排序方式: 共有218条查询结果,搜索用时 15 毫秒
141.
Madeleine Kok Bas L. J. H. Kietselaer Casper Mihl Sibel Altintas Estelle C. Nijssen Joachim E. Wildberger Marco Das 《PloS one》2015,10(6)
Purpose
It is unclear if prolonged contrast media injection, to improve right ventricular visualization during coronary CT angiography, leads to increased detection of right ventricle pathology. The purpose of this study was to evaluate right ventricle enhancement and subsequent detection of right ventricle disease during coronary CT angiography.Materials and Methods
472 consecutive patients referred for screening coronary CT angiography were retrospectively evaluated. Every patient underwent multidetector-row CT of the coronary arteries: 128x 0.6mm coll., 100-120kV, rot. time 0.28s, ref. mAs 350 and received an individualized (P3T) contrast bolus injection of iodinated contrast medium (300 mgI/ml). Patient data were analyzed to assess right ventricle enhancement (HU) and right ventricle pathology. Image quality was defined good when right ventricle enhancement >200HU, moderate when 140-200HU and poor when <140HU.Results
Good image quality was found in 372 patients, moderate in 80 patients and poor in 20 patients. Mean enhancement of the right ventricle cavity was 268HU±102. Patients received an average bolus of 108±24 ml at an average peak flow rate of 6.1±2.2 ml/s. In only three out of 472 patients (0.63%) pathology of the right ventricle was found (dilatation) No other right ventricle pathology was detected.Conclusion
Right ventricle pathology was detected in three out of 472 patients; the dilatation observed in these three cases may have been picked up even without dedicated enhancement of the right ventricle. Based on our findings, right ventricle enhancement can be omitted during screening coronary CT angiography. 相似文献142.
Sarcosine was recently identified as a differential metabolite that is present in urine in elevated concentration as prostate cancer develops metastases. The aim of this study is to prepare reflectometric interference spectroscopy (RIfS) nanosensors for the detection of sarcosine. Sarcosine imprinted nanoparticles were prepared by two phase miniemulsion polymerization and characterized with transmission electron microscopy, dynamic light scattering, and atomic force microscopy measurements. The glass substrates (10 × 10 mm2) were treated with Piranha solution and incubated in polyethyleneimine solution. The sarcosine imprinted nanoparticles were attached to glass substrates by spin coating of nanoparticle solution. The recognition properties of the nanosensors were evaluated by reflectometric interferometric spectroscopy. To show selectivity of sarcosine imprinted RIfS nanosensor, real‐time l ‐alanine detection was also performed. Sarcosine detection studies were performed from aqueous solution and urine sample. A good linearity was revealed with a correlation coefficient of 0.9622 and a detection limit of 45 nM. © 2014 American Institute of Chemical Engineers Biotechnol. Prog., 31:55–61, 2015 相似文献
143.
Akın Sibel Özer Firüzan Fırat Zararsız Gözde Ertürk Şafak Elif Deniz Mucuk Salime Arguvanlı Sibel Mazıcıoğlu Mümtaz 《Sleep and biological rhythms》2020,18(3):243-248
Sleep and Biological Rhythms - The aim of this study was to examine the association between sleep duration and frailty in community-dwelling Turkish older adults and to determine whether this... 相似文献
144.
Taylor DD Akyol S Gercel-Taylor C 《Journal of immunology (Baltimore, Md. : 1950)》2006,176(3):1534-1542
Exosome release by viable cells is a feature of activated cell types, including tumors, fetal cells, and cells of the immune system. Exosomes critically regulate immune activation, by mediating activation-induced cell death. Fetal cells may mimic these events to selectively delete reactive lymphocytes. In this study the presence and composition of placenta-derived exosomes are demonstrated in the maternal circulation along with their consequences on T cell activation markers. For all pregnant patients, exosomes were isolated from sera obtained between 28 and 30 wk gestation. For pregnant women, subsequently delivering at term, circulating levels of placental exosomes were 1.8 times greater than those delivering preterm (p < 0.0001). Exosomes isolated from pregnancies subsequently delivering at term expressed significantly higher levels of biologically active components, including Fas ligand (FasL) and HLA-DR, than those from pregnancies delivering preterm. Standardizing for protein concentrations, exosomes from term-delivering pregnancies exhibited greater suppression of CD3-zeta and JAK3 than those delivering preterm. The suppression of CD3-zeta and JAK3 correlated with exosome expression levels of FasL (r2= 0.92 and r2= 0.938, respectively). Fractionation of exosomes from term-delivering pregnancies by continuously eluting electrophoresis indicated that intact 42 kD FasL and an unidentified 24-kDa protein were associated with CD3-zeta suppression. Our results demonstrated that exosomes from pregnancies ultimately delivering at term are present at significantly greater concentrations than those from pregnancies delivering preterm; however, exosomes from term-delivering pregnancies also exhibit significantly greater suppression of CD3-zeta and JAK3. 相似文献
145.
Ada AO Kunak SC Hancer F Soydas E Alpar S Gulhan M Iscan M 《Molecular biology reports》2012,39(5):5985-5993
Several studies focused on investigating genetic polymorphisms in order to estimate genetic contribution to lung cancer often
showed conflicting results. In this study, we investigated the role of GSTM1, GSTT1, GSTP1 exon 5 and exon 6 polymorphisms on developing lung cancer and histological subtypes in 213 lung cancer patients and 231 controls.
GSTM1 null, GSTT1 null, and GSTP1 exon 5 variant genotypes did not show a significant risk for developing lung cancer overall. Significant association was
noted between GSTP1 exon 6 variant genotypes and overall lung cancer risk (OR 2.17, 95% CI 1.25–3.78; P = 0.006). These results show that GSTP1 exon 6 polymorphism might be an important factor in determining lung cancer susceptibility in a Turkish population. 相似文献
146.
Guldiken B Sipahi T Guldiken S Ustundag S Budak M Turgut N Ozkan H 《Molecular biology reports》2009,36(6):1539-1543
The low plasma nitric oxide concentrations and reduced vascular reactivity are considered major proatherogenic mechanisms
in cardiovascular diseases. The present study aimed to assess the allelic frequency and the genotypic distribution of the
Glu298Asp gene polymorphism at exon 7 of endothelial nitric oxide synthase (eNOS) gene in Turkish ischemic stroke patients
compared to appropriate healthy controls, and to correlate the genetic findings with stroke subtypes. The study population
included 146 (75 males, 71 females) patients with ischemic stroke which were categorized according to the Trial of ORG 10172
in Acute Stroke Treatment (TOAST) and 133 (34 males, 99 females) healthy subjects. The eNOS polymorphism was identified with
a PCR followed by RFLP with the restriction enzyme BanII. Genotypes were defined as GG, GT, and TT according to the presence of the G and T alleles. In this case-control study,
we did not find any significant difference in either the genotypic distribution or allelic frequency of Glu298Asp gene polymorphism
between the patients and the controls. In addition, there was also no significant difference for the genotype distribution
and the allelic frequency among the stroke subtypes. The results suggested the lack of the association between the Glu298Asp
gene polymorphism and ischemic stroke or subtypes of ischemic stroke in the Turkish population. 相似文献
147.
Gurer-Orhan H Orhan H Suzen S Püsküllü MO Buyukbingol E 《Journal of enzyme inhibition and medicinal chemistry》2006,21(2):241-247
New, except 1d, melatonin analogue benzimidazole derivatives were synthesized and characterized in the present study. The potential role of melatonin as an antioxidant by scavenging and detoxifying ROS raised the possibility that compounds that are analogous to melatonin can also be used for their antioxidant properties. Therefore the antioxidant effects of the newly synthesized compounds were investigated in vitro by means of their inhibitory effect on hydrogen peroxide-induced erythrocyte membrane lipid peroxidation (EMLP) and on various erythrocyte antioxidant enzymes viz. superoxide dismutase (SOD), catalase (CAT) and glucose-6-phosphate dehydrogenase (G6PD). The synthesized benzimidazole derivatives showed remarkable antioxidant activity in vitro in the H2O2-induced EMLP system. Furthermore their effects on various antioxidant enzymes are discussed and evaluated from the perspective of structure- activity relationships. 相似文献
148.
Merson RR Franks DG Karchner SI Hahn ME 《Comparative biochemistry and physiology. Toxicology & pharmacology : CBP》2006,142(1-2):85-94
The aryl hydrocarbon receptor (AHR) and AHR repressor (AHRR) proteins regulate gene expression in response to some halogenated aromatic hydrocarbons and polycyclic aromatic hydrocarbons. The Atlantic killifish is a valuable model of the AHR signaling pathway, but antibodies are not available to fully characterize AHR and AHRR proteins. Using bacterially expressed AHRs, we developed specific and sensitive polyclonal antisera against the killifish AHR1, AHR2, and AHRR. In immunoblots, these antibodies recognized full-length killifish AHR and AHRR proteins synthesized in rabbit reticulocyte lysate, proteins expressed in mammalian cells transfected with killifish AHR and AHRR constructs, and AHR proteins in cytosol preparations from killifish tissues. Killifish AHR1 and AHR2 proteins were detected in brain, gill, kidney, heart, liver, and spleen. Antisera specifically precipitated their respective target proteins in immunoprecipitation experiments with in vitro-expressed proteins. Killifish ARNT2 co-precipitated with AHR1 and AHR2. These sensitive, specific, and versatile antibodies will be valuable to researchers investigating AHR signaling and other physiological processes involving AHR and AHRR proteins. 相似文献
149.
150.
Wetzel MK Naska S Laliberté CL Rymar VV Fujitani M Biernaskie JA Cole CJ Lerch JP Spring S Wang SH Frankland PW Henkelman RM Josselyn SA Sadikot AF Miller FD Kaplan DR 《Neuron》2008,59(5):708-721
The genetic mechanisms that regulate neurodegeneration are only poorly understood. We show that the loss of one allele of the p53 family member, p73, makes mice susceptible to neurodegeneration as a consequence of aging or Alzheimer's disease (AD). Behavioral analyses demonstrated that old, but not young, p73+/- mice displayed reduced motor and cognitive function, CNS atrophy, and neuronal degeneration. Unexpectedly, brains of aged p73+/- mice demonstrated dramatic accumulations of phospho-tau (P-tau)-positive filaments. Moreover, when crossed to a mouse model of AD expressing a mutant amyloid precursor protein, brains of these mice showed neuronal degeneration and early and robust formation of tangle-like structures containing P-tau. The increase in P-tau was likely mediated by JNK; in p73+/- neurons, the activity of the p73 target JNK was enhanced, and JNK regulated P-tau levels. Thus, p73 is essential for preventing neurodegeneration, and haploinsufficiency for p73 may be a susceptibility factor for AD and other neurodegenerative disorders. 相似文献