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31.
Yongtai Yin Zhenyi Guo Kang Chen Tian Tian Jiajun Tan Xinfeng Chen Jing Chen Bing Yang Shuyan Tang Kangfu Peng Si Liu Yu Liang Kai Zhang Longjiang Yu Maoteng Li 《The Plant journal : for cell and molecular biology》2020,103(6):2151-2167
For decades, genetic engineering approaches to produce unusual fatty acids (UFAs) in crops has reached a bottleneck, including reduced seed oil production and seed vigor. Currently, plant models in the field of research are primarily used to investigate defects in oil production and seedling development, while the role of UFAs in embryonic developmental defects remains unknown. In this study, we developed a transgenic Arabidopsis plant model, in which the embryo exhibits severely wrinkled appearance owing to α‐linolenic acid (ALA) accumulation. RNA‐sequencing analysis in the defective embryo suggested that brassinosteroid synthesis, FA synthesis and photosynthesis were inhibited, while FA degradation, endoplasmic reticulum stress and oxidative stress were activated. Lipidomics analysis showed that ultra‐accumulated ALA is released from phosphatidylcholine as a free FA in cells, inducing severe endoplasmic reticulum and oxidative stress. Furthermore, we identified that overexpression of lysophosphatidic acid acyltransferase 2 rescued the defective phenotype. In the rescue line, the pool capacity of the Kennedy pathway was increased, and the esterification of ALA indirectly to triacylglycerol was enhanced to avoid stress. This study provides a plant model that aids in understanding the molecular mechanism of embryonic developmental defects and generates strategies to produce higher levels of UFAs. 相似文献
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Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration. Genetic engineering
technology has been successfully used in many plant species, but it is limited in Hydrangea. Here we established
an efficient regeneration system by using stem segments as explants for the first time. In our study, the plant
growth regulators (PGRs) were evaluated at the different regeneration processes, including axillary shoots regeneration and root induction. We found that the optimal concentration for axillary buds’ induction was 2.0 mgL−1
6-BA and 0.5 mgL−1
1 IAA, its highest induction rate was 70%. Moreover, the highest axillary shoots proliferation
coefficient was 10.7 on the Murashige and Skoog (MS) medium with 2.0 mgL−1 6-benzyladenine (BA), 0.2 mgL−1
indole-3-butyric acid (IBA), and 1.0 mgL−1 gibberellin A3 (GA3). The highest frequency of root induction was
80.0 ± 0.06% by culturing the elongated shoots in 1/2 MS medium containing 0.1 mgL−1 IBA. In summary,
our study will provide an effective technology for large-scale propagation and important pathway for promoting
the popularization and application of Hydrangea bretschneideri Dipp. 相似文献
34.
Dong Chengya Wen Shaohong Zhao Shunying Sun Si Zhao Shangfeng Dong Wen Han Pingxin Chen Qingfang Gong Ting Chen Wentao Liu Wenqian Liu Xiangrong 《Neurochemical research》2021,46(4):755-769
Neurochemical Research - Cerebral ischemia leads to reactive astrogliosis and glial scar formation. Glial scarring can impede functional restoration during the recovery phase of stroke. Salidroside... 相似文献
35.
为构建含东北地区人乳头瘤病毒16型(HPV16)全基因组的HPV16.HaCaT细胞模型,收集中国东北地区HPV16单一感染患者宫颈脱落细胞,提取DNA,将HPV16全基因组分成4个区段,通过4对特异性引物对HPV16全基因组进行分段扩增,测序后进行序列拼接及核酸序列分析,克隆HPV16全基因组序列;通过细胞转染,构建含HPV16全基因组的HPV16.HaCaT重组细胞模型;利用聚合酶链式反应(PCR)和细胞免疫荧光法检测重组细胞内HPV16早期基因的表达.成功克隆出中国东北地区HPV16全基因组序列(GenBank登录号:MW320358);构建了东北地区HPV16全基因组的重组质粒及HPV16.HaCaT重组细胞模型;证明了 HPV16早期基因E1-E4、E5、E6和E7在重组细胞模型内均有表达,从而获得中国东北地区HPV16全基因组序列及含有HPV16全基因组的HPV16.HaCaT重组细胞模型. 相似文献
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Lin Zhu Xiaoyan Yang Juyi Li Xiong Jia Xiangli Bai Ying Zhao Wenzhuo Cheng Meng Shu Yan Zhu Si Jin 《遗传学报》2021,48(2):134-146
Gene therapy has become the most effective treatment for monogenic diseases. Congenital LEPTIN deficiency is a rare autosomal recessive monogenic obesity syndrome caused by mutations in the Leptin gene. Ob/ob mouse is a monogenic obesity model, which carries a homozygous point mutation of C to T in Exon 2 of the Leptin gene. Here, we attempted to edit the mutated Leptin gene in ob/ob mice preadipocytes and inguinal adipose tissues using CRISPR/Cas9 to correct the C to T mutation and restore the production of LEPTIN protein by adipocytes. The edited preadipocytes exhibit a correction of 5.5% of Leptin alleles and produce normal LEPTIN protein when differentiated into mature adipocytes. The ob/ob mice display correction of 1.67% of Leptin alleles, which is sufficient to restore the production and physiological functions of LEPTIN protein, such as suppressing appetite and alleviating insulin resistance. Our study suggests CRISPR/Cas9-mediated in situ genome editing as a feasible therapeutic strategy for human monogenic diseases, and paves the way for further research on efficient delivery system in potential future clinical application. 相似文献
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Liu Na Si Yanhong Zhang Ying Guo Shoudong Qin Shucun 《Journal of physiology and biochemistry》2021,77(4):683-694
Journal of Physiology and Biochemistry - Cholesteryl ester transfer protein (CETP) and phospholipid transfer protein (PLTP) belong to the same gene family. Liver-specific expression of CETP... 相似文献