology》2013,82(4):453-458
The thermotolerant strain 1-IR was isolated as a contaminant microflora of the industrial strain “Red” (the Netherlands) during the study of the baker’s yeast Saccharomyces cerevisiae. The strain was assigned to the species Ogataea parapolymorpha by sequencing the 26S rDNA D1/D2 domain. The strain 1-IR was shown to be capable of efficient glucose and xylose fermentation at an elevated temperature of 45°C. In this respect, the strain 1-IR surpassed the thermotolerant yeasts O. polymorpha CBS 4732, NCYC 495, and O. parapolymorpha DL1. The prospects of using the O. parapolymorpha yeasts as producers of biofuel from lignocellulose wastes of agricultural and woodworking industries is discussed. 相似文献
84.
André M Siqueira Lucas I Coutinho Rafael L Gurgel Willian CS Su Luiz M Carvalho Silvana G Benzecry Aline CC Alencar Márcia AA Alexandre Maria Gra?as C Alecrim Marcus VG Lacerda 《Memórias do Instituto Oswaldo Cruz》2014,109(5):540-545
Plasmodium vivax is the most widespread parasite causing malaria, being
especially prevalent in the Americas and Southeast Asia. Children are one of the
most affected populations, especially in highly endemic areas. However, there are
few studies evaluating the therapeutic response of infants with vivax malaria.
This study retrospectively evaluated the parasitaemia clearance in children
diagnosed with vivax malaria during the first five days of exclusive treatment
with chloroquine (CQ). Infants aged less than six months old had a significantly
slower parasitaemia clearance time compared to the group of infants and children
between six months and 12 years old (Kaplan-Meier survival analysis; Wilcoxon
test; p = 0.004). The impaired clearance of parasitaemia in younger children with
vivax malaria is shown for the first time in Latin America. It is speculated that
CQ pharmacokinetics in young children with vivax malaria is distinct, but this
specific population may also allow the detection of CQ-resistant parasites during
follow-up, due to the lack of previous immunity. 相似文献
85.
86.
Valentyna Y. Yatsyshyn Olena P. Ishchuk Andriy Y. Voronovsky Daria V. Fedorovych Andriy A. Sibirny 《Metabolic engineering》2009,11(3):163-167
Recombinant strains of the flavinogenic yeast Candida famata able to overproduce flavin mononucleotide (FMN) that contain FMN1 gene encoding riboflavin (RF) kinase driven by the strong constitutive promoter TEF1 (translation elongation factor 1α) were constructed. Transformation of these strains with the additional plasmid containing the FMN1 gene under the TEF1 promoter resulted in the 200-fold increase in the riboflavin kinase activity and 100-fold increase in FMN production as compared to the wild-type strain (last feature was found only in iron-deficient medium).Overexpression of the FMN1 gene in the mutant that has deregulated riboflavin biosynthesis pathway and high level of riboflavin production in iron-sufficient medium led to the 30-fold increase in the riboflavin kinase activity and 400-fold increase in FMN production of the resulted transformants. The obtained C. famata recombinant strains can be used for the further construction of improved FMN overproducers. 相似文献
87.
Uwe Thissen Suzan Wopereis Sjoerd AA van den Berg Ivana Bobeldijk Robert Kleemann Teake Kooistra Ko Willems van Dijk Ben van Ommen Age K Smilde 《BMC bioinformatics》2009,10(1):52
Background
In the fields of life sciences, so-called designed studies are used for studying complex biological systems. The data derived from these studies comply with a study design aimed at generating relevant information while diminishing unwanted variation (noise). Knowledge about the study design can be used to decompose the total data into data blocks that are associated with specific effects. Subsequent statistical analysis can be improved by this decomposition if these are applied on selected combinations of effects. 相似文献88.
The XI International Rotifer Symposium was held during 11–18 March, 2006 at the National Autonomous University of Mexico Campus Iztacala located at the North Mexico City (Mexico). These triennial international meetings, first organized in Austria by Late Ruttner-Kolisko in September 1976, are gradually becoming the focal point of discussion and collaboration from rotifer workers across the world. The present XI symposium was attended by 125 participants from 20 nations. During this meeting, different themes of rotifer research from morphology to molecular biology were considered. In addition, there were four invited lectures and four workshops covering different themes of the symposium. During the last 30 years, rotifer research has witnessed gradual shift from the conventional morphological taxonomy to molecular and evolutionary systematics. While the basic rotifer ecological studies continue today, applied areas such as ecotoxicology and aquaculture have taken key roles in the recent meetings. The international rotifer meetings provide ample opportunities not only for exchange of ideas and recent research, but also for material and in establishing inter-personal relationships. Over the last 30 years, the number of participants attending the rotifer meetings has increased. 相似文献
89.
90.
Nazarko VY Nazarko TY Farré JC Stasyk OV Warnecke D Ulaszewski S Cregg JM Sibirny AA Subramani S 《Autophagy》2011,7(4):375-385
Autophagy-related (Atg) pathways deliver cytosol and organelles to the vacuole in double-membrane vesicles called autophagosomes, which are formed at the phagophore assembly site (PAS), where most of the core Atg proteins assemble. Atg28 is a component of the core autophagic machinery partially required for all Atg pathways in Pichia pastoris. This coiled-coil protein interacts with Atg17 and is essential for micropexophagy. However, the role of Atg28 in micropexophagy was unknown. We used the yeast two-hybrid system to search for Atg28 interaction partners from P. pastoris and identified a new Atg protein, named Atg35. The atg35Δ mutant was not affected in macropexophagy, cytoplasm-to-vacuole targeting or general autophagy. However, both Atg28 and Atg35 were required for micropexophagy and for the formation of the micropexophagic apparatus (MIPA). This requirement correlated with a stronger expression of both proteins on methanol and glucose. Atg28 mediated the interaction of Atg35 with Atg17. Trafficking of overexpressed Atg17 from the peripheral ER to the nuclear envelope was required to organize a peri-nuclear structure (PNS), the site of Atg35 colocalization during micropexophagy. In summary, Atg35 is a new Atg protein that relocates to the PNS and specifically regulates MIPA formation during micropexophagy.Key words: Atg protein, peroxisome, micropexophagy, MIPA, nucleus 相似文献