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991.
992.
Père David’s deer underwent known bottlenecks but has recovered to more than 2000 individuals in China, making it interesting
to assess its genetic variability from a microsatellite perspective. For this purpose, we developed eight novel microsatellite
loci using magnetic-bead enrichment methods. These microsatellite markers revealed a low level of genetic variation in the
David’s deer, showing two alleles each locus, 0.31 and 0.38 for average observed and expected heterozyogosities, respectively.
Combined with previous cross-species primers, this set of markers would play an important role in future genetic investigation
of the David’s deer. 相似文献
993.
Zheng Chen Ya-Yun Xu Jin-Fang Ge Fei-Hu Chen 《Cellular and molecular neurobiology》2018,38(3):627-633
The anorexigenic molecule nesfatin-1 has recently been taken as a potential mood regulator, but the potential mechanisms remain unknown. Results of our previous study have demonstrated that nesfatin-1 could induce anxiety- and depression-like behaviors in rats, accompanied by the hyperactivity of the hypothalamic–pituitary–adrenal axis and the imbalanced mRNA expression of synaptic vesicle proteins. To explore the potential neurobiological mechanism underlying the effect of nesfatin-1 on the synaptic plasticity, the human neuroblastoma SH-SY5Y cells were cultured and treated with different concentrations of nesfatin-1 in the present study. The mRNA and protein expressions of corticotropin-releasing hormone (CRH) were measured via real-time fluorescent quantitative PCR and western blot, respectively. The protein expressions of extracellular signal-regulated kinase 1/2 (ERK1/2), phosphorylated-ERK1/2 (p-ERK1/2), and synapsin I were detected via western blot. The results confirmed that nesfatin-1 (10?9~10?7 mol/L) could up-regulate the expression of CRH. Moreover, nesfatin-1 (10?9~10?7 mol/L) could also increase the protein expressions of p-ERK1/2 and synapsin I, and these effects could be blocked by CP376395, a selective antagonist of CRH type 1 receptor (CRHR1). Furthermore, the increased expression of synapsin I induced by nesfatin-1 could also be reversed by PD98059, a specific inhibitor of the p-ERK. These results indicated that CRHR1 might mediate the effect of nesfatin-1 on the expressions of synapsin I via ERK1/2 signaling pathway. 相似文献
994.
Jiang Jiao Hua Ge Guo Gao Kai Pang Ying Chai Rui Chao Jia Xi Hua Kong Jin Ge Yu Albert Cheung-Hoi 《Neurochemical research》2015,40(9):1929-1944
Neurochemical Research - Cadmium (Cd), a highly ubiquitous toxic heavy metal, can contaminate the environment, including agricultural soil, water and air, via industrial runoff and other sources of... 相似文献
995.
Ji-Xia Li Abdelrahman Said Xiu-Guo Ge Wenxiu Wang Yong Zhang Tianming Jin 《Cytotechnology》2018,70(1):67-82
This study aimed to develop a bovine mammary epithelial (BME) cell line model, which provides a possibility to determine functional properties of the bovine mammary gland. The primary cell culture was derived from bovine mammary gland tissues and processed enzymatically to obtain cell colonies with epithelial-like morphology. The cultures of BME cells were purified and optimally cultured at 37 °C in DMEM/F12 medium supplemented with 10% fetal bovine serum. The BME cells were identified as epithelial cell line by the evaluating the expression of keratin-18 using immunofluorescence staining. A novel gene expression system strongly enhances the expression of telomerase, has been used to immortalize BME cell line termed hTBME cell line. Interestingly, telomerase remained active even after over 60 passages of hTBME cell line, required for immortalization of BME cells. In addition, the hTBME cell line was continuously subcultured with a spontaneous epithelial-like morphology, with a great proliferation activity, and without evidence of apoptotic and necrotic effects. Further characterization showed that hTBME cell line can be continuously propagated in culture with constant chromosomal features and without tumorigenic properties. Finally, established hTBME cell line was evaluated for mammary gland specific functions. Our results demonstrated that the hTBME cell line was able to retain functional-morphological structure, and functional differentiation by expression of beta (β)-casein as in the bovine mammary gland in vivo. Taken together, our findings suggest that the established hTBME cell line can serve as a valuable tool for the study of bovine mammary gland functions. 相似文献
996.
Concentrations of 28 polychlorinated biphenyls (PCBs) were assessed in soils from the Midway Atoll in the central North Pacific Ocean. The analytical procedure involved the application of accelerated solvent extraction (ASE) and gas chromatography coupled with ion trap mass spectrometric detection (GC/ITMS) for identification and quantification. Among the 28 PCB congeners studied, 26 of them, except CB195 and CB209, were detected in the analyzed samples at different frequencies. The total concentrations of 28 indicator PCBs (ΣPCBs) ranged from 2.6 to 148.8 ng g−1 with an average value of 50.7 ng g−1 and median of 39.5 ng g−1. Sources and congeners’ pattern of PCB were investigated in the soil of Midway Atoll. The principal component analysis indicated that the compositions of PCBs in most of the soil samples were similar. The total concentrations of PCBs were used to assess the cancer risk probabilities in humans via ingestion, dermal contact and inhalation of soil particles. Very low cancer risk was found in all soil samples caused by ΣPCBs. 相似文献
997.
Yutaka Niihara Junli Ge Oded Shalev Huiyuan Wu Amy Tu Kouichi R Tanaka 《BMC clinical pharmacology》2002,2(1):8-4
Background
Desferrioxamine (DFO) is an important iron chelating agent. It has also been thought of as an agent with anti-oxidant potential as it chelates ferric iron in various parts of the body. However, there is evidence suggesting that it may paradoxically affect red blood cells (RBC) by inducing intracellular oxidant stress. To further understand the mechanism of DFO's interaction with RBC, we conducted a study to determine the effect of DFO upon RBC's redox status. 相似文献998.
Alpha functions are commonly used to describe different receptor channel kinetics (non-NMDA, GABAA and GABAB). In this paper we show that they may be represented as solutions to simple ordinary differential equations. This alternative
method is compared with the commonly used direct summation of the alpha function conductances in a high-level neuronal circuit
model. A parametric study shows that the differential equation method greatly speeds up the previous summation method. The
forward Euler method used to solve this differential equation is shown to be accurate for this type of simulation. The modelling
of NMDA receptor channel kinetics is also discussed.
Received: 18 December 1992/Accepted in revised form: 28 June 1993 相似文献
999.
1000.