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161.
Rat Brain Synaptosomal ATP:AMP-Phosphotransferase Activity 总被引:2,自引:1,他引:2
Agnes K. Nagy Terifence A. Shuster Antonio V. Delgado-Escueta 《Journal of neurochemistry》1989,53(4):1166-1172
Adenylate kinase activity (ATP:AMP-phosphotransferase; EC 2.7.4.3) was studied in various subcellular fractions of rat brain tissues. Because of the presence of other adenosine nucleotide-utilizing enzymes, adenylate kinase activity was assayed in both the forward and reverse directions by using coupled enzyme systems and by using a specific adenylate kinase inhibitor, P1,P5-di(adenosine-5') pentaphosphate. As expected, the highest specific adenylate kinase activity (2.89 mumol/min/mg of protein) was detected in the cytosolic brain fraction. However, substantial enzyme activity (0.68 mumol/min/mg) was also found in the intact synaptosomal fraction isolated on Percoll/sucrose gradients. The increased specific enzyme activity of purified synaptosomes and the differences found between the kinetic parameters of the membrane-bound and cytosolic enzyme forms suggest that the synaptosomal adenylate kinase activity cannot be attributed to the small amount of contaminating cytosol present in our preparations. The adenylate kinase enzyme adhered to purified synaptic plasma membranes and was not released by washings with isoosmotic sucrose medium. The facts that the adenylate kinase enzyme activity could be measured in intact synaptosomal preparations and that both its substrates and its inhibitors do not cross intact plasma membranes support the possibility that the synaptosomal adenylate kinase is an ecto-enzyme. 相似文献
162.
降解烤烟秸秆和烟碱菌株的筛选及其产酶特性 总被引:1,自引:0,他引:1
摘要:【目的】为获得能够降解烤烟秸秆和烟碱的菌株,并探索其降解烤烟秸秆的利用途径。【方法】以烤烟秸秆为唯一碳氮源,从烟田土壤中进行了菌株的筛选。采用形态学观察、生理生化特性鉴定、16S rRNA基因序列鉴定等方法对该菌株进行了鉴定,并对其以烤烟秸秆为底物进行液态发酵的产酶活性和木质纤维素降解效果进行了测定。【结果】结果表明:该菌株为巨大芽孢杆菌(Bacillus megaterium)。在以烤烟秸秆为主要营养物质液态发酵条件下该菌株具有较强的木质素降解能力,最大漆酶活力达到418.52 U/L,而木质素过氧化物酶和锰过氧化物酶的最大酶活分别为19.71 U/L 和64.71 U/L。此外,发酵20 d后该菌能够完全降解发酵液中的烟碱。【结论】本研究筛选到了1株能够较好降解烤烟秸秆和完全降解烟碱的巨大芽孢杆菌(Bacillus megaterium),且该菌株具有利用烤烟秸秆生产漆酶的应用价值。 相似文献
163.
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166.
Sam Shuster 《BMJ (Clinical research ed.)》1983,287(6400):1220-1221
167.
Lori Jo Wilmeth Sanjay Shrestha Gilbert Monta?o Jennifer Rashe Charles Bradley Shuster 《Molecular biology of the cell》2010,21(3):380-392
The spatial and temporal coordination of chromosome segregation with cytokinesis is essential to ensure that each daughter cell receives the correct complement of chromosomal and cytoplasmic material. In yeast, mitotic exit and cytokinesis are coordinated by signaling cascades whose terminal components include a nuclear Dbf2-related family kinase and a noncatalytic subunit, Mps one binding (Mob) 1. There are five human Mob1 isoforms, all of which display redundant localization patterns at the spindle poles and kinetochores in early mitosis, and the spindle midzone during cytokinesis. Mob1 shares similar localization patterns to Polo-like kinase (Plk1) and the chromosomal passenger complex (CPC), and although depletion of Plk1 resulted in a loss of Mob1 from the spindle poles, Mob1 recruitment to kinetochores was unaffected. Conversely, disruption of CPC signaling resulted in a loss of Mob1 from kinetochores without disrupting recruitment to the spindle poles. In Mob1-depleted cells, the relocalization of the CPC and mitotic kinesin-like protein (MKLP) 2 to the spindle midzone was delayed during early anaphase, and as a consequence, the midzone recruitment of MKLP1 also was affected. Together, these results suggest that Mob1 and the other mammalian orthologues of the mitotic exit network regulate mitotic progression by facilitating the timely mobilization of the CPC to the spindle midzone. 相似文献
168.
N. V. Kononova A. I. Bobruskin T. I. Kostromina T. D. Melikhova A. A. Vainson E. V. Sveshnikova A. A. Zinchenko A. V. Demin V. A. Martyanov A. M. Shuster D. I. Bairamashvili 《Applied Biochemistry and Microbiology》2010,46(7):726-732
Technology of industrial production of an active pharmaceutical substance (APS) of the recombinant human granulocyte colony-stimulating factor (rhG-CSF) involved the use of a highly productive E. coli strain capable of biosynthesis of rhG-CSF in the form of inclusion bodies (IB). A method of strain cultivation has been described, and methods of IB isolation, industrial-scale purification, filgrastim APS production, and quality control have been developed. Clinical trials of the preparation, carried out in the leading Russian clinics, were successful. Efficiency and safety of the preparation have been confirmed. A ready pharmaceutical form Neupomax® been produced in Russia since 2008 according to the technology developed. 相似文献
169.
Das J Liu C Moquin RV Lin J Furch JA Spergel SH Doweyko AM McIntyre KW Shuster DJ O'Day KD Penhallow B Hung CY Kanner SB Lin TA Dodd JH Barrish JC Wityak J 《Bioorganic & medicinal chemistry letters》2006,16(9):2411-2415
A series of structurally novel aminothiazole based small molecule inhibitors of Itk were prepared to elucidate their structure-activity relationships (SARs), selectivity and cell activity in inhibiting IL-2 secretion in a Jurkat T-cell assay. Compound 2 is identified as a potent and selective Itk inhibitor which inhibits anti-TCR antibody induced IL-2 production in mice in vivo. 相似文献
170.
Lawrence H. Pinto Emily Eaton Bohao Chen Jonah Fleisher Dmitry Shuster Joel McCauley Dalius Kedainis Sandra M. Siepka Kazuhiro Shimomura Eun-Joo Song Aliya Husain Oren J. Lakser Richard W. Mitchell Maria L. Dowell Melanie Brown Blanca Camoretti-Mercado Robert Naclerio Anne I. Sperling Stephen I. Levin Fred W. Turek Julian Solway 《Mammalian genome》2008,19(1):2-14
We mutagenized male BTBR mice with N-ethyl-N-nitrosourea and screened 1315 of their G3 offspring for airway hyperresponsiveness.
A phenovariant G3 mouse with exaggerated methacholine bronchoconstrictor response was identified and his progeny bred in a
nonspecific-pathogen-free (SPF) facility where sentinels tested positive for minute virus of mice and mouse parvovirus and
where softwood bedding was used. The mutant phenotype was inherited through G11 as a single autosomal semidominant mutation
with marked gender restriction, with males exhibiting almost full penetrance and very few females phenotypically abnormal.
Between G11 and G12, facility infection eradication was undertaken and bedding was changed to hardwood. We could no longer
detect airway hyperresponsiveness in more than 37 G12 offspring of 26 hyperresponsive G11 males. Also, we could not identify
the mutant phenotype among offspring of hyperresponsive G8–G10 sires rederived into an SPF facility despite 21 attempts. These
two observations suggest that both genetic and environmental factors were needed for phenotype expression. We suspect that
rederivation into an SPF facility or altered exposure to pathogens or other unidentified substances modified environmental
interactions with the mutant allele, and so resulted in disappearance of the hyperresponsive phenotype. Our experience suggests
that future searches for genes that confer susceptibility for airway hyperresponsiveness might not be able to identify some
genes that confer susceptibility if the searches are performed in SPF facilities. Experimenters are advised to arrange for
multigeneration constancy of mouse care in order to clone mutant genes. Indeed, we were not able to map the mutation before
losing the phenotype. 相似文献