西藏黄绿卷毛菇生境土壤微生物群落组成

牧草外生菌根菌黄绿卷毛菇Floccularia luteovirens具有较高的生态和经济价值,其生境微生物对其菌丝生长、菌根化和子实体形成具有促进作用。本研究利用高通量测序技术对西藏黄绿卷毛菇菌窝土壤及其周围无菇土壤微生物群落组成进行分析,挖掘促进黄绿卷毛菇生长的有益微生物资源。结果显示,西藏黄绿卷毛菇生境土壤细菌隶属于17门22纲116目161科227属,其中酸杆菌门、变形菌门、拟杆菌门和疣微菌门累计比例高达81.75%;生境土壤真菌隶属于5门17纲34目55科61属,子囊菌门和担子菌门累计比例高达80.89%。对比周围无菇土壤的微生物群落,促进黄绿卷毛菇生长的潜在细菌类群为Flavisolibacter、黄杆菌属Flavobacterium、芽单胞菌属Gemmatimonas、Haliangium、赛格特杆菌Segetibacter和鞘氨醇单胞菌属Sphingomonas;促进黄绿卷毛菇生长的潜在真菌类群为斜盖伞属Clitopilus、丝膜菌属Cortinarius、被孢霉属Mortierella和毛霉属Mucor。土壤微生物促进黄绿卷毛菇生长繁殖的机制有待进一步研究。     

重组内肽酶AspN的原核表达纯化和活性鉴定

蛋白内肽酶AspN是一种锌金属内切肽酶,能选择性切割天冬氨酸的N端肽键,广泛应用于蛋白的多肽制备及质量肽图谱鉴定。目前内肽酶AspN来源于细菌分泌,产量低,制备困难,成本高,极大地限制了该酶的应用。将脑膜脓毒性黄杆菌分泌的蛋白内肽酶Asp N所对应的基因克隆入表达载体p ET32a,导入E.coli BL21(DE3),首次运用原核表达系统进行可溶性融合表达,亲和层析对重组蛋白进行纯化。用HPLC、SDS-PAGE和荧光底物Anthranilyl-Ala-Phe-Ala-Phe-Asp-Val-Phe(NO2)-Tyr-Asp对重组酶进行酶活鉴定。结果表明重组的内肽酶Asp N具有与标准品基本一致的酶切活性,能够较好地应用于生物和制药领域。     

潮汐作用对黄河三角洲盐沼湿地甲烷排放的影响

盐沼湿地作为陆海交互作用的过渡带是CH₄重要的自然来源。潮汐活动通过影响CH₄的产生、氧化和传输驱动了湿地CH₄间歇性、周期性的排放。利用涡度相关和微气象监测技术,对黄河三角洲一个盐地碱蓬生态系统CH₄通量、环境因子和水文要素（潮汐）进行了长期连续监测分析了该生态系统生长季CH₄排放的季节动态及潮汐作用对CH₄排放的影响。结果表明：生长季该生态系统是CH₄的排放源,排放日均值为0.063 mg m^-2 h^-1,（范围为-0.36-0.57 mg m^-2 h^-1）。潮汐淹水阶段和落潮后湿润阶段表现为CH₄的显著源。此外我们发现,短期潮汐活动引起土壤干湿状况的变化促进了CH₄脉冲式的排放,因此未来气候变化下温度升高和降雨季节分配引起的土壤干湿变化将会对该区域CH₄排放甚至碳循环产生积极影响。     

Molecular Engineering of Monodisperse SnO2 Nanocrystals Anchored on Doped Graphene with High‐Performance Lithium/Sodium‐Storage Properties in Half/Full Cells

The fabrication of ultrasmall and high‐content SnO₂ nanocrystals anchored on doped graphene can endow SnO₂ with superior electrochemical properties. Herein, an effective strategy, involving molecular engineering of a layer‐by‐layer assembly technique, is proposed to homogeneously anchor SnO₂ nanocrystals on nitrogen/sulfur codoped graphene (NSGS), which serves as an advanced anode material in lithium/sodium‐ion batteries (LIBs/SIBs). Benefiting from novel design and specific structure, the optimized NSGS for LIBs displays high initial capacity (2123.9 mAh g^?1 at 0.1 A g^?1), long‐term cycling performance (only 0.8% loss after 500 cycles), and good rate capability (477.4 mAh g^?1 at 5 A g^?1). In addition, the optimized NSGS for SIBs also delivers high initial capacity (791.7 mAh g^?1 at 0.1 A g^?1) and high reversible capacity (180.2 mAh g^?1 after 500 cycles at 0.5 A g^?1). Meanwhile, based on the detailed analysis of phase transition and electrochemical reaction kinetics, the reaction mechanisms of NSGS in LIBs and SIBs as well as the distinction in LIBs/SIBs are clearly articulated. Notably, to further explore the practical application, Li/Na⁺ full cells are also assembled by coupling the optimized NSGS anode with LiCoO₂ and Na₃V₂(PO₄)₃/C cathodes, respectively.     

基于投入产出分析的中国生态足迹模型

基于投入产出分析技术的生态足迹（EF）核算模型是目前国际生态承载力研究的热点模型之一。在已有研究的基础上,引入土地利用差异转化系数（某类土地利用的产量因子与其均衡因子的乘积）改进模型,以保证投入产出表的行、列组分加和操作的有效性。研究以货币型投入产出表为基础核算中国1997年的EF,分析表明：（1）1997年中国为EF净出口国,人均EF贸易逆差0．050hm^2,占生产包含足迹的3．4％;（2）最终使用的人均EF为1．435hm^2（能源足迹占45．1％）,其中,2／3缘于居民消费,另1／3缘于资本形成;（3）第一、二、三产业包含的人均EF依次为0．499、0．721hm^2和0．183hm^2。其中,第一产业以直接占用生态空间为主,而第二、三产业以间接占用为主,如第二产业的隐藏性非能源足迹是其直接值的140余倍;（4）三大产业部门中,第三产业最终产品消费的EF最小,人均0．183hm^2,而其土地利用的经济效率最高,为0．898万元hm^-2。实践表明基于投入产出分析的EF模型不仅承袭了EF基本模型的优点,而且结构性能好,能同时识别社会各生产和消费部门的生态压力及其在不同部门之间的流转情况,并可有效避免足迹组分的重复计算。     

北京市消耗食物生态足迹距离

以北京市消耗食物生态足迹的距离为研究核心。结果表明2008年至2012年,北京的生态足迹距离、人均生态足迹和生态足迹总里程总体呈现逐年增长趋势:生态足迹距离增大到676.75 km,增长19.3%;人均生态足迹距离增加到18.42万km,增长54%;生态足迹总里程增加了1倍强,达到56亿t km。北京市的食物生态足迹距离不断扩大,并涵盖了全国大部分地区,北京城市化的生态成本上升。所消耗各类食物的生态足迹距离从大到小分别为水果类、蔬菜或粮油类、肉蛋类和水产类。蔬菜类和水果类的生态足迹距离存在明显的季节波动,且冬春季节远高于夏秋季节。从转移生态承载力来源地的视角,直线距离越远、占据市场份额越大的地区,对北京市消耗食物生态足迹距离的贡献率越高。     

Salmonella Pathogenicity Island 1(SPI-1) at Work

As an indispensable virulence determinant, Salmonella pathogenicity island 1(SPI-1) has gained much attention in host–pathogen interactions. More and more studies on SPI-1 have demonstrated that it is far more complex than the one we used to expect. It not only affects sophisticated activities during infection, including invasion, replication, and host responses, but also extends to other fields like biofilm formation. In addition, mutants in SPI-1 effectors have been explored as vaccines, the effective ways to prevent salmonellosis. The activity of SPI-1 is influenced by many factors which have been widely reported. Investigations on these factors may provide new insights into prevention and treatment of salmonellosis. Therefore, SPI-1 is a tempting issue worthy of further exploration. In this review, we will probe into SPI-1 systematically.