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排序方式: 共有257条查询结果,搜索用时 93 毫秒
91.
Lourdes Ortiz-Fernández Güher Saruhan-Direskeneli Fatma Alibaz-Oner Sema Kaymaz-Tahra Patrick Coit Xiufang Kong Allan P. Kiprianos Robert T. Maughan Sibel Z. Aydin Kenan Aksu Gokhan Keser Sevil Kamali Murat Inanc Jason Springer Servet Akar Fatos Onen Nurullah Akkoc Nader A. Khalidi Amr H. Sawalha 《American journal of human genetics》2021,108(1):84-99
92.
Polymorphism in the acetylcholinesterase gene of Musca domestica L. field populations in Turkey 总被引:1,自引:0,他引:1
Acetylcholinesterase (AChE), encoded by the Ace gene, is the primary target of organophosphates (OPs) and carbamates (CBs) in insects. Ace mutations have been identified in OP and CB resistant strains of Musca domestica. In this study, the Ace gene was partially amplified and sequenced at amino acid positions 260, 342, and 407 to determine the frequencies of these mutations in housefly samples collected from farms and garbage disposal sites of 16 provinces in the Aegean and Mediterranean regions of Turkey. In addition, the percent remaining AChE activities in these samples were assayed by using three OPs (malaoxon, paraoxon, and dichlorvos) and one CB (carbaryl) compound as inhibitors. In all the analyzed samples, 13 different combinations at the three amino acid positions were identified and the L/V260-A/G342-F/Y407 combination was found in the highest frequency. No susceptible individual was detected. The highest mean percent remaining AChE activities were detected in the individuals having the L260-A/G342-F/Y407 genotype when malaoxon and paraoxon were used as inhibitors and in the individuals with the L260-A342-F/Y407 combination when dichlorvos and carbaryl were used as inhibitors. The obtained data were heterogeneous and there was no exact correlation between the molecular genetic background and the resistance phenotypes of the flies. The findings of this study at the molecular and biochemical levels indicate the presence of significant control problems in the field. 相似文献
93.
94.
Catherine Larose Emmanuel Prestat Sébastien Cecillon Sibel Berger Cédric Malandain Delina Lyon Christophe Ferrari Dominique Schneider Aurélien Dommergue Timothy M. Vogel 《PloS one》2013,8(11)
We investigated the interactions between snowpack chemistry, mercury (Hg) contamination and microbial community structure and function in Arctic snow. Snowpack chemistry (inorganic and organic ions) including mercury (Hg) speciation was studied in samples collected during a two-month field study in a high Arctic site, Svalbard, Norway (79°N). Shifts in microbial community structure were determined by using a 16S rRNA gene phylogenetic microarray. We linked snowpack and meltwater chemistry to changes in microbial community structure by using co-inertia analyses (CIA) and explored changes in community function due to Hg contamination by q-PCR quantification of Hg-resistance genes in metagenomic samples. Based on the CIA, chemical and microbial data were linked (p = 0.006) with bioavailable Hg (BioHg) and methylmercury (MeHg) contributing significantly to the ordination of samples. Mercury was shown to influence community function with increases in merA gene copy numbers at low BioHg levels. Our results show that snowpacks can be considered as dynamic habitats with microbial and chemical components responding rapidly to environmental changes. 相似文献
95.
Batuhan Yaman Yakın Mustafa Koyun Mert Gürkan Sibel Hayretdağ Cemal Varol Tok 《Zoology in the Middle East.》2013,59(4):332-338
This study presents data on age, growth and longevity of a population of Neurergus strauchii in Eastern Anatolia (Bingöl, Turkey) based on skeletochronological data. The phalangeal diaphyseal cross-sections of 54 individuals studied (12♂, 42♀) showed that females are on average older than males: the ages ranged from 6 to 14 years, with an average age of 8.8 years in males and 10.9 years in females. Sexual maturity is reached at an age of 4-5 years in both sexes. The slow growth and the longevity make the species vulnerable. 相似文献
96.
?zlem ?zbek Belgin G??men Ta?k?n Sibel Keskin ?an Vehbi Eser Orhan Arslan 《Plant Systematics and Evolution》2011,296(1-2):121-135
Gliadin polymorphism in 19 landrace populations of Turkish cultivated emmer wheat [Triticum turgidum L. ssp. dicoccon (Schrank) Thell.] was assessed using the aluminum lactic acid-polyacrylamide gel electrophoresis (A-PAGE) technique. Being a source of useful genes, landraces of wheat represent one of the most important genetic resources available to breeders for present and future genetic improvement of wheat. This is the first genetic characterization of these 19 Turkish emmer wheat landrace populations collected from their main cultivation areas. Considerably high amounts of variation were detected within and among the populations. A total of 27 alleles (n a) were identified among all analyzed populations, 10 of them being unique to populations?C, D, H, K, L, M, and N. The highest allele number (n a?=?7) was observed in populations?A and L, whereas the lowest number of alleles (n a?=?3) was observed in populations?F, G, and U. The mean number of effective alleles (n ae) was 12.33, and the mean values of gene diversity, genetic differentiation, and gene flow between populations were H e?=?0.92, F ST?=?0.296, and N m ?=?0.60, respectively. Certain gliadins closely linked to dough quality, such as ??-45 and ??-35, were found in 13 and 18 of the populations, respectively. According to Pearson??s correlation coefficient values, gene diversity estimates had strong positive correlation (r P?=?0.510; p?=?0.026 at <0.05%) with latitude. The rest of the genetic data (n a and n ae) obtained in the present study showed no correlation with geographic (altitude, latitude, and longitude) or climatic factors (temperature and annual rainfall). Principal component analysis was performed to explain spatial genetic variation, revealing 90.044% of total genetic variation in three components. Results obtained from this study can effectively be used in developing more efficient breeding programs to improve wheat genotypes, and to direct genetic resource conservation studies. 相似文献
97.
doi: 10.1111/j.1741‐2358.2010.00401.x The clinical evaluation of the oral status in Alzheimer‐type dementia patients Background: To evaluate the oral health status in patients with Alzheimer dementia (AD), and the association of the disease severity with the oral findings. Methods: The study was conducted on the study group (31 AD patients) and the control group (47 healthy volunteers) from the Neurology Department of the Institute. Cognitive status was evaluated with the MMSE scoring system. Oral parameters, such as decaying, missing, filled teeth (DMFT) index and teeth present (PT) were evaluated in the patients. Oral hygiene status, denture status and mucosal lesions, including denture‐induced stomatitis were also investigated. Results: The study was conducted on the study group (31 AD patients) and the control group (47 healthy volunteers). Tooth brushing and denture cleaning were irregular in 22 of 31 (70%) patients with AD. The ratio of the subjects who forgot to remove their denture during the night was significantly higher in the study group than in the control group (p < 0.001). In this study, decreased cognitive functions in AD patients have been demonstrated to result in a deterioration of denture care and increased denture‐related mucosal lesions. Conclusions: These findings were considered due to decreased denture care including the non‐removal of the denture in the night in the patients with decreased cognitive functions. 相似文献
98.
Shirinzadeh H Altanlar N Yucel N Ozden S Suzen S 《Zeitschrift für Naturforschung. C, Journal of biosciences》2011,66(7-8):340-344
There has been an increasing importance of drug-resistant pathogens in clinical microbiological and antibacterial research. Indoles and hydrazone-type compounds constitute important classes of compounds in the search for effective agents against multidrug-resistant microbial infections. In this study a series of 1-methylindole-3-carboxaldehyde hydrazone derivatives were evaluated for their in vitro antimicrobial activities using the two-fold serial dilution technique against Staphylococcus aureus, methicillin-resistant S. aureus, methicillin-resistant S. aureus isolate, Escherichia coli, Bacillus subtilis, and Candida albicans. The minimum inhibitory concentration (MIC) of the test compounds and the reference standards sultamicillin, ampicillin, fluconazole, and ciprofloxacin was determined. All compounds possessed a broad spectrum of activity having MIC values of 6.25-100 microg/ml against the tested microorganisms. Aromaticity and disubstitution of the phenyl ring with especially fluorine and chlorine atoms were found to be significant for the antimicrobial activity 相似文献
99.
Our purpose in this study was to investigate the protective effects of selenium and vitamin E on the blood–brain barrier (BBB)
permeability in rats with convulsion under hyperthermic conditions. To eliminate the effect of sex on BBB, we performed our
study on 4- to 5-week-old prepubertal rat pups. Evans-blue was used as a BBB tracer. Convulsions were induced by administration
of i.p. pentylenetetrazol. In the selenium group, 4 ppm selenium was added to the drinking water for 4–5 weeks. Vitamin E
was administered at 700 mg/kg ip. It was shown that the convulsions, both under normothermic and hyperthermic conditions,
caused widespread increase in the BBB permeability (p < 0.05). In addition, a significant difference was observed among female and male rats (f [1, 102] = 6.387, p < 0.05). In convulsions under normothermic conditions, there was a further increase in the BBB permeability (F[3, 102] = 43.534, p < 0.001) and a greater increase of permeability in males compared to females (F[1, 102] = 6.387, p < 0.05). Selenium and vitamin E significantly decreased the BBB destruction caused by convulsions under hyperthermic conditions
in males (p < 0.05). Treatment with selenium or vitamin E has beneficial effects on the BBB breakdown during convulsions. But gender
differences are very important in BBB permeability under pathological conditions and antioxidant treatments. 相似文献
100.
Celik S Gorur S Aslantas O Erdogan S Ocak S Hakverdi S 《Molecular and cellular biochemistry》2007,297(1-2):131-138
Although oxidative damage is known to be involved in inflammatory-mediated tissue destruction, modulation of oxygen free radical
production represents a new approach to the treatment of inflammatory diseases. Caffeic acid phenethyl ester (CAPE), an active
component of propolis from honeybee hives, has antioxidant, anti-inflammatory and antibacterial properties. For that reason,
we aimed to investigate the efficiency of CAPE administration in preventing oxidative damage in pyelonephritis (PYN) caused
by Escherichia coli. In this study, 35 Wistar rats were grouped as follows: control, PYN 24 h, PYN 48 h, PYN 72 h, CAPE 24 h, CAPE 48 h and CAPE
72 h. E. coli (1 × 109 c.f.u.) were inoculated into the rats in both PYN and CAPE groups via urethral catheterization. Ten μM/kg-body weight CAPE
was injected to the rats in all CAPE groups 24 h before E. coli infection, and injections were repeated at 24-h intervals. Rats were sacrificed 24 h, 48 h and 72 h after infection in both
PYN and CAPE groups. Malondialdehyde (MDA) and nitric oxide (NO) levels were significantly increased in kidneys of PYN groups.
The activities of the antioxidant enzymes, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and
xanthine oxidase (XO) were also elevated by E. coli. However, CAPE administration reduced MDA and NO levels, as well as XO activity, although it increased SOD and GSH-Px activities.
Histopathological examination showed that CAPE reduced the inflammation grade induced by E. coli. In conclusion, CAPE administrations decrease the oxidative damage occurring in PYN and therefore could be used for medical
management of bacterial nephropathy. 相似文献