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101.
【目的】通过基因工程手段构建生防菌Act12转录调控因子SPA7074缺失突变株,并挖掘其中活性次级代谢产物资源和探讨其活性机理。【方法】利用同源重组方法敲除Act12基因组中可能的Tet R家族转录调控因子编码基因spa7074(accession number:KU955325),平板实验检测缺失突变株发酵液抑菌活性的变化,并通过HPLC比较代谢图谱,然后通过质谱及核磁共振对差异峰对应化合物进行结构鉴定。【结果】SPA7074缺失突变株对几种病原真菌的拮抗活性显著增强,比较代谢图谱表明出现数个差异峰,将最显著差异峰所对应化合物进行分离纯化鉴定,结果为寡霉素D。【结论】本研究通过基因工程手段敲除生防菌株Act12中的负转录调控转录因子,使得突变菌株抑菌活性显著增强,并获得了产量达野生型菌株7倍的寡霉素D高产菌株Δspa7074。 相似文献
102.
The extensive use of phenol compounds and the inability to remove these compounds during wastewater treatment have resulted in the widespread occurrence of phenols in the natural environment. Phenols have been linked to serious risks to human and environmental health. Hence, the need to develop technologies that can effectively remove phenols from wastewater and source waters is a pressing challenge. In this study, light ceramic particles were immersed in activated sludge acclimated to degrade phenol, and microorganisms were allowed to attach to the particles surface to form biofilm. Then the ceramic particles with biofilm were moved into the photolytic circulating-bed biofilm reactor made of quartz glass, which was used for the degradation of phenol by three protocols: photolysis with UV light alone (P), biodegradation alone (B), and the two mechanisms operating simultaneously (photobiodegradation, P&B). The experimental results indicated that phenol removal rate was quickest by B experiment. However, P&B experiment gave more complete mineralization of phenol than that by other protocols. During P&B experiment, the microorganisms grown on porous ceramic carrier still kept the bioactivity degrading phenol, even under UV light irradiation. However, the dominant members of the bacterial community changed dramatically after the intimately coupled photobiodegradation, according to molecular biological analysis to the biofilm. Whereas Beijerinckia sp. was the dominant strain in the inoculum, it was replaced by Thauera sp. MZ1T that played a main role on degrading phenol during P&B experiment. 相似文献
103.
自发性树鼩乳腺肿瘤的特性(英文) 总被引:2,自引:0,他引:2
乳腺癌是严重危害女性健康的常见恶性肿瘤,建立合适的乳腺癌动物模型对于研究人类乳腺癌的生物学机制及发展新的防治方法至关重要。相对于常用的啮齿类动物,树鼩(Tupaia belangeri chinensis,tree shrew)因在进化层次上更接近于人类而可用于建立更适合的乳腺癌模型。该文详细了介绍一例树鼩自发性乳头状良性乳腺肿瘤。免疫组化结果显示该例肿瘤孕激素受体阳性且Ki-67阳性细胞比例显著增加;而活化的Caspase3阳性细胞比例较低;且肿瘤的形态和病理与人导管内乳头状肿瘤非常接近。提示利用树鼩建立乳腺肿瘤模型的可行性。 相似文献
104.
Yan J Wang Q Zheng X Sun H Zhou Y Li D Lin Y Wang X 《Biochemical and biophysical research communications》2012,417(2):842-846
Sensitization of cancer cells to apoptosis induced by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) by luteolin has been suggested by in vitro studies. However, no in vivo experiment has been reported to validate the potentiation effect of luteolin on TRAIL's anticancer activity. In this report, we first confirmed that luteolin potentiates TRAIL-induced cytotoxicity in A549 cells and HeLa cells in association with increased activation of apoptosis. Then we performed an in vivo experiment with a non-small cell lung cancer xenograft mouse model, which showed for the first time that the in vivo anticancer activity of TRAIL was greatly enhanced by luteolin. Compared with that in untreated control or treatment with TRAIL or luteolin alone, inhibition of tumor growth and apoptotic cell death in xenograft tumors were significantly increased in animals receiving combination treatment with TRAIL and luteolin. Data from this study thus provide strong in vivo evidence supporting that luteolin is a potential sensitizer for TRAIL in anticancer therapy. 相似文献
105.
通过逆转录-聚合酶链反应从我国河南省2例重叠感染HCV或HBV/HDV的献血啼中,分离到HBVNS5区的部分cDNA,对其进行序列分析比较,结果表明,河南株HGVNS5工核苷酸与两中国HGV主同源性高于国外代表株(88.5-90.6%),但由核苷酸推导的氨基酸的同源性都无明显的地区性区别。HGVNS5区氨基酸序列较保守,缺乏明显高变区,中国4株HGV在7384位发生了由C→T的变异,从而导致一个人 相似文献
106.
107.
根癌农杆菌介导Bt基因转化水稻的研究 总被引:2,自引:0,他引:2
为了培育出无筛选标记基因的转基因水稻,试验将loxp-hpt-loxp基因与成基因连锁在-起转化水稻方法,得到loxp-hpt—loxp—Bt转基因水稻植株,再与同质的带有ere基因的水稻杂交,以定向删除潮霉素抗性筛选标记。试验表明以水稻品种“皖粳97”为供试材料,将成熟胚来源的愈伤组织用根癌农杆菌EHA105/pCAMBIA1305.1感染后,筛选出抗性愈伤组织并获得再生植株。经PCR验证,得到20棵转基因水稻植株。 相似文献
108.
中国仓鼠卵巢细胞(CHO-K1)经N-甲基-N'-硝基-N-亚硝基胍(MNNG)诱变和6-巯基鸟嘌呤(6-TG)选择,得到稳定的次黄嘌呤磷酸核糖转移酶(HPRT)缺陷细胞株,酶活性仅为野生型的6.5%。用磷酸钙共沉淀法和电脉冲法向HPRT-细胞转移人宫颈癌细胞(HeLaS_3)基因组DNA,纠正了CHO细胞的HPRT缺陷。酶活性提高了6.9倍,达到野生型的45%。用Alu序列探针进行分子杂交,证实经过基因转移并连续传代15次以上的受体细胞中含人DNA序列。表明人的有关基因已稳定地整合到CHO细胞的染色体中。 相似文献
109.
Detection of the synergetic effects between variants, such as single-nucleotide polymorphisms (SNPs), is crucial for understanding the genetic characters of complex diseases. Here, we proposed a two-step approach to detect differentially inherited SNP modules (synergetic SNP units) from a SNP network. First, SNP-SNP interactions are identified based on prior biological knowledge, such as their adjacency on the chromosome or degree of relatedness between the functional relationships of their genes. These interactions form SNP networks. Second, disease-risk SNP modules (or sub-networks) are prioritised by their differentially inherited properties in IBD (Identity by Descent) profiles of affected and unaffected sibpairs. The search process is driven by the disease information and follows the structure of a SNP network. Simulation studies have indicated that this approach achieves high accuracy and a low false-positive rate in the identification of known disease-susceptible SNPs. Applying this method to an alcoholism dataset, we found that flexible patterns of susceptible SNP combinations do play a role in complex diseases, and some known genes were detected through these risk SNP modules. One example is GRM7, a known alcoholism gene successfully detected by a SNP module comprised of two SNPs, but neither of the two SNPs was significantly associated with the disease in single-locus analysis. These identified genes are also enriched in some pathways associated with alcoholism, including the calcium signalling pathway, axon guidance and neuroactive ligand-receptor interaction. The integration of network biology and genetic analysis provides putative functional bridges between genetic variants and candidate genes or pathways, thereby providing new insight into the aetiology of complex diseases. 相似文献
110.
本文对棕黑锦蛇(Elaphe schrenckii)进行分类学等研究,棕黑锦蛇被认为包含两个亚种:指名亚种(Elaphe s.schrenckii)和赤峰亚种(Elaphe s.anomala),但也有学者将后者提升为种,即赤峰锦蛇(Elaphe anomala).有关该种分类问题,仍需要进一步综合考虑形态学和分子系统学分类研究.作为我国东北和华北地区体形最大的蛇种,棕黑锦蛇具有重要的生态和经济价值,但目前对其生物学特性和生态学研究极为有限,人工驯养繁育技术不成熟,过度利用导致野外种群遭到严重破坏.建议加强棕黑锦蛇生态学和人工繁育技术的研究,为有效保护和可持续利用提供科学依据. 相似文献