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11.
The phase-resetting experiment was applied to human periodic finger tapping to understand how its rhythm is controlled by
the internal neural clock that is assumed to exist. In the experiment, the right periodic tapping movement was disturbed transiently
by a series of left finger taps in response to impulsive auditory cues presented randomly at various phases within the tapping
cycle. After each left finger tap, the original periodic tapping was reestablished within several tapping cycles. Influences
of the disturbance on the periodic right finger tapping varied depending on the phase of the periodic right finger tapping
at which each left finger tap was made. It was confirmed that the periodic tapping was disturbed not by the auditory cues
but by the left finger taps. Based on this fact, in this paper each single left tap was considered as the stimulus, and the
phase of the periodic tapping of the right index finger when the left tap was executed as the phase of the stimulus. Responses
of the neural activities (magnetoencephalography, MEG), the tapping movement, and the corresponding muscle activities (electromyography)
were simultaneously measured. Phase-resetting curves (PRCs) representing the degree of phase reset as a function of the phase
of the stimulus were obtained both for the left sensorimotor cortex MEG response and for the right index finger tapping response.
The shapes of both PRCs were similar, suggesting that the phase reset of the left sensorimotor cortex activities and that
of the finger tapping rhythm were the same. Four out of eight subjects showed type-0 reset in Winfree's definition, and the
others showed type-1 reset. For general limit-cycle oscillators, type-0 reset is obtained for relatively strong perturbations
and type 1 for weak perturbations. It was shown that the transient response of MEG to the single left tap stimuli in type-0
subjects, where the phase was progressively reset, were different from those in type-1 subjects. Based on detailed analysis
of the differences, a neural network model for the phase reset of the tapping rhythm is proposed.
Received: 10 February 2000 / Accepted in revised form: 15 January 2002 相似文献
12.
Jiro?ManiwaEmail author Shunsuke?Izumi Naoki?Isobe Takato?Terada 《Reproductive biology and endocrinology : RB&E》2005,3(1):23
Background
The objective of this study was to identify substantially increased proteins in bovine cystic follicular fluid (FF) in order to clarify the pathology and etiology of bovine ovarian follicular cysts (BOFC). 相似文献13.
14.
To improve the pentose fermentation rate in Flammulina velutipes, the putative xylose isomerase (XI) gene from Arabidopsis thaliana was cloned and introduced into F. velutipes and the gene expression was evaluated in transformants. mRNA expression of the putative XI gene and XI activity were observed in two transformants, indicating that the putative gene from A. thaliana was successfully expressed in F. velutipes as a xylose isomerase. In addition, ethanol production from xylose was increased in the recombinant strains. This is the first report demonstrating the possibility of using plant genes as candidates for improving the characteristics of F. velutipes. 相似文献
15.
Chromosomal and extrachromosomal synthesis of exfoliative toxin from Staphylococcus hyicus 下载免费PDF全文
Sato H Watanabe T Higuchi K Teruya K Ohtake A Murata Y Saito H Aizawa C Danbara H Maehara N 《Journal of bacteriology》2000,182(14):4096-4100
Evidence for the existence of two molecular species of exfoliative toxin (ET) synthesized by Staphylococcus hyicus (SHET) under chromosomal and plasmid control is presented. Serological evidence that these molecular species of toxins are distinct from each other is given. The molecular weights of SHET from plasmidless strain P-1 (SHETA) and from plasmid-carrying strains P-10 and P-23 (SHETB) were almost equal. Both of the serotypes of SHET exhibited exfoliation in 1-day-old chickens. The plasmid-cured (P(-)) substrains (P-23C1 and P-23C2) of S. hyicus P-23 did not cause exfoliation in 1-day-old chickens, whereas P(-) substrains (P-10C1 and P-10C2) of strain P-10 caused exfoliation, but they decreased their exfoliative activity. These findings suggest that SHETB was synthesized along with SHETA by strain P-10, whereas the P-23 strain synthesized SHETB alone. The plasmid-carrying strain (P-23) as well as the plasmidless strain (P-1) exhibited the typical clinical signs of exudative epidermitis in pigs. However, plasmid-cured (P(-)) substrains of P-23 (P23C1 and P23C2) did not exhibit the typical clinical signs of exudative epidermitis. These findings suggest that SHETA is synthesized under chromosomal control and SHETB is synthesized under plasmid control and that SHET-producing strains can be divided into three groups: SHETA-producing strains, SHETB-producing strains, and strains producing both toxins. 相似文献
16.
17.
Epigenetic regulation of sensory neurogenesis in the dorsal root ganglion cell line ND7 by folic acid 总被引:1,自引:0,他引:1
Boshnjaku V Ichi S Shen YW Puranmalka R Mania-Farnell B McLone DG Tomita T Mayanil CS 《Epigenetics》2011,6(10):1207-1216
The epigenetic mechanism of folic acid (FA) action on dorsal root ganglion (DRG) cell proliferation and sensory neuron differentiation is not well understood. In this study, the ND7 cell line, derived from DRG cells, was used to elucidate this mechanism. In ND7 cells differentiated with dbcAMP and NGF, Hes1 and Pax3 levels decreased, whereas Neurog2 levels showed a modest increase. Chromatin immunoprecipitation (ChIP) assays examining epigenetic marks at the Hes1 promoter showed that FA favored increased H3K9 and H3K19 acetylation and decreased H3K27 methylation. Hence, FA plays a positive role in cell proliferation. In differentiated ND7 cells, H3K27 methylation decreased, whereas H3K9 and H3K18 acetylation increased at the Neurog2 promoter. FA did not favor this phenotypic outcome. Additionally, in differentiated ND7 Neurog2 associated with the NeuroD1 promoter, FA decreased this association. The results suggest that the switch from proliferation to sensory neuron differentiation in DRG cells is regulated by alterations in epigenetic marks, H3K9/18 acetylation and H3K27 methylation, at Hes1 and Neurog2 promoters, as well as by Neurog2 association with NeuroD1 promoter. FA although positive for proliferation, does not appear to play a role in differentiation. 相似文献
18.
Recent studies of the sea urchin embryo have elucidated the mechanisms that localize and pattern its nervous system. These studies have revealed the presence of two overlapping regions of neurogenic potential at the beginning of embryogenesis, each of which becomes progressively restricted by separate, yet linked, signals, including Wnt and subsequently Nodal and BMP. These signals act to specify and localize the embryonic neural fields - the anterior neuroectoderm and the more posterior ciliary band neuroectoderm - during development. Here, we review these conserved nervous system patterning signals and consider how the relationships between them might have changed during deuterostome evolution. 相似文献
19.
Alyssa Enny Kathleen Flaherty Shunsuke Mori Natalie Turner Tetsuya Nakamura 《Development, growth & differentiation》2020,62(5):311-325
The fish fin is a breathtaking repository full of evolutionary diversity, novelty, and convergence. Over 500 million years, the adaptation to novel habitats has provided landscapes of fin diversity. Although comparative anatomy of evolutionarily divergent patterns over centuries has highlighted the fundamental architectures and evolutionary trends of fins, including convergent evolution, the developmental constraints on fin evolution, which bias the evolutionary trajectories of fin morphology, largely remain elusive. Here, we review the evolutionary history, developmental mechanisms, and evolutionary underpinnings of paired fins, illuminating possible developmental constraints on fin evolution. Our compilation of anatomical and genetic knowledge of fin development sheds light on the canalized and the unpredictable aspects of fin shape in evolution. Leveraged by an arsenal of genomic and genetic tools within the working arena of spectacular fin diversity, evolutionary developmental biology embarks on the establishment of conceptual framework for developmental constraints, previously enigmatic properties of evolution. 相似文献
20.
Shunsuke Matsuoka Yoriko Sugiyama Yoshito Shimono Masayuki Ushio Hideyuki Doi 《Environmental microbiology》2021,23(8):4797-4806
Investigation of seasonal variation in fungal communities is essential for understanding biodiversity and ecosystem functions. However, the conventional sampling method, with substrate removal and high spatial heterogeneity of community composition, makes surveying the seasonality of fungal communities challenging. Recently, water environmental DNA (eDNA) analysis has been explored for its utility in biodiversity surveys. In this study, we assessed whether the seasonality of fungal communities can be detected by monitoring eDNA in a forest stream. We conducted monthly water sampling in a forest stream over 2 years and used DNA metabarcoding to identify fungal eDNA. The stream water contained DNA from functionally diverse aquatic and terrestrial fungi, such as plant decomposers, parasites and mutualists. The variation in the fungal assemblage showed a regular annual periodicity, meaning that the assemblages in a given season were similar, irrespective of the year or sampling. Furthermore, the strength of the annual periodicity varied among functional groups. Our results suggest that forest streams may act as a ‘trap’ for terrestrial fungal DNA derived from different habitats, allowing the analysis of fungal DNA in stream water to provide information about the temporal variation in fungal communities in both the aquatic and the surrounding terrestrial ecosystems. 相似文献